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Toll-Like Receptors: Ligands, Cell-Based Models, and Readouts for Receptor Action

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Toll-Like Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1390))

Abstract

This chapter details Toll-like receptors (TLRs) and the tools available to study their biology in vitro. Key parameters to consider before exploring TLR action such as receptor localization, signaling pathways, nature of ligands and cellular expression are introduced. Cellular models (i.e., host cells and readouts) based on the use of cell lines, primary cells, or whole blood are presented. The use of modified TLRs to circumvent some technical problems is also discussed.

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Authors and Affiliations

  1. Hudson Institute of Medical Research, Monash University, 27-31 Wright St., Clayton, Melbourne, VIC, 3168, Australia

    Jennifer K. Dowling

  2. Blood Assay Solutions, Toulouse Cedex, France

    Jérome Dellacasagrande

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    Claire E. McCoy

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Dowling, J.K., Dellacasagrande, J. (2016). Toll-Like Receptors: Ligands, Cell-Based Models, and Readouts for Receptor Action. In: McCoy, C. (eds) Toll-Like Receptors. Methods in Molecular Biology, vol 1390. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3335-8_1

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