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Rosson et al., 1995 - Google Patents

Constitutive c-myb expression in K562 cells inhibits induced erythroid differentiation but not tetradecanoyl phorbol acetate-induced megakaryocytic differentiation

Rosson et al., 1995

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Document ID
15463537601567852191
Author
Rosson D
O'Brien T
Publication year
Publication venue
Molecular and cellular biology

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K562 cells were stably transfected with a plasmid vector constitutively expressing a full- length human c-myb gene. Parental cells possess the dual potential of inducibility of cellular differentiation along two lineages, ie, erythroid and megakaryocytic. The resulting lineage is …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602—Vertebrate cells
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