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Khalilzadeh et al., 2003 - Google Patents

Over-expression of recombinant human interferon-gamma in high cell density fermentation of Escherichia coli

Khalilzadeh et al., 2003

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Document ID
6718682236059407217
Author
Khalilzadeh R
Shojaosadati S
Bahrami A
Maghsoudi N
Publication year
Publication venue
Biotechnology letters

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Snippet

Human interferon-gamma (hIFN-γ) was expressed in Escherichia coli BL21 (DE3) under the control of the T7 promoter. Glucose was used as the sole source of carbon and energy with simple exponential feeding rate in fed-batch process. Cell density of recombinant E. coli was …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12P21/00—Preparation of peptides or proteins
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
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