Abstract
Two extracellular proteolytic activities were characterized fromLeuconostoc oenos isolated from Argentinian wines. Both activities were maximal with autoclaved grape juice as substrate. The temperature and pH optima for the two proteolytic activities were different (30 and 40°C, and pH 4.0 and 5.5, respectively). Both enzymes were thermostable and their activities were unaltered by heating at 70°C for 15 min. Metal ions were not required for the activities. Neither enzyme appeared to be a serine protease but both were strongly inhibited by cysteine and β-mercaptoethanol, indicating the involvement of disulphide bridges.
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References
AyoraS., LindgrenP. & GötzF. 1994 Biochemical properties of a novel metalloprotease fromStaphylococcus hyicus subsp.hyicus involved in extracellular lipase processing.Journal of Bacteriology 176, 3218–3223.
CittiJ.E., SandineW.E. & EllikerP.R. 1963 Some observations on the Hull method for measurement of proteolysis in milk.Journal of Dairy Science 46, 337–342.
CowmanR.A., SwaisgoodH.E. & SpeckM.L. 1967 Proteinase enzyme system of lactic Streptococci. II. Role of membrane proteinase in cellular function.Journal of Bacteriology 94, 942–948.
DrapeauG.R. 1978 The primary structure of staphylococcal protease.Canadian Journal of Biochemistry 56, 534–544.
HullM.E. 1947 Studies on milk proteins. II. Colorimetric determination on the partial hydrolysis of the proteins in milk.Journal of Dairy Science 30, 881–884.
RollánG.C., FaríasM.E. & Manca De NadraM.C. 1993 Protease production byLeuconostoc oenos strains isolated from wine.World Journal of Microbiology and Biotechnology 9, 587–589.
TeufelP. & GötzF. 1993 Characterization of an extracellular metalloprotease with elastase activity fromStaphylococcus epidermis.Journal of Bacteriology 175, 4218–4224.
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The authors are with the Centro de Referencia para Lactobacilos (CERELA), Chacabuco 145, 4000 Tucumán, Argentina M.E. Farías and M.C. Manca De Nadra are also with the Facultad de Bioquímica, Quimica y Farmacia, Universidad Nacional de Tucumán, 4000 Tucumán, Argentina.
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Rollán, G.C., Farías, M.E. & De Nadra, M.C.M. Characterization of two extracellular proteases fromLeuconostoc oenos . World J Microbiol Biotechnol 11, 153–155 (1995). https://doi.org/10.1007/BF00704637
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DOI: https://doi.org/10.1007/BF00704637