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Identification and evaluation of potential bio-control fungal endophytes against Ustilagonoidea virens on rice plants

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Abstract

False smut disease of rice is posing an increasing concern for production, not only because of the hiking epidemic occurrence in rice production, but also because of the challenging specific pathogenesis of the disease. The aim of this work was to evaluate the potential of five fungal endophytes to reduce negative effects of rice false smut fungus (Ustilagonoidea virens) on rice plants, in both the laboratory and greenhouse. Though all the fungal isolates showed the ability to inhibit the growth of U. virens with varying degrees, isolate E337 showed significant antagonistic activity against the pathogenic fungi. The isolate E337 was identified as Antennariella placitae by molecular and morphological data analysis including 18S rDNA sequence analysis. This isolate showed a significant in vitro inhibition of mycelial growth of U. virens by dual culture method and it was subsequently tested for its in vivo biocontrol potential on false smut disease on rice plants. Greenhouse experiments confirmed that applications of conidia of A. placitae protected rice plants by improving rice yield and by decreasing the severity of false smut disease on susceptible rice plants. This is the first report where A. placitae has been identified as a biocontrol organism.

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Acknowledgements

This work was supported by the National Natural Sciences Foundation of China (30970627) and the fund for the Important Direction Research on Knowledge Innovation Project (KSCX2-EW-N-06) from CAS awarded to J.X. Li. M. Andargie was a recipient of the ‘Visiting Fellowship for Researchers from Developing Countries’ Award (2013FFSA0005) from Chinese Academy of Sciences.

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Authors and Affiliations

  1. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Mebeaselassie Andargie, Zhu Congyi, Yun Yun & Jianxiong Li

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  1. Mebeaselassie Andargie
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  2. Zhu Congyi
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  3. Yun Yun
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  4. Jianxiong Li
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Correspondence to Jianxiong Li.

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Andargie, M., Congyi, Z., Yun, Y. et al. Identification and evaluation of potential bio-control fungal endophytes against Ustilagonoidea virens on rice plants. World J Microbiol Biotechnol 33, 120 (2017). https://doi.org/10.1007/s11274-017-2273-y

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