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Becker et al., 2007 - Google Patents

Ultrahigh‐throughput screening to identify e. coli cells expressing functionally active enzymes on their surface

Becker et al., 2007

Document ID
16755586932923298915
Author
Becker S
Michalczyk A
Wilhelm S
Jaeger K
Kolmar H
Publication year
Publication venue
Chembiochem

External Links

Snippet

We show here that E. coli bacteria that display esterases or lipases on their cell surface together with horseradish peroxidase (HRP) are capable of hydrolysing carboxylic acid esters of biotin tyramide. The tyramide radicals generated by the coupled lipase–peroxidase …
Continue reading at chemistry-europe.onlinelibrary.wiley.com (other versions)

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
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    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

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