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Tsuchiya et al., 2005 - Google Patents

Long-term extensive expansion of mouse hepatic stem/progenitor cells in a novel serum-free culture system

Tsuchiya et al., 2005

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Document ID
12015145217893618224
Author
Tsuchiya A
Heike T
Fujino H
Shiota M
Umeda K
Yoshimoto M
Matsuda Y
Ichida T
Aoyagi Y
Nakahata T
Publication year
Publication venue
Gastroenterology

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Background & Aims: The liver has high regenerative potential. We attempted to establish a novel culture system for extensive expansion of fetal mouse hepatic stem/progenitor cells and to characterize cultured cells. Methods: Hepatic spheroids collected from 6-day floating …
Continue reading at www.gastrojournal.org (HTML) (other versions)

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
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    • C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
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    • C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
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    • C12N2501/39—Steroid hormones
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells

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