Ribeiro-Rodrigues et al., 2020 - Google Patents
Simple and Fast SEC-Based Protocol to Isolate Human Plasma-Derived Extracellular Vesicles for Transcriptional ResearchRibeiro-Rodrigues et al., 2020
View PDF- Document ID
- 15815182557971521573
- Author
- Ribeiro-Rodrigues T
- Girão H
- Nobre R
- de Almeida L
- Publication year
- Publication venue
- Molecular Therapy
External Links
Snippet
Extracellular vesicles (EVs) are membranous structures that protect RNAs from damage when circulating in complex biological fluids, such as plasma. RNAs are extremely specific to health and disease, being powerful tools for diagnosis, treatment response monitoring …
- 230000002103 transcriptional effect 0 title abstract description 17
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Dong et al. | Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium | |
Gaspar et al. | Simple and fast SEC-based protocol to isolate human plasma-derived extracellular vesicles for transcriptional research | |
Welsh et al. | Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches | |
Martins et al. | A review on comparative studies addressing exosome isolation methods from body fluids | |
JP5823031B2 (en) | Vesicle capture device and method for using the same | |
Lee | A simple outline of methods for protein isolation and purification | |
EP3458854B1 (en) | Method and kit for capturing extracellular vesicles (evs) on a solid surface | |
Satori et al. | Review on recent advances in the analysis of isolated organelles | |
CN109642229A (en) | Extracellular vesica is separated from biofluid and isolates the automatically and manually method of Cell-free DNA | |
JP7364240B2 (en) | Method for separating extracellular endoplasmic reticulum using hydrophobic interactions | |
WO2020199633A1 (en) | Methods for lipid affinity-based non-antibody capture and purification of extracellular vesicles | |
Yang et al. | An isolation system to collect high quality and purity extracellular vesicles from serum | |
Chernyshev et al. | Asymmetric depth‐filtration: A versatile and scalable method for high‐yield isolation of extracellular vesicles with low contamination | |
KR102109921B1 (en) | Method for the analysis of extracellular vesicles using size exclusion chromatography and use thereof | |
JP2011010650A (en) | Method for preparing protein, dna and rna from cell | |
JP2023508411A (en) | Methods, automated systems and cartridges for the extraction of cell-free nucleic acids from blood samples | |
Ribeiro-Rodrigues et al. | Simple and Fast SEC-Based Protocol to Isolate Human Plasma-Derived Extracellular Vesicles for Transcriptional Research | |
Gaspar et al. | Simple and Fast Size Exclusion Chromatography-based Protocol to Isolate Human Plasma-derived Extracellular Vesicles for Transcriptional Research | |
JP6437250B2 (en) | Pretreatment method and nucleic acid extraction kit used therefor | |
JP2006343220A (en) | Pretreatment method of biocomponent-containing solution and analysis solution purification method | |
JP7279542B2 (en) | Method for quantifying target cells contained in a sample | |
Chernyshev et al. | Asymmetric depth-filtration–a versatile and scalable approach for isolation and purification of extracellular vesicles | |
Lucas | Catch and Display Profiling of Small Extracellular Vesicles: A Microfluidic Approach for Surveillance of Cancer and Immunity | |
JP2019504987A (en) | Non-recombinant human insulin-like growth factor binding protein concentrate | |
Wehland et al. | Combining Immuno-Affinity Chromatography and Filtration to Improve Specificity and Size Distribution of Exosome-Containing EV Populations |