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Fu et al., 2006 - Google Patents

Split marker transformation increases homologous integration frequency in Cryptococcus neoformans

Fu et al., 2006

Document ID
7719337584363604613
Author
Fu J
Hettler E
Wickes B
Publication year
Publication venue
Fungal Genetics and Biology

External Links

Snippet

Gene disruption in Cryptococcus neoformans can be problematic due to high frequencies of ectopic integration and telomerization. To improve the frequency of homologous integration, a transformation strategy was employed called split marker, which utilizes a mixture of DNAs …
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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