[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

Zhang, 2012 - Google Patents

A microfluidic platform to study pathogen-host interactions at single cell level

Zhang, 2012

View PDF
Document ID
8386849310752538163
Author
Zhang R
Publication year

External Links

Snippet

In this study, a high throughput poly (dimethylsiloxane)(PDMS) array chip consisting of multiple micro-wells and microfluidic channels was developed to analyze two biological perspectives of pathogen-host interactions–adherence and gene transcription–at single cell …
Continue reading at dr.ntu.edu.sg (PDF) (other versions)

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated micro-fluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof

Similar Documents

Publication Publication Date Title
Zhang et al. Single-molecule DNA amplification and analysis using microfluidics
US12065640B2 (en) Microfluidic device for extracting, isolating, and analyzing DNA from cells
KR102502083B1 (en) Portable nucleic acid analysis system and high-performance microfluidic electroactive polymer actuators
Pipper et al. Clockwork PCR including sample preparation
AU2007316672B2 (en) Device and process for assays using binding members
KR101702154B1 (en) Device for carrying out a reaction
Chen et al. Specific sorting of single bacterial cells with microfabricated fluorescence-activated cell sorting and tyramide signal amplification fluorescence in situ hybridization
JP2016521350A (en) Method and apparatus for analysis of defined multicellular combinations
CN102264899A (en) Nucleic acid extraction on curved glass surfaces
Basha et al. Towards multiplex molecular diagnosis—A review of microfluidic genomics technologies
Ouyang et al. Pressure-modulated selective electrokinetic trapping for direct enrichment, purification, and detection of nucleic acids in human serum
Su et al. Nucleic acid-based detection for foodborne virus utilizing microfluidic systems
Ven et al. Target confinement in small reaction volumes using microfluidic technologies: a smart approach for single-entity detection and analysis
Kim et al. On-site extraction and purification of bacterial nucleic acids from blood samples using an unpowered microfluidic device
Casavant et al. Efficient sample preparation from complex biological samples using a sliding lid for immobilized droplet extractions
WO2007136303A8 (en) Method for detecting uro-genital infection, oligonucleotide, oligonucleotide combination, biochip and a set based thereon
Zimny et al. Hydrogel droplet single-cell processing: DNA purification, handling, release, and on-chip linearization
Zhang et al. A high-throughput microfluidic biochip to quantify bacterial adhesion to single host cells by real-time PCR assay
Zhang A microfluidic platform to study pathogen-host interactions at single cell level
CN207828268U (en) It is a kind of based on micro-fluidic drop regular array chip
KR20240033032A (en) Highly multiplexed reaction vessels, reagent droppers, and related methods
AU2014200826B2 (en) Assays
Stumpf Automated microfluidic nucleic acid analysis for single-cell and sample-to-answer applications
Berasaluce Vélez de Mendizabal Microfluidic pathogen detection, based on continuous bead beating. DNA concentration and aliquoting for multiplex qPCR detection
JP2010172270A (en) Microreaction vessel, and polymerase chain reaction by using the same