[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

Liu et al., 2013 - Google Patents

Inheritable and precise large genomic deletions of non-coding RNA genes in zebrafish using TALENs

Liu et al., 2013

View HTML
Document ID
17268116008368724064
Author
Liu Y
Luo D
Zhao H
Zhu Z
Hu W
Cheng C
Publication year
Publication venue
PloS one

External Links

Snippet

Transcription activator-like effector nucleases (TALENs) have so far been applied to disrupt protein-coding genes which constitute only 2–3% of the genome in animals. The majority (70–90%) of the animal genome is actually transcribed as non-coding RNAs (ncRNAs), yet …
Continue reading at journals.plos.org (HTML) (other versions)

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/10—Production naturally occurring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Similar Documents

Publication Publication Date Title
Liu et al. Inheritable and precise large genomic deletions of non-coding RNA genes in zebrafish using TALENs
Renaud et al. Improved genome editing efficiency and flexibility using modified oligonucleotides with TALEN and CRISPR-Cas9 nucleases
Fellmann et al. Cornerstones of CRISPR–Cas in drug discovery and therapy
Chen et al. Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans
Yang et al. One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering
Liu et al. CRISPR/Cas9 in zebrafish: an efficient combination for human genetic diseases modeling
Bassett et al. Understanding functional miRNA–target interactions in vivo by site-specific genome engineering
Nakanishi et al. CRISPR/Cas-mediated targeted mutagenesis in Daphnia magna
Wang et al. Highly efficient CRISPR/HDR-mediated knock-in for mouse embryonic stem cells and zygotes
Wang et al. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering
Thyme et al. Polq-mediated end joining is essential for surviving DNA double-strand breaks during early zebrafish development
Han et al. Efficient in vivo deletion of a large imprinted lncRNA by CRISPR/Cas9
Marques et al. Intergenic lncRNAs and the evolution of gene expression
JP2022033881A (en) Multiplexed genome editing
Frøkjær-Jensen Exciting prospects for precise engineering of Caenorhabditis elegans genomes with CRISPR/Cas9
Park et al. A resource for the conditional ablation of microRNAs in the mouse
Ran et al. Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
Singh et al. A mouse geneticist’s practical guide to CRISPR applications
Bassett et al. Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system
Cerbini et al. Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines
Miano et al. A CRISPR path to engineering new genetic mouse models for cardiovascular research
Ota et al. Multiple genome modifications by the CRISPR/Cas9 system in zebrafish
Wei et al. Heritable genome editing with CRISPR/Cas9 in the silkworm, Bombyx mori
Aalto et al. Opposing roles of microRNA Argonautes during Caenorhabditis elegans aging
Ma et al. High efficiency in vivo genome engineering with a simplified 15-RVD GoldyTALEN design