Abstract
Rates of denitrification measured over a growing season, both within and between rows of plants, in grass and lucerne leys and in barley plots were related to soil moisture and nitrate levels using bivariate, nonlinear regression models. Both within and between rows in all three crops, moisture explained a significant fraction of the variation in denitrification rates when the rates were regressed as increasing exponentially with increasing soil water content under moist conditions. Only soil moisture explained a significant fraction of the variation of the denitrification rates measured in the soil cores taken in (19.5%) and between (46.3%) plant rows at the barley field and in (42.4%) the plant rows in the lucerne ley. In some treatments, the rates appeared to be unrelated to moisture below a critical moisture threshold.
Nitrate proved to be a useful variable in predicting denitrification rates in the grass ley and between lucerne rows. Water and nitrate could explained 36.6% of the within-row variation and 24.2% of the between-row variation in the grass ley and 65% of the between-row sum of squares.
An attempt to use the regression model based on data from one year for the grass ley to predict the losses during another year for the same crop was not successful.
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References
Aulakh M S, Rennie D A and Paul E A 1982 Gaseous losses from cropped and summer fallowed soils. Can. J. Soil Sci. 62, 187–196.
Bard Y 1974 Nonlinear Parameter Estimation. Academic Press, New York.
Cawse P A and Sheldon D 1972 Rapid reduction of nitrate in soil re-moistened after air-drying. J. Agric. Sci. Camb. 78. 405–412.
Elliot P W, Knight D and Anderson J M 1990. Denitrification in earthworm casts and soil from pastures under different fertilizer and drainage regimes. Soil Biol. Biochem. 22, 601–605.
Firestone M K 1982 Biological denitrification In Nitrogen in Agricultural Soils. Ed. F J Stevenson. Agronomy 22, 289–326.
Folorunso O A and Rolston D E 1984 Spatial variability of field-measured denitrification gas fluxes. Soil Sci. Soc. Am. J. 48, 1214–1219.
Groffman P M, Tiedje J M, Robertsson G P and Christensen C 1987 Denitrification at different temporal and geographical scales: Proximal and distal controls. In Advances in Nitrogen Cycling in Agricultural Ecosystems. Ed. J R Wilsson. pp 174–192. CAB International, Wallingford, UK.
Hansson A-C, Andrén O, Boström S, Boström U, Clarholm M, Lagerlöf J, Lindberg T, Paustian K, Pettersson R and Sohlenius B 1990. Structure of the agroecosystem. In Ecology of Arable Land-Organisms, Carbon and Nitrogen Cycling. Eds. O Andrén, T Lindberg, K Paustian and T Rosswall. Ecol. Bull. (Copenhagen) 40, 41–83.
Johnsson H, Klemedtsson L, Nilsson Å and Svensson B H 1991 Simulation of field scale denitrification losses from soils with grass ley and barley. Plant and Soil 138, 287–302.
Klemedtsson L, Svensson B H, Lindberg T and Rosswall T 1977 The use of acetylene inhibition of nitrous oxide reductase in quantifying denitrification in soils. Swedish J. Agric. Res. 7, 179–185.
Klemedtsson L, Svensson B H and Rosswall T 1988 Relationships between soil moisture content and nitrous oxide production during nitrification and denitrification. Biol. Fertil. Soils 6, 106–111.
McConnaughey P K and Bouldin D R 1985 Transient microsite models of denitrification. I. Model development. Soil Sci. Soc. Am. J. 49, 886–891.
Mosier A R, Parton W J and Hutchinson G L 1983 Modelling nitrous oxide evolution from cropped and native soils. In Environmental Biogeochemistry. Ed. R. Hallberg. Ecol. Bull. (Stockholm) 35, 229–241.
Mosier A R, Stillwell M, Parton W J and K G Woodmansee 1981 Nitrous oxide emission from a native shortgrass prairie. Soil Sci. Soc. Am. J. 45, 617–619.
Myers R J K and McGarity J W 1972 Denitrification in undisturbed cores from a solodized solonetz B horizon. Plant and Soil 37, 81–89.
Myrold D D and Tiedje J M 1985a Establishment of denitrification capacity in soil: Effects of carbon, nitrate and moisture. Soil Ecol. Biochem. 17, 819–822.
Myrold D D and Tiedje J M 1985b Diffusional constraints on denitrification in soils. Soil Sci. Soc. Am. J. 49, 651–657.
Nömmik H 1956 Investigation on denitrification in soil. Acta Agric. Scand. 6, 195–228.
O'Hara G W, Daniel R M and Steale K M 1983 Effect of oxygen on the synthesis activity and breakdown of the Rhizobium denitrification system. J. Gen. Microbiol. 129, 2405–2412.
Orchard V A and Cook F J 1983 Relationships between soil respiration and soil moisture. Soil Biol. Biochem. 15, 447–453.
Parkin T B 1987 Soil microsites as a source of denitrification variability. Soil Sci. Soc. Am. J. 51, 1194–1199.
Rice W C, Sierzega P E, Tiedje J M and Jacobs L W 1988 Stimulated denitrification in the microenvironment of organic wastes injected into soil. Soil Sci. Soc. Am. J. 52, 1492–1501.
Rolstone D E, Rao P A C, Davidsson J M and Jessup R E 1984 Simulation of denitrification losses of nitrate fertilizer applied to uncropped, cropped, and manure-amended field plots. Soil Sci. 137, 270–279.
Rolstone D E, Sharpley A N, Toy D W and Broadbent F E 1982 Field measurement of denitrification. III. Rates during irrigation cycles. Soil Sci. Soc. Am. J. 46, 289–296.
Ruzicka J and Hansen E H 1975 Flow injection analysis. Part I: A new concept of fast continuous flow analysis. Anal. Chim. Acta 78, 145–157.
Ryden J C 1981 N2O exchange between a grassland soil and the atmosphere. Nature 292, 235–237.
Ryden J C, Lund L J, Letey J and Focht D D 1979 Direct measurement of denitrification loss from soils. II. Development and application of field methods. Soil Sci. Soc. Am. J. 43, 110–118.
Sextone A J, Parkin T P and Tiedje J M 1985 Temporal response of soil denitrification rates to rainfall and irrigation. Soil Sci. Soc. Am. J. 49, 99–103.
Sextone A J, Parkin T B and Tiedje J M 1988 Denitrification response to wetting in aggregated and unaggregated soil. Soil Biol. Biochem. 20, 767–769.
Simkins S and Alexander M 1984 Models for mineralization kinetics with the variables of substrate concentration and population density. Appl. Environ. Microbiol. 47, 1299–1306.
Steen E, Jansson P-E and Persson J 1984 Experimental site of the ‘Ecology of Arable Land’ project. Acta Agric. Scand. 34, 156–166.
Stefanson R C 1972 Soil denitrification in sealed soil plant systems. I. Effect of plants: Soil water content and soil organic matter content. Plant and Soil 33, 113–127.
Svensson B H, Boström U and Klemedtsson L 1986 Potential for higher rates of denitrification in earthworm casts than in the surrounding soil. Biol. Fertil. Soils 2, 147–149.
Svensson B H, Klemedtsson L and Rosswall T 1985 Preliminary field denitrification studies of nitrate-fertilized and nitrogen-fixing crops. In Denitrification in the Nitrogen Cycle. Ed. H L Golterman. 99, 157–169. NATO Conference Series I: Ecology Vol. 9. Plenum Press, New York, London.
Svensson B H, Klemedtsson L, Simkins S and Rosswall T 1991 Soil denitrification in three cropping systems characterized by differences in nitrogen and carbon supply. I. Rate-distribution frequencies, comparison between systems and seasonal N-losses. Plant and Soil 138, 257–271.
Woldendorp I W 1963 The Influence of Living Plants on Denitrification. Mededelingen Landbouwhogeschool Wageningen 63, 1–100.
Yoshinari T and R Knowles 1976 Acetylene inhibition of nitrous oxide reduction by denitrifying bacteria. Biochem. Biophys. Res. Comm. 69, 705–710.
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Klemedtsson, L., Simkins, S., Svensson, B.H. et al. Soil denitrification in three cropping systems characterized by differences in nitrogen and carbon supply. Plant Soil 138, 273–286 (1991). https://doi.org/10.1007/BF00012254
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DOI: https://doi.org/10.1007/BF00012254