Tschirpke et al., 2024 - Google Patents
Sortase A-mediated farnesylation of Cdc42 in vitroTschirpke et al., 2024
View PDF- Document ID
- 9737301714873864149
- Author
- Tschirpke S
- Hettema N
- Spitzbarth B
- Eelkema R
- Laan L
- Publication year
- Publication venue
- bioRxiv
External Links
Snippet
Cdc42, a Rho-family GTPase, plays a pivotal role in establishing polarity in Saccharomyces cerevisiae by accumulating on the membrane at the site of bud emergence. Cdc42s ability to bind to membranes, mediated by prenylation, is essential for its function. Prenylation …
- 102000011068 Cdc42 0 title abstract description 272
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US12054541B2 (en) | Split inteins, conjugates and uses thereof | |
JP6883529B2 (en) | Methods and products for synthesizing fusion proteins | |
Heal et al. | N-Myristoyl transferase-mediated protein labelling in vivo | |
US9797889B2 (en) | Activation of bioluminescence by structural complementation | |
Li et al. | Ligation of expressed protein α-hydrazides via genetic incorporation of an α-hydroxy acid | |
US9968690B2 (en) | Norbornene modified peptides and their labelling with tetrazine compounds | |
JP2018502848A (en) | Microbial transglutaminase, its substrate, and method of use | |
US20220135628A1 (en) | Polypeptide with enhanced rate of spontaneous isopeptide bond formation with its peptide tag partner and uses thereof | |
ES2896100T3 (en) | FKBP domain with transglutaminase recognition site | |
Al-Homsi et al. | Construction of pRSET-sfGFP plasmid for fusion-protein expression, purification and detection | |
Suzuki et al. | Methyl-selective isotope labeling using α-ketoisovalerate for the yeast Pichia pastoris recombinant protein expression system | |
US10774039B2 (en) | Cyclopropene amino acids and methods | |
EP3750911A1 (en) | Cysteine-free inteins | |
Tschirpke et al. | Sortase A-mediated farnesylation of Cdc42 in vitro | |
Wiltschi et al. | Expanding the genetic code of Saccharomyces cerevisiae with methionine analogues | |
Khanna et al. | Expression and purification of functional human glycogen synthase-1 (hGYS1) in insect cells | |
KR100708225B1 (en) | Site-specific isotopically-labeled proteins, amino acids, and biochemical precursors therefor | |
Bachmann et al. | Chemical-tag labeling of proteins using fully recombinant split inteins | |
Wang et al. | PAL-Mediated Ligation for Protein and Cell-Surface Modification | |
US20240182530A1 (en) | Polypeptides that interact with peptide tags at loops or termini and uses thereof | |
Fuchs | Enzymatic protein fusions with 100% product yield | |
Moul | Understanding and Exploiting Peroxisomal Import Mechanisms | |
Wu | Techniques for labelling biological macromolecules for spectroscopic studies | |
IL300815A (en) | Modified proteins or peptides for covalent targeting | |
Gui | Development of cell-permeable ubiquitin probes for investigation of deubiquitinases |