Abstract
Interest in malondialdehyde (MDA) metabolism stems from its formation as a product of lipid peroxidation in the diet and in the tissues; its reactivity with functional groups of nucleic acid bases, proteins and phospholipids; its mutagenicity in bacteria, and its reported skin and liver carcinogenicity in animals. Administration of the Na enol salt of MDA in the drinking water of mice over a range of 0.1–10.0 μg/g/day for 12 mo produced dose-dependent hyperplastic and neoplastic changes in liver nuclei and increased mortality at the highest level but produced no gross hepatic tumors. Addition of MDA to the medium of rat skin fibroblasts grown in culture caused nuclear abnormalities at concentrations as low as 10−6M despite an uptake of only 4%. [1,3-14C]MDA was rapidly oxidized to [14C]acetate in rat liver mitochondria and to14CO2 in vivo: however, ∼10% of the radioactivity was recovered in the urine. Chromatographic analysis of rat urine revealed the presence of several compounds which yield MDA on acid hydrolysis. Total MDA excretion increased in response to conditions which stimulate lipid peroxidation in vivo, including vitamin E deficiency, Fe or CCl4 administration, and enrichment of the tissues with PUFA. N-acetyl-e-(2-propenal)lysine was identified as a major urinary metabolite of MDA in rat and human urine. This compound is derived primarily from N-α-(2-propenal)lysine released in digestion as a product of reactions between MDA and the ε-amino groups of N-terminal lysine residues in food proteins. However, its presence in the urine of animals fasted or fed MDA-free diets indicates that it is also formed in vivo. Identification of the metabolites of MDA excreted in the urine may provide clues to the mechanisms of cellular damage caused by this compound in the tissues.
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An erratum to this article is available at http://dx.doi.org/10.1007/BF02535705.
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Draper, H.H., McGirr, L.G. & Hadley, M. The metabolism of malondialdehyde. Lipids 21, 305–307 (1986). https://doi.org/10.1007/BF02536418
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DOI: https://doi.org/10.1007/BF02536418