Inouye et al., 1977 - Google Patents
Alkaline phosphatase synthesis in a cell-free system using DNA and RNA templatesInouye et al., 1977
- Document ID
- 4879868731236648882
- Author
- Inouye H
- Pratt C
- Beckwith J
- Torriani A
- Publication year
- Publication venue
- Journal of Molecular Biology
External Links
Snippet
We have demonstrated the synthesis of the enzyme, alkaline phosphatase, in a cell-free system using 80dphoA DNA as a template. We have detected both monomers of the enzyme, using a subunit rescue method, and active dimeric enzyme. The identity of the …
- 230000015572 biosynthetic process 0 title abstract description 63
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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