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He et al., 2019 - Google Patents

Boosting activity of high-fidelity CRISPR/Cas9 variants using a tRNAGln-processing system in human cells

He et al., 2019

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Document ID
788790203662479679
Author
He X
Wang Y
Yang F
Wang B
Xie H
Gu L
Zhao T
Liu X
Zhang D
Ren Q
Liu X
Liu Y
Gao C
Gu F
Publication year
Publication venue
Journal of Biological Chemistry

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CRISPR/Cas9 nucleases are widely used for genome editing but can induce unwanted off- target mutations. High-fidelity Cas9 variants have been identified; however, they often have reduced activity, constraining their utility, which presents a major challenge for their use in …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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