[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

Yang et al., 2022 - Google Patents

Csm6-DNAzyme tandem assay for one-pot and sensitive analysis of lead pollution and bioaccumulation in mice

Yang et al., 2022

View PDF
Document ID
5781540977878724877
Author
Yang H
Li F
Xue T
Khan M
Xia X
Busquets R
Gao H
Dong Y
Zhou W
Deng R
Publication year
Publication venue
Analytical Chemistry

External Links

Snippet

Lead contamination in the environment tends to enter the food chain and further into the human body, causing serious health issues. Herein, we proposed a Csm6-DNAzyme tandem assay (termed cDNAzyme) using CRISPR/Cas III-A Csm6 and GR-5 DNAzyme …
Continue reading at eprints.kingston.ac.uk (PDF) (other versions)

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay
    • G01N33/543—Immunoassay; Biospecific binding assay with an insoluble carrier for immobilising immunochemicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the means of detection
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving viable micro-organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Similar Documents

Publication Publication Date Title
Zhang et al. Reverse transcription recombinase polymerase amplification coupled with CRISPR-Cas12a for facile and highly sensitive colorimetric SARS-CoV-2 detection
Yang et al. Stochastic DNA dual-walkers for ultrafast colorimetric bacteria detection
McConnell et al. Biosensing with dnazymes
Tang et al. Colorimetric detection of kanamycin residue in foods based on the aptamer-enhanced peroxidase-mimicking activity of layered WS2 nanosheets
Sheng et al. MXene coupled with CRISPR-Cas12a for analysis of endotoxin and bacteria
Zong et al. Adsorption of arsenite on gold nanoparticles studied with DNA oligonucleotide probes
Yang et al. Csm6-DNAzyme tandem assay for one-pot and sensitive analysis of lead pollution and bioaccumulation in mice
Zeng et al. Highly sensitive aptasensor for trace arsenic (III) detection using DNAzyme as the biocatalytic amplifier
Huang et al. Target-responsive DNAzyme cross-linked hydrogel for visual quantitative detection of lead
Wu et al. Activity-based DNA-gold nanoparticle probe as colorimetric biosensor for DNA methyltransferase/glycosylase assay
Huang et al. CRISPR-Cas12a-assisted multicolor biosensor for semiquantitative point-of-use testing of the nopaline synthase terminator in genetically modified crops by unaided eyes
Lin et al. Colorimetric sensing of silver (I) and mercury (II) ions based on an assembly of tween 20-stabilized gold nanoparticles
Song et al. Visualized detection of Vibrio parahaemolyticus in food samples using dual-functional aptamers and cut-assisted rolling circle amplification
Xu et al. DNAzyme-based cascade signal amplification strategy for highly sensitive detection of lead ions in the environment
He et al. An electrochemical sensing method based on CRISPR/Cas12a system and hairpin DNA probe for rapid and sensitive detection of Salmonella Typhimurium
Hang et al. Exonuclease III-assisted CRISPR/Cas12a electrochemiluminescence biosensor for sub-femtomolar mercury ions determination
Lin et al. Colorimetric determination of DNA methylation based on the strength of the hydrophobic interactions between DNA and gold nanoparticles
Yang et al. Amplification-free, single-microbead-based Cas12a assay for one-step DNA detection at the single-molecule level
Jiang et al. An Ultrasensitive Colorimetric Foodborne pathogenic detection method using a CRISPR/Cas12a mediated strand Displacement/Hybridization chain reaction
Zhang et al. Double isothermal amplification and CRISPR-Cas12a for sensitive detection of citrinin
Huang et al. A label-free electrochemical sensor for detection of mercury (II) ions based on the direct growth of guanine nanowire
Cai et al. Sensitive detection of Salmonella based on CRISPR-Cas12a and the tetrahedral DNA nanostructure-mediated hyperbranched hybridization chain reaction
Liu et al. A label-free electrochemical sensor for the detection of two kinds of targets based on CRISPR/Cas12a system
Yue et al. A triple amplification strategy using GR-5 DNAzyme as a signal medium for ultrasensitive detection of trace Pb2+ based on CRISPR/Cas12a empowered electrochemical biosensor
Chen et al. DNA Extraction-and Amplification-Free Nucleic Acid Biosensor for the Detection of Foodborne Pathogens Based on CRISPR/Cas12a and Argonaute Protein-Mediated Cascade Signal Amplification