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Yu et al., 2022 - Google Patents

Establishment of a pig CRISPR/Cas9 knockout library for functional gene screening in pig cells

Yu et al., 2022

Document ID
5692133236594699298
Author
Yu C
Zhong H
Yang X
Li G
Wu Z
Yang H
Publication year
Publication venue
Biotechnology Journal

External Links

Snippet

Background As an important farm animal, pig functional genomic study can help understand the molecular mechanism related to the key economic traits of pig, such as growth, reproduction, or disease. The genome‐scale library based on clustered regularly …
Continue reading at onlinelibrary.wiley.com (other versions)

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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