Abstract
Elicitation effect of silver nano particles (AgNPs) and triggering of defence system by production of hydrogen peroxide (H2O2) as a signaling molecule in the regulation of the activity of stress-related enzymes and production of Taxol was evaluated in suspension- cultured hazel cells (Corylus avellana L.). The cells were treated with different concentrations of AgNPs (0, 2.5, 5, and 10 ppm), in their logarithmic growth phase (d7) and were harvested after 1 week. Treatment of hazel cells with AgNPs decreased the viability of the cells. Also the results showed that while the activity of certain radical scavenging enzymes in particular of catalase and peroxidase increased by 2.5 and 5 ppm AgNPs, the activity of superoxide dismutase decreased in these treatments. The highest activity of ascorbate peroxidase was observed in 10 ppm AgNPs treatments. This treatment also showed the highest contents of H2O2 and phenolic compounds, as well as the highest activity of phenylalanine ammonialyase. According to the results, 5 ppm AgNPs was the best concentration for elicitation of hazel cells to produce efficient amounts of H2O2 in order for stimulation of antioxidant defence system, production of Taxol at the highest capacity of the cells, meanwhile reserving their viability.
Similar content being viewed by others
Abbreviations
- AgNPs:
-
Silver nano particles
- APX:
-
Ascorbate peroxidase
- CAT:
-
Catalase
- H2O2 :
-
Hydrogen peroxide
- NBT:
-
Nitroblue tetrazolium
- PAL:
-
Phenylalanine ammonia lyase
- POD:
-
Peroxidase
- ROS:
-
Reactive oxygen species
- SOD:
-
Superoxide dismutase
References
Ahmed M, Posgai R, Gorey TJ, Nielsen M, Hussain SM, Rowe JJ (2010) Silver nanoparticles induced heat shock protein 70, oxidative stress and apoptosis in Drosophila melanogaster. Toxicol Appl Pharmacol 242:263–269
An J, Zhang M, Wang S, Tang J (2008) Physical, chemical and microbiological changes in stored green asparagus spears as affected by coating of silver nanoparticles—PVP. LWT Food Sci Technol 41:1100–1107
Armstrong N, Ramamoorthy M, Lyon D, Jones K, Duttaroy A (2013) Mechanism of silver nanoparticles action on insect pigmentation reveals intervention of copper homeostasis. PLoS One 8:e53186. doi:10.1371/journal.pone.0053186
Bemani E, Ghanati F, Rezaei A, Jamshidi M (2013) Effect of phenylalanine on Taxol production and antioxidant activity of extracts of suspension-cultured hazel (Corylus avellana L.) cells. J Nat Med 67:446–451
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Dat J, Vandenabeele S, Vranová E, Van Montagu M, Inzé D, Van Breusegem F (2000) Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 57:779–795
De Pinto M, Tommasi F, De Gara L (2002) Changes in the antioxidant systems as part of the signaling pathway responsible for the programmed cell death activated by nitric oxide and reactive oxygen species in tobacco bright-yellow 2 cells. Plant Physiol 130:698–708
Dewez D, Oukarroum A (2012) Silver nanoparticles toxicity effect on photosystem II photochemistry of the green alga Chlamydomonas reinhardtii treated in light and dark conditions. Toxicol Environ Chem 94:1536–1546
Ghanati F, Bakhtiarian S (2013) Changes of natural compounds of Artemisia annua L. by methyl jasmonate and silver nanoparticles. Adv Environ Biol 7:2251–2258
Ghanati F, Morita A, Yokota H (2005) Effects of aluminum on the growth of tea plant and activation of antioxidant system. Plant Soil 276:133–141
Johnston HJ, Hutchison G, Christensen FM, Peters S, Hankin S, Stone V (2010) A review of the in vivo and in vitro toxicity of silver and gold particulates: particle attributes and biological mechanisms responsible for the observed toxicity. Crit Rev Toxicol 40:328–346
Kasapoglu M, Ozben T (2001) Alterations of antioxidant enzymes and oxidative stress markers in aging. Exp Gerontol 36:209–220
Kim S, Choi JE, Choi J, Chung KH, Park K, Yi J, Ryu DY (2009) Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicol In Vitro 23:1076–1084
Kumar RR, Sharma SK, Gadpayle KA, Singh K, Sivaranjani R, Goswami S, Rai RD (2012) Mechanism of action of hydrogen peroxide in wheat thermotolerance-interaction between antioxidant isoenzymes, proline, and cell membrane. Afr J Biotechnol 11:14368–14379
Lü P, He S, Li H, Cao J, Xu H (2010) Effects of nano-silver treatment on vase life of cut rose cv. Movie Star flowers. J Food Agric Environ 8:1118–1122
Massarsky A, Dupuis L, Taylor J, Eisa-Beygi S, Strek L, Trudeau VL, Moon TW (2013) Assessment of nanosilver toxicity during zebrafish (Danio rerio) development. Chemosphere 92:59–66
Mejía-Teniente L, de Dalia Duran-Flores F, Chapa-Oliver AM, Torres-Pacheco I, Cruz-Hernández A, González-Chavira MM, Ocampo-Velázquez RV, Guevara-González RG (2013) Oxidative and molecular responses in Capsicum annuum L. after hydrogen peroxide, salicylic acid and chitosan foliar applications. Int J Mol Sci 14:10178–10196
Navarro E, Piccapietra F, Wagner B, Marconi F, Kaegi R, Odzak N, Sigg L, Behra R (2008) Toxicity of silver nanoparticles to Chlamydomonas reinhardtii. Environ Sci Technol 42:8959–8964
Nel A, Xia T, Madler L, Li N (2006) Toxic potential of materials at the nanolevel. Science 311:622–627
Pellinen RI, Korhonen MS, Tauriainen AA, Palva ET, Kangasjärvi J (2002) Hydrogen peroxide activates cell death and defense gene expression in Birch. Plant Physiol 130:549–560
Rezaei A, Ghanati F, Behmanesh M, Mokhtari Dizaji M (2011) Ultrasound-potentiated salicylic acid-induced physiological effects and production of Taxol in hazel (Corylus avellana L.) cell culture. Ultrasound Med Biol 37:1938–1947
Sahebjamei H, Abdolmaleki P, Ghanati F (2007) Effects of magnetic field on the antioxidant enzyme activities of suspension-cultured tobacco cells. Bioelectromagnetics 28:42–47
Sondi I, Salopek-Sondi B (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci 285:148–177
Wang JW, Zheng LP, Wu JY, Tan RX (2006) Involvement of nitric oxide in oxidative burst, phenylalanine ammonia-lyase activation and Taxol production induced by low-energy ultrasound in Taxus yunnanensis cell suspension cultures. Nitric Oxide 15:351–358
Wu Y, Zhou Q (2013) Silver nanoparticles cause oxidative damage and histological changes in medaka (Oryzias latipes) after 14 days of exposure. Environ Toxicol Chem 32:165–173
Acknowledgments
This work was supported by grant no. 90000972 from the Iran National Science Foundation (INSF) to FGH on “The effects of MeJA and SNPs on medicinal compounds of plants”.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Jamshidi, M., Ghanati, F., Rezaei, A. et al. Change of antioxidant enzymes activity of hazel (Corylus avellana L.) cells by AgNPs. Cytotechnology 68, 525–530 (2016). https://doi.org/10.1007/s10616-014-9808-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10616-014-9808-y