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Lindsay et al., 2004 - Google Patents

Combinatorial formulation of biocatalyst preparations for increased activity in organic solvents: salt activation of penicillin amidase

Lindsay et al., 2004

Document ID
11470172878472694148
Author
Lindsay J
Clark D
Dordick J
Publication year
Publication venue
Biotechnology and bioengineering

External Links

Snippet

A combinatorial experimental technique was used to identify salts and salt mixtures capable of activating penicillin amidase in organic solvents for the transesterification of phenoxyacetate methyl ester with 1‐propanol. Penicillin amidase was lyophilized in the …
Continue reading at analyticalsciencejournals.onlinelibrary.wiley.com (other versions)

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells being immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells being immobilised on or in an organic carrier entrapped within the carrier, e.g. gel, hollow fibre
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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells being immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells being immobilised on or in an organic carrier carrier being a synthetic polymer
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions

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