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Li et al., 2013 - Google Patents

Efficient and stable rescue of classical swine fever virus from cloned cDNA using an RNA polymerase II system

Li et al., 2013

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Document ID
10178716657552193071
Author
Li C
Huang J
Li Y
He F
Li D
Sun Y
Han W
Li S
Qiu H
Publication year
Publication venue
Archives of virology

External Links

Snippet

Conventional reverse genetics for classical swine fever virus (CSFV) is based on the transfection of permissive cells with either in vitro or intracellularly synthesized RNA transcripts from a viral genomic cDNA clone. These strategies are complicated, inefficient …
Continue reading at www.researchgate.net (PDF) (other versions)

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N2770/00011—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA Viruses positive-sense ssRNA Viruses positive-sense
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    • C12N2770/24311—Pestivirus, e.g. bovine viral diarrhea virus
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