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Zhang et al., 2011 - Google Patents

Cloning and biochemical properties of a highly thermostable and enantioselective nitrilase from Alcaligenes sp. ECU0401 and its potential for (R)-(−)-mandelic acid …

Zhang et al., 2011

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Document ID
4526454986164175139
Author
Zhang Z
Xu J
He Y
Ouyang L
Liu Y
Publication year
Publication venue
Bioprocess and biosystems engineering

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Snippet

A nitrilase gene from Alcaligenes sp. ECU0401 was cloned and overexpressed in Escherichia coli BL21 (DE3) in a soluble form. The encoded protein with a His6-tag was purified to nearly homogeneity as revealed by SDS-PAGE with a molecular weight of …
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