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Production, characterisation and applications of monoclonal antibodies to two novel porcine bocaviruses from swine in Northern Ireland

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Abstract

The production, preliminary characterisation and applications of monoclonal antibodies (mAbs) against two novel swine bocaviruses isolated in cell culture from swine in Northern Ireland are described. Of the 17 stable final clones produced, four were characterised. All were of the IgG2a isotype and showed no cross-reactivity with either bocavirus strain. Partial neutralisation was observed with PBoV4 mAbs and homologous virus. The two mAbs selected for use in antigen-detecting ELISAs were successful in highlighting those fractions containing infectious virus within sucrose gradients. This is the first report of the production of specific reagents that will prove useful in the study of the biology of these viruses and swine bocavirus-associated diseases.

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Acknowledgments

This work was funded by the Biotechnology and Biological Sciences Research Council (BBSRC).

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Authors and Affiliations

  1. Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, Belfast, BT4 3SD, Northern Ireland, UK

    I. McNair, F. McNeilly, J. McKillen & M. Welsh

  2. Veterinary Sciences Division, Queens University Belfast, Stormont, Belfast, BT4 3SD, Northern Ireland, UK

    C. Duffy, M. McMenamy & G. Allan

Authors
  1. I. McNair
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  2. F. McNeilly
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  3. C. Duffy
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  4. J. McKillen
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  5. M. McMenamy
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  6. M. Welsh
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  7. G. Allan
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Correspondence to I. McNair.

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McNair, I., McNeilly, F., Duffy, C. et al. Production, characterisation and applications of monoclonal antibodies to two novel porcine bocaviruses from swine in Northern Ireland. Arch Virol 156, 2157–2162 (2011). https://doi.org/10.1007/s00705-011-1107-8

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  • DOI: https://doi.org/10.1007/s00705-011-1107-8

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