Shiokawa et al., 2021 - Google Patents
Bovine endometrium-derived cultured cells are suitable for lipofectionShiokawa et al., 2021
View HTML- Document ID
- 3439335784964440739
- Author
- Shiokawa M
- Miura R
- Okubo A
- Hagita Y
- Yoshimura I
- Aoki H
- Publication year
- Publication venue
- Scientific Reports
External Links
Snippet
Bovine-derived cultured cells, including Madin-Darby bovine kidney cells, are used worldwide; however, lipofection tend to result in low transfection efficiency, which has impeded the progress of veterinary research. We performed experiments to confirm the …
- 238000001638 lipofection 0 title abstract description 86
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
- C12N5/0602—Vertebrate cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic, hydroximic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material Not used, see subgroups
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Williamson et al. | Clinical benefit of remdesivir in rhesus macaques infected with SARS-CoV-2 | |
Zheng et al. | Irradiation induces cancer lung metastasis through activation of the cGAS–STING–CCL5 pathway in mesenchymal stromal cells | |
Moore et al. | Culture of human leukemia cells | |
Cao et al. | Muscle stem cells differentiate into haematopoietic lineages but retain myogenic potential | |
Morshead et al. | Hematopoietic competence is a rare property of neural stem cells that may depend on genetic and epigenetic alterations | |
Clarke et al. | Syringe loading introduces macromolecules into living mammalian cell cytosol | |
Willers et al. | Dissociation of p53-mediated suppression of homologous recombination from G1/S cell cycle checkpoint control | |
Tang et al. | Bacterial magnetic particles as a novel and efficient gene vaccine delivery system | |
Wu et al. | The effect of the microgravity rotating culture system on the chondrogenic differentiation of bone marrow mesenchymal stem cells | |
Xiang et al. | Mesenchymal stem cells as a gene therapy carrier for treatment of fibrosarcoma | |
Cai et al. | The role of Staphylococcus aureus small colony variants in intraosseous invasion and colonization in periprosthetic joint infection | |
Shiokawa et al. | Bovine endometrium-derived cultured cells are suitable for lipofection | |
Miyamoto et al. | M1-like macrophage contributes to chondrogenesis in vitro | |
Tsai et al. | The effect of infection order of porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus on dually infected swine alveolar macrophages | |
Wei et al. | Single-cell profiling of mouse and primate ovaries identifies high levels of EGFR for stromal cells in ovarian aging | |
Mulligan et al. | Porcine reproductive and respiratory virus 2 infection of the fetus results in multi-organ cell cycle suppression | |
Yang et al. | An Estrogen–NK Cells Regulatory Axis in Endometriosis, Related Infertility, and Miscarriage | |
Tan et al. | Regulation of RIP1‐Mediated necroptosis via necrostatin‐1 in periodontitis | |
Walter et al. | A Novel In Vitro Mouse Model to Study Mycobacterium tuberculosis Dissemination Across Brain Vessels: A Combination Granuloma and Blood‐Brain Barrier Mouse Model | |
Peng et al. | Effects of lentiviral transfection containing bFGF gene on the biological characteristics of rabbit BMSCs | |
De Vocht et al. | Multimodal imaging of stem cell implantation in the central nervous system of mice | |
Penolazzi et al. | Influence of obstetric factors on osteogenic potential of umbilical cord-derived mesenchymal stem cells | |
Jia et al. | An optimized method for obtaining clinical‐grade specific cell subpopulations from human umbilical cord‐derived mesenchymal stem cells | |
Shen et al. | Immune Regulation of Seminal Plasma on the Endometrial Microenvironment: Physiological and Pathological Conditions | |
Chen et al. | Live cell imaging of the TGF-β/Smad3 signaling pathway in vitro and in vivo using an adenovirus reporter system |