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Shingles et al., 1997 - Google Patents

Measurement of carbonic anhydrase activity using a sensitive fluorometric assay

Shingles et al., 1997

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Document ID
15280182800727738130
Author
Shingles R
Moroney J
Publication year
Publication venue
Analytical biochemistry

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Snippet

The dehydration reaction of bicarbonate was measured using the fluorescent pH indicator, 8- hydroxypyrene-1, 3, 6-trisulfonate (pyranine), in combination with stopped-flow spectrofluorometry. The initial rate of bicarbonate dehydration was measured after mixing a …
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
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