[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

Gardini et al., 2018 - Google Patents

Three-dimensional tracking of quantum dot-conjugated molecules in living cells

Gardini et al., 2018

Document ID
5350394157521085509
Author
Gardini L
Calamai M
Hatakeyama H
Kanzaki M
Capitanio M
Pavone F
Publication year
Publication venue
Nanoscale Imaging: Methods and Protocols

External Links

Snippet

Here, we describe protocols for three-dimensional tracking of single quantum dot- conjugated molecules with nanometer accuracy in living cells using conventional fluorescence microscopy. The technique exploits out-of-focus images of single emitters …
Continue reading at link.springer.com (other versions)

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay
    • G01N33/536—Immunoassay; Biospecific binding assay with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultra-violet illumination; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence

Similar Documents

Publication Publication Date Title
Sauer et al. Single-molecule localization microscopy in eukaryotes
Thiele et al. Confocal fluorescence-lifetime single-molecule localization microscopy
Lord et al. Single-molecule spectroscopy and imaging of biomolecules in living cells
Stender et al. Single cell optical imaging and spectroscopy
Müller et al. STED microscopy and its applications: new insights into cellular processes on the nanoscale
Appelhans et al. Nanoscale organization of mitochondrial microcompartments revealed by combining tracking and localization microscopy
Hennig et al. Instant live-cell super-resolution imaging of cellular structures by nanoinjection of fluorescent probes
Fitzpatrick et al. STED nanoscopy in living cells using fluorogen activating proteins
Oleksiievets et al. Single-molecule fluorescence lifetime imaging using wide-field and confocal-laser scanning microscopy: A comparative analysis
Liu et al. Fluorescent bioconjugates for super-resolution optical nanoscopy
Gardini et al. 3D tracking of single nanoparticles and quantum dots in living cells by out-of-focus imaging with diffraction pattern recognition
Annibale et al. Identification of the factors affecting co-localization precision for quantitative multicolor localization microscopy
Requejo-Isidro Fluorescence nanoscopy. Methods and applications
Xu et al. Labeling cytosolic targets in live cells with blinking probes
Martin-Fernandez et al. Single molecule fluorescence detection and tracking in mammalian cells: the state-of-the-art and future perspectives
Grunwald et al. Quantum-yield-optimized fluorophores for site-specific labeling and super-resolution imaging
Wu et al. Fluorescent polymer dot-based multicolor stimulated emission depletion nanoscopy with a single laser beam pair for cellular tracking
Gardini et al. Three-dimensional tracking of quantum dot-conjugated molecules in living cells
Gu et al. Simultaneous single-particle superlocalization and rotational tracking
Xiang et al. Single molecules are your quanta: A bottom-up approach toward multidimensional super-resolution microscopy
Schirripa Spagnolo et al. Choosing the probe for single-molecule fluorescence microscopy
Lee et al. Nanoscopic cellular imaging: confinement broadens understanding
Hauke et al. Metal-induced energy transfer (MIET) for live-cell imaging with fluorescent proteins
Galiani et al. Super-resolution microscopy and studies of peroxisomes
Chang et al. A bright light to reveal mobility: single quantum dot tracking reveals membrane dynamics and cellular mechanisms