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Barcelo et al., 2001 - Google Patents

Transformation and gene expression

Barcelo et al., 2001

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Document ID
1992178370695811800
Author
Barcelo P
Rasco-Gaunt S
Thorpe C
Lazzeri P
Publication year

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DuPont Wheat Transformation Laboratory, c/o Rothamsted Experimental Station, Harpenden, Hertfordshire AL5 2JQ, UK Page 1 Transformation and Gene Expression PILAR BARCELO, SONRIZA RASCO-GAUNT, CATHERINE THORPE and PAUL A …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
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