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Rafalski, 1986 - Google Patents

Structure of wheat gamma-gliadin genes

Rafalski, 1986

Document ID
1307156720534135643
Author
Rafalski J
Publication year
Publication venue
Gene

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Snippet

We have cloned and sequenced two linked members of the wheat (Triticum aestivum) gliadin multigene family. One gene encodes a γ-gliadin which is 292 amino acids (aa) long. S1 mapping indicates that this gene is transcriptionally active. The second gene, which is …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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