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Reduction of phosphorylated Thr-161 Cdk1 level participates in roscovitine-induced Fas ligand-mediated apoptosis in rat eggs cultured in vitro

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Abstract

The present study was aimed to find out whether roscovitine reduces phosphorylated Thr-161 of cyclin-dependent kinase 1 (Cdk1) level and induces egg apoptosis through Fas ligand (FasL)-mediated pathway. For this purpose, ovulated eggs were cultured in media 199 with or without various concentrations of roscovitine (0, 25, 50, 100, 200 μM) for 3 h in vitro. The morphological apoptotic changes, phosphorylation status of Cdk1, FasL concentration, caspase-8 and caspase-3 activities, and DNA fragmentation were analyzed. Data of the present study suggest that roscovitine significantly reduced Thr-161 phosphorylated Cdk1 level without altering the total level of Cdk1 and induced cytoplasmic fragmentation, a morphological apoptotic feature in a concentration-dependent manner. The roscovitine-induced cytoplasmic fragmentation was associated with increased FasL concentration. The increased FasL concentration induced caspase-8 followed by caspase-3 activities. The increased caspases activity finally induced DNA fragmentation in eggs that showed cytoplasmic fragmentation. Taken together, these results suggest that roscovitine reduced phosphorylated Thr-161 of Cdk1 level and induces apoptosis through FasL-mediated pathway in rat eggs cultured in vitro.

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Authors’ contributions

AT conducted all the experiments and wrote the manuscript under the direction of SKC. Both the authors read and approved the final manuscript.

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The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Cell Physiology Laboratory, Biochemistry Unit, Department of Zoology, Banaras Hindu University, Varanasi, 221005, India

    Anima Tripathi & Shail K. Chaube

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  1. Anima Tripathi
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  2. Shail K. Chaube
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Correspondence to Shail K. Chaube.

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Editor: T. Okamoto

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Tripathi, A., Chaube, S.K. Reduction of phosphorylated Thr-161 Cdk1 level participates in roscovitine-induced Fas ligand-mediated apoptosis in rat eggs cultured in vitro. In Vitro Cell.Dev.Biol.-Animal 51, 174–182 (2015). https://doi.org/10.1007/s11626-014-9812-8

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  • DOI: https://doi.org/10.1007/s11626-014-9812-8

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