[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

Huang et al., 1992 - Google Patents

High efficiency cross‐linked polyacrylamide coating for capillary electrophoresis of proteins

Huang et al., 1992

Document ID
1407095665582525970
Author
Huang M
Vorkink W
Lee M
Publication year
Publication venue
Journal of Microcolumn Separations

External Links

Snippet

A highly efficient cross‐linked polyacrylamide capillary coating has been developed for capillary electrophoresis of proteins. The fused silica capillary tubing was pretreated with 7‐ oct‐1‐enyltrimethoxysilane and then statically coated with the polymerization reagents. The …
Continue reading at onlinelibrary.wiley.com (other versions)

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44747Composition of gel or of carrier mixture
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44752Controlling the zeta potential, e.g. by wall coatings
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44717Arrangements for investigating the separated zones, e.g. localising zones
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44743Introducing samples
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/287Non-polar phases; Reversed phases
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography

Similar Documents

Publication Publication Date Title
Huang et al. High efficiency cross‐linked polyacrylamide coating for capillary electrophoresis of proteins
Zhao et al. Adsorption on polymer-coated fused-silica capillary electrophoresis columns using selected protein and peptide standards
Horvath et al. Polymer wall coatings for capillary electrophoresis
Albarghouthi et al. Poly‐N‐hydroxyethylacrylamide as a novel, adsorbed coating for protein separation by capillary electrophoresis
CA2274536C (en) Preformed polymer coating process and product
US5447617A (en) Coated capillary columns and electrophoretic separation methods for their use
US4931328A (en) Capillary tube with reduced protein interactions and controllable electroosmotic flow
JP2000513449A (en) Improved method for capillary electrophoresis in nucleic acids, proteins and small charged compounds
Koide et al. Enantiomeric separations of cationic and neutral compounds by capillary electrochromatography with charged polyacrylamide gels incorporating chiral selectors
Malik et al. Simple method for the preparation of highly efficient polymer‐coated capillary electrophoresis columns
US5006313A (en) Halogenated surface with reduced protein interaction
Ren et al. Poly (ethylene-propylene glycol)-modified fused-silica columns for capillary electrophoresis using epoxy resin as intermediate coating
US5089106A (en) High performance capillary gel electrophoresis
US5314593A (en) Capillary tube with reversible protein interaction and method
EP0452055A1 (en) Surfaces with reduced protein interactions
US5282941A (en) Capillary gel electrophoresis columns and method of preparing the same
Burt et al. Resin coating for capillaries giving a net positive charge and great potential for customised modification of surface properties
Capelli et al. ‘Tunable’positive and negative surface charges on a capillary wall: exploiting the Immobiline chemistry
Finkler et al. Permanent coated capillaries with reversed electroosmotic flow for anion analysis
JPH0718841B2 (en) Method for preparing gel-filled capillary column
Střelec et al. Modification of capillary electrophoresis capillaries by poly (hydroxyethyl methacrylate), poly (diethylene glycol monomethacrylate) and poly (triethylene glycol monomethacrylate)
Fridström et al. Dextran‐modified polypropylene hollow fibers for use in capillary electrophoresis
US5167783A (en) Capillary gel electrophoresis columns and method of preparing the same
Schomburg Problems and achievements in the instrumentation and column technology for chromatography and capillary electrophoresis
Sawada et al. Capillary electrophoretic separation of structurally similar solutes in noncross‐linked poly (acrylamide‐co‐N‐isopropylacrylamide) solution