BeMiller et al., 1993 - Google Patents
Suicide-substrate inactivation of β-galactosidase by diazomethyl β-d-galactopyranosyl ketoneBeMiller et al., 1993
- Document ID
- 17757917808725612311
- Author
- BeMiller J
- Gilson R
- Myers R
- Santoro M
- Publication year
- Publication venue
- Carbohydrate research
External Links
Snippet
Diazomethyl β-d-galactopyranosyl ketone (1) has been proven to be a mechanism-based, irreversible (suicide-substrate) inactivator of Aspergillus oryzae β-d-galactosidase, but not an inactivator of E. coli lacZ β-d-galactosidase. Compound 1 is stable in buffers of normal …
- 230000002779 inactivation 0 title abstract description 27
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
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