[go: up one dir, main page]
More Web Proxy on the site http://driver.im/
Skip to main content

Advertisement

Springer Nature Link
Log in

Ultrasensitive cloth-based microfluidic chemiluminescence detection of Listeria monocytogenes hlyA gene by hemin/G-quadruplex DNAzyme and hybridization chain reaction signal amplification

  • Research Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

A Correction to this article was published on 06 April 2022

This article has been updated

Abstract

In this work, a cloth-based chemiluminescence (CL) biosensor has been firstly presented for highly sensitive determination of long PCR amplicons. Under the action of a hybridization chain reaction, a good deal of hemin/G-quadruplex DNAzyme molecules are produced, which can effectively enhance the CL signal. Moreover, effective cloth-based DNA biosensors can be fabricated by sequential wax screen-printing and surface-modification processes. Especially, the integration of a desirable hydrophobic barrier and gravity/capillary flow onto the flow channel of the cloth-based device makes the biosensor easy to be fabricated and to be associated with a flow CL. For the luminol/H2O2-based CL system, the signals are triggered by the hemin/G-quadruplex DNAzyme and are recorded by a low-cost CCD. Under optimized conditions, the determination range of target DNA is 0.002–20,000 pM and its limit of detection is calculated to be 1.1 fM. The results show that the proposed CL biosensor has a good analytical performance, such as high detectability and specificity, wide linear range, and receivable reproducibility and stability. Finally, the proposed biosensor is proven by the fact that this method can successfully detect the target DNA prepared from the Listeria monocytogenes–spiked milk samples. Therefore, it is believed to have the potential application prospects in food safety and environmental monitoring.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
£29.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (United Kingdom)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

Change history

References

  1. Velusamy V, Arshak K, Korostynska O, Oliwa K, Adley C. An overview of foodborne pathogen detection: in the perspective of biosensors. Biotechnol Adv. 2010;28:232–54.

    CAS  PubMed  Google Scholar 

  2. Chemburu S, Wilkins E, Abdel-Hamid I. Detection of pathogenic bacteria in food samples using highly-dispersed carbon particles. Biosens Bioelectron. 2005;21:491–9.

    CAS  PubMed  Google Scholar 

  3. Marušić A. Food safety and security: what were favourite topics for research in the last decade? J Glob Health. 2011;1:72–8.

    PubMed  PubMed Central  Google Scholar 

  4. Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson MA, Roy SL, et al. Foodborne illness acquired in the United States-major pathogens. Emerg Infect Dis. 2011;17:7–15.

    PubMed  PubMed Central  Google Scholar 

  5. Gehring AG, Tu SI. High-throughput biosensors for multiplexed food-borne pathogen detection. Annu Rev Anal Chem. 2011;4:151–72.

    CAS  Google Scholar 

  6. de Boer E, Beumer RR. Methodology for detection and typing of foodborne microorganisms. Int J Food Microbiol. 1999;50:119–30.

    PubMed  Google Scholar 

  7. Sanders SQ, Boothe DH, Frank JF, Arnold JW. Culture and detection of Campylobacter jejuni within mixed microbial populations of biofilms on stainless steel. J Food Prot. 2007;70:1379–85.

    PubMed  Google Scholar 

  8. Chen CS, Durst RA. Simultaneous detection of Escherichia coli O157: H7, Salmonella spp. and Listeria monocytogenes with an array-based immunosorbent assay using universal protein G-liposomal nanovesicles. Talanta. 2006;69:232–8.

    CAS  PubMed  Google Scholar 

  9. Magliulo M, Simoni P, Guardigli M, Michelini E, Luciani M, Lelli R, et al. A rapid multiplexed chemiluminescent immunoassay for the detection of Escherichia coli O157:H7, Yersinia enterocolitica, Salmonella typhimurium, and Listeria monocytogenes pathogen bacteria. J Agric Food Chem. 2007;55:4933–9.

    CAS  PubMed  Google Scholar 

  10. Toze S. PCR and the detection of microbial pathogens in water and wastewater. Water Res. 1999;33:3545–56.

    CAS  Google Scholar 

  11. Kim JS, Lee GG, Park JS, Jung YH, Kwak HS, Kim SB, et al. A novel multiplex PCR assay for rapid and simultaneous detection of five pathogenic bacteria: Escherichia coli O157:H7, Salmonella, Staphylococcus aureus, Listeria monocytogenes, and Vibrio parahaemolyticus. J Food Prot. 2007;70:1656–62.

    CAS  PubMed  Google Scholar 

  12. Zhang P, Zhang CS, Shu BW. Micropatterned paper devices using amine-terminated polydiacetylene vesicles as colorimetric probes for enhanced detection of double-stranded DNA. Sensors Actuators B Chem. 2016;236:27–34.

    CAS  Google Scholar 

  13. Scida K, Li BL, Ellington AD, Crooks RM. DNA detection using origami paper analytical devices. Anal Chem. 2013;85:9713–20.

    CAS  PubMed  Google Scholar 

  14. Zhang W, Luo CH, Zhong L, Nie SC, Cheng W, Zhao D, et al. Sensitive detection of enteropathogenic E. coli using a bfpA gene-based electrochemical sensor. Microchim Acta. 2013;180:1233–40.

    CAS  Google Scholar 

  15. Liu Y, Wei YD, Cao YJ, Zhu DB, Ma WG, Yu Y, et al. Ultrasensitive electrochemiluminescence detection of Staphylococcus aureus via enzyme-free branched DNA signal amplification probe. Biosens Bioelectron. 2018;117:830–7.

    CAS  PubMed  Google Scholar 

  16. Mirasoli M, Bonvicini F, Dolci LS, Zangheri M, Gallinella G, Roda A. Portable chemiluminescence multiplex biosensor for quantitative detection of three B19 DNA genotypes. Anal Bioanal Chem. 2013;405:1139–43.

    CAS  PubMed  Google Scholar 

  17. Liu FF, Zhang CS. A novel paper-based microfluidic enhanced chemiluminescence biosensor for facile, reliable and highly-sensitive gene detection of Listeria monocytogenes. Sensors Actuators B Chem. 2015;209:399–406.

    CAS  Google Scholar 

  18. Kim MS, Kim J. Multiplexed detection of pathogen-specific DNA using engineered zinc finger proteins without target amplification. Anal Methods. 2016;8:6696–700.

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Wang CG, Xiao R, Dong PT, Wu XZ, Rong Z, Xin L, et al. Ultra-sensitive, high-throughput detection of infectious diarrheal diseases by portable chemiluminescence imaging. Biosens Bioelectron. 2014;57:36–40.

    PubMed  Google Scholar 

  20. Donhauser SC, Niessner R, Seidel M. Sensitive quantification of Escherichia coli O157:H7, Salmonella enterica, and Campylobacter jejuni by combining stopped polymerase chain reaction with chemiluminescence flow-through DNA microarray analysis. Anal Chem. 2011;83:3153–60.

    CAS  PubMed  Google Scholar 

  21. Zhu NN, Chang Z, He PG, Fang YZ. Electrochemical DNA biosensors based on platinum nanoparticles combined carbon nanotubes. Anal Chim Acta. 2005;545:21–6.

    CAS  Google Scholar 

  22. Li CH, Xiao X, Tao J, Wang DM, Huang CZ, Zhen SJ. A graphene oxide-based strand displacement amplification platform for ricin detection using aptamer as recognition element. Biosens Bioelectron. 2017;91:149–54.

    PubMed  Google Scholar 

  23. Xu H, Wu D, Jiang YF, Zhang RB, Wu QZ, Liu YY, et al. Loopback rolling circle amplification for ultrasensitive detection of Kras gene. Talanta. 2017;164:511–7.

    CAS  PubMed  Google Scholar 

  24. Fan FX, Du PC, Kan B, Yan MY. The development and evaluation of a loop-mediated isothermal amplification method for the rapid detection of Salmonella enterica serovar typhi. PLoS One. 2015;10:e0124507.

    PubMed  PubMed Central  Google Scholar 

  25. Dirks RM, Pierce NA. Triggered amplification by hybridization chain reaction. Proc Natl Acad Sci U S A. 2004;101:15275–8.

    CAS  PubMed  PubMed Central  Google Scholar 

  26. Wang X, Lau C, Kai M, Lu JZ. Hybridization chain reaction-based instantaneous derivatization technology for chemiluminescence detection of specific DNA sequences. Analyst. 2013;138:2691–7.

    CAS  PubMed  Google Scholar 

  27. Liu X, Shua HL, Liu YJ, Huang KJ. An electrochemical biosensor for DNA detection based on tungsten disulfide/multi-walled carbon nanotube composites and hybridization chain reaction amplification. Sensors Actuators B Chem. 2016;235:603–13.

    CAS  Google Scholar 

  28. Qi HJ, Yue SZ, Bi S, Song WL, Ding CF. A versatile homogeneous chemiluminescence biosensing platform based on exonuclease-assisted hybridization chain reaction. Sensors Actuators B Chem. 2018;273:1525–31.

    CAS  Google Scholar 

  29. Niu SY, Jiang Y, Zhang SS. Fluorescence detection for DNA using hybridization chain reaction with enzyme-amplification. Chem Commun. 2010;46:3089–91.

    CAS  Google Scholar 

  30. Zhao XH, Gong L, Zhang XB, Yang B, Fu T, Hu R, et al. Versatile DNAzyme-based amplified biosensing platforms for nucleic acid, protein, and enzyme activity detection. Anal Chem. 2013;85:3614–20.

    CAS  PubMed  Google Scholar 

  31. Gao Y, Li BX. G-quadruplex DNAzyme-based chemiluminescence biosensing strategy for ultrasensitive DNA detection: combination of exonuclease III-assisted signal amplification and carbon nanotubes-assisted background reducing. Anal Chem. 2013;85:11494–500.

    CAS  PubMed  Google Scholar 

  32. Xiao Y, Pavlov V, Niazov T, Dishon A, Kotler M, Willner I. Catalytic beacons for the detection of DNA and telomerase activity. J Am Chem Soc. 2004;126:7430–1.

    CAS  PubMed  Google Scholar 

  33. Bhandari P, Narahari T, Dendukuri D. 'Fab-Chips': a versatile, fabric-based platform for low-cost, rapid and multiplexed diagnostics. Lab Chip. 2011;11:2493–9.

    CAS  PubMed  Google Scholar 

  34. Malon RSP, Chua KY, Wicakson DHB, Córcoles EP. Cotton fabric-based electrochemical device for lactate measurement in saliva. Analyst. 2014;139:3009–16.

    CAS  PubMed  Google Scholar 

  35. Narahari T, Dendukuri D, Murthy SK. Tunable electrophoretic separations using a scalable, fabric-based platform. Anal Chem. 2015;87:2480–7.

    CAS  PubMed  Google Scholar 

  36. Guan WR, Zhang CS, Liu FF, Liu M. Chemiluminescence detection for microfluidic cloth-based analytical devices (μCADs). Biosens Bioelectron. 2015;72:114–20.

    CAS  PubMed  Google Scholar 

  37. Guan WR, Liu M, Zhang CS. Electrochemiluminescence detection in microfluidic cloth-based analytical devices. Biosens Bioelectron. 2016;75:247–53.

    CAS  PubMed  Google Scholar 

  38. Liu M, Liu R, Wang D, Liu CL, Zhang CS. A low-cost, ultraflexible cloth-based microfluidic device for wireless electrochemiluminescence application. Lab Chip. 2016;16:2860–70.

    CAS  PubMed  Google Scholar 

  39. Liu M, Wang D, Liu CL, Liu R, Li HJ, Zhang CS. Battery-triggered open wireless electrochemiluminescence in a microfluidic cloth-based bipolar device. Sensors Actuators B Chem. 2017;246:327–35.

    CAS  Google Scholar 

  40. Sheng H, Ye BC. Different strategies of covalent attachment of oligonucleotide probe onto glass beads and the hybridization properties. Appl Biochem Biotechnol. 2009;152:54–65.

    CAS  PubMed  Google Scholar 

  41. Preuschoff F, Spohn U, Blankenstein G, Mohr KH, Maria-Regina Kula MR. Chemiluminometric hydrogen peroxide sensor for flow injection analysis. Fresenius J Anal Chem. 1993;346:924–9.

    CAS  Google Scholar 

Download references

Funding

This research is supported by the National Natural Science Foundation of China (81571765) and Guangdong Basic and Applied Basic Research Foundation (2019A1515011284).

Author information

Author notes

  1. Qiuping Shang, Yan Su, and Yi Liang contributed equally to this work.

Authors and Affiliations

  1. MOE Key Laboratory of Laser Life Science & Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, No. 55, Zhongshan Avenue West, Tianhe District, Guangzhou, 510631, Guangdong, China

    Qiuping Shang, Yan Su, Yi Liang, Wei Lai, Jun Jiang, Hongyang Wu & Chunsun Zhang

Authors
  1. Qiuping Shang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yan Su
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yi Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wei Lai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jun Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hongyang Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Chunsun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chunsun Zhang.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

ESM 1

(PDF 445 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shang, Q., Su, Y., Liang, Y. et al. Ultrasensitive cloth-based microfluidic chemiluminescence detection of Listeria monocytogenes hlyA gene by hemin/G-quadruplex DNAzyme and hybridization chain reaction signal amplification. Anal Bioanal Chem 412, 3787–3797 (2020). https://doi.org/10.1007/s00216-020-02633-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-020-02633-5

Keywords

Search

Navigation