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Van Kaer et al., 1988 - Google Patents

Interaction of the Bacillus subtilis phage phi 105 repressor DNA: a genetic analysis.

Van Kaer et al., 1988

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Document ID
6256459026196716368
Author
Van Kaer L
Gansemans Y
Van Montagu M
Dhaese P
Publication year
Publication venue
The EMBO Journal

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Snippet

The Bacillus subtilis phage phi 105 repressor specifically recognizes a 14‐bp operator sequence which does not exhibit 2‐fold rotational symmetry. To facilitate a genetic analysis of this sequence‐dependent DNA binding a B. subtilis strain was constructed in which …
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
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    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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