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FAWCETT et al., 1985 - Google Patents

Electrofocusing in wide pH range Immobiline gels containing carrier ampholytes

FAWCETT et al., 1985

Document ID
14729205498484282195
Author
FAWCETT J
CHRAMBACH A
Publication year
Publication venue
Protides of the Biological Fluids

External Links

Snippet

Polyacrylamide gels covalently bonded with Immobiline (LKB) of a wide pH range (4-10) exhibit problems due to low conductance regions within the pH gradient such as slow migration rates across the pH extremes leading to substantial lateral zone diffusion and …
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Classifications

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    • G01N27/26Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
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    • G01N27/44704Details; Accessories
    • G01N27/44747Composition of gel or of carrier mixture
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    • G01N27/00Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
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    • G01N27/416Systems
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    • G01N27/44721Arrangements for investigating the separated zones, e.g. localising zones by optical means
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    • G01N27/44739Collecting the separated zones, e.g. blotting to a membrane or punching of gel spots
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    • G01N33/48Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay
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    • G01N27/00Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/02Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating the impedance of the material
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    • GPHYSICS
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    • G01N27/62Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating the ionisation of gases; by investigating electric discharges, e.g. emission of cathode

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