Van Kaer et al., 1988 - Google Patents
Interaction of the Bacillus subtilis phage phi 105 repressor DNA: a genetic analysis.Van Kaer et al., 1988
View PDF- Document ID
- 6256459026196716368
- Author
- Van Kaer L
- Gansemans Y
- Van Montagu M
- Dhaese P
- Publication year
- Publication venue
- The EMBO Journal
External Links
Snippet
The Bacillus subtilis phage phi 105 repressor specifically recognizes a 14‐bp operator sequence which does not exhibit 2‐fold rotational symmetry. To facilitate a genetic analysis of this sequence‐dependent DNA binding a B. subtilis strain was constructed in which …
- 230000001718 repressive 0 title abstract description 81
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
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