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Oscorbin et al., 2024 - Google Patents

Optimization of conditions for production of soluble E. coli polyA-polymerase

Oscorbin et al., 2024

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Document ID
2788747060351054707
Author
Oscorbin I
Kunova M
Filipenko M
Publication year
Publication venue
bioRxiv

External Links

Snippet

Poly (A)-polymerase (PAP-1) from Escherichia coli is the primary enzyme responsible for synthesizing poly (A) tails on RNA molecules, signaling RNA degradation in bacterial cells. In vitro, PAP-1 is used to prepare libraries for RNAseq and to produce mRNA vaccines …
Continue reading at www.biorxiv.org (PDF) (other versions)

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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