Abstract
Crop loss due to pests could reach as high as 70% if preventive measures with either pesticide, natural enemies, host plant resistance or other nonchemical controls are not used. Even if several conventional crop protection methods are in place, Bacillus thuringiensis (Bt)-based bioprotection of crops has been taking prominence. However, several studies documented that pests are yet developing resistance to Bt crops or Bt-based biopesticides. Hence, there is still a need to explore other alternative effective strategies that could stand on its own or could be integrated with other pest management tools. To the effect, gene pyramiding has recently been explored as a relatively sustainable alternative. Very recently some studies have been carried out to pyramid different Bt-sourced cry genes and even other genes like the one encoding for phytase enzyme. Such technique is a relatively recent advancement and one of the most important novel genetic engineering tools of the time that is yet to go mainstream, despite its untapped potential. Therefore, in this chapter, we tried to compare different crop protection strategies and make a point that gene pyramiding could be a better alternative if not the only one for sustainable pest management.
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References
Abudulai M, Salifu AB, Opare-Atakora D, Haruna M, Denwar NN, Baba II (2012) Yield loss at the different growth stages in soybean due to insect pests in Ghana. Arch Phytopathol Plant Protect 45:1796–1809
Ahmed N, Saini J, Sharma R, Seth MJHJoAR (2017) Performance of chickpea under organic and inorganic sources of nutrients at different soil moisture regimes in chickpea-okra cropping system. Himachal J Agric Res 43:23–28
Allard RW, Allard RW (1999) Principles of plant breeding. Wiley, New York
Anderson SJ, Simmons HE, French-Monar RD, Munkvold GP (2016) Susceptibility of maize inbreds and incidence of symptomless infection by the head smut pathogen, sphacelotheca reilana. Plant Health Prog 17:1–5
Bahri B, Shah S, Hussain S, Leconte M, Enjalbert J, De Vallavieille-Pope C (2011) Genetic diversity of the wheat yellow rust population in Pakistan and its relationship with host resistance. Plant Pathol 60:649–660
Ben-Dov E (2014) Bacillus thuringiensis subsp. Israelensis and its dipteran-specific toxins. Toxins 6:1222–1243
Brévault T, Heuberger S, Zhang M, Ellers-Kirk C, Ni X, Masson L et al (2013) Potential shortfall of pyramided transgenic cotton for insect resistance management. Proc Natl Acad Sci 110:5806–5811
Campagne P, Kruger M, Pasquet R, Le Ru B, Van Den Berg J (2013) Dominant inheritance of field-evolved resistance to bt corn in busseola fusca. PLoS One 8:e69675
Cao J, Zhao J-Z, Tang J, Shelton A, Earle E (2002) Broccoli plants with pyramided cry1ac and cry1c bt genes control diamondback moths resistant to cry1a and cry1c proteins. Theor Appl Genet 105:258–264
Cao J, Shelton AM, Earle ED (2008) Sequential transformation to pyramid two bt genes in vegetable indian mustard (brassica juncea l.) and its potential for control of diamondback moth larvae. Plant Cell Rep 27:479
Chen H, Tang W, Xu C, Li X, Lin Y, Zhang Q (2005) Transgenic indica rice plants harboring a synthetic cry2a∗ gene of bacillus thuringiensis exhibit enhanced resistance against lepidopteran rice pests. Theor Appl Genet 111:1330
Chen H, Zhang G, Zhang Q, Lin Y (2008) Effect of transgenic bacillus thuringiensis rice lines on mortality and feeding behavior of rice stem borers (lepidoptera: Crambidae). J Econ Entomol 101:182–189
Cheng X, Sardana R, Kaplan H, Altosaar I (1998) Agrobacterium-transformed rice plants expressing synthetic cryia (b) and cryia (c) genes are highly toxic to striped stem borer and yellow stem borer. Proc Natl Acad Sci 95:2767–2772
Collinge DB, Lund OS, Thordal-Christensen H (2007) What are the prospects for genetically engineered, disease resistant plants? In: Sustainable disease management in a european context. Springer, Dordrecht
Crickmore N. (2017) Bacillus thuringiensis Toxin Classification. In: Fiuza L., Polanczyk R., Crickmore N. (eds) Bacillus thuringiensis and Lysinibacillus sphaericus. Springer, Cham
Danny J, Llewellyn M, Brown Y, Cousins Y, Hartweck D, Last A, et al (1992) The science behind transgenic cotton plants. In: Proceedings of 6th Australian cotton conference, Broadbeach and Queensland, Australia
Datta K, Baisakh N, Thet KM, Tu J, Datta S (2002) Pyramiding transgenes for multiple resistance in rice against bacterial blight, yellow stem borer and sheath blight. Theor Appl Genet 106:1–8
De Maagd RA, Bravo A, Crickmore N (2001) How bacillus thuringiensis has evolved specific toxins to colonize the insect world. Trends Genet 17:193–199
Dhurua S, Gujar GT (2011) Field-evolved resistance to bt toxin cry1ac in the pink bollworm, pectinophora gossypiella (saunders)(lepidoptera: Gelechiidae), from India. Pest Manag Sci 67:898–903
Ferré J, Van Rie J (2002) Biochemistry and genetics of insect resistance to b acillus thuringiensis. Annu Rev Entomol 47:501–533
Ferry N, Edwards MG, Gatehouse JA, Gatehouse AM (2004) Plant–insect interactions: molecular approaches to insect resistance. Curr Opin Biotechnol 15:155–161
Feto NA (2016) Bacillus spp. and their biotechnological roles in green industry. Bacilli and Agrobiotechnology. In: Islam MT, Rahman MM, Pandey P, Jha CK, Aeron A (eds) Bacilli and Agrobiotechnology. Springer Verlag. ISBN: 978-3-319-44408-6, pp 143–162. https://doi.org/10.1007/978-3-319-44409-3
Fukuoka S, Saka N, Mizukami Y, Koga H, Yamanouchi U, Yoshioka Y et al (2015) Gene pyramiding enhances durable blast disease resistance in rice. Sci Rep 5:7773
Gahan LJ, Ma Y-T, Macgregorcoble ML, Gould F, Moar WJ, Heckel DG (2005) Genetic basis of resistance to cry1ac and cry2aa in heliothis virescens (lepidoptera: Noctuidae). J Econ Entomol 98:1357–1368
Geffroy V, Sicard D, De Oliveira JC, Sévignac M, Cohen S, Gepts P et al (1999) Identification of an ancestral resistance gene cluster involved in the coevolution process between phaseolus vulgaris and its fungal pathogen colletotrichum lindemuthianum. Mol Plant-Microbe Interact 12:774–784
Graves JB, Leonard BR, Ottea J (1999) Chemical approaches to managing arthropod pests. In: Handbook of Pest Management. Marcel Dekker, Inc, New York, pp 449–486
Grisley W (1997) Crop-pest yield loss: a diagnostic study in the Kenya highlands. Int J Pest Manag 43:137–142
Ibrahim RA, Shawer DM (2014) Transgenic bt-plants and the future of crop protection (an overview). Int J Agric Food Res 3(1):14–40
Jackson R, Bradley J Jr, Van Duyn J (2004) Performance of feral and cry1ac-selected helicoverpa zea (lepidoptera: Noctuidae) strains on transgenic cottons expressing one or two bacillus thuringiensis ssp. Kurstaki proteins under greenhouse conditions. J Entomol Sci 39:46–55
Jain D, Sunda SD, Sanadhya S, Nath DJ, Khandelwal SK (2017) Molecular characterization and pcr-based screening of cry genes from bacillus thuringiensis strains. 3 Biotech 7:4
Jenkins JL, Dean DH (2000) Exploring the mechanism of action of insecticidal proteins by genetic engineering methods. In: Genetic engineering. Springer, Boston
Jiang G, Xu C, Tu J, Li X, He Y, Zhang Q (2004) Pyramiding of insect-and disease-resistance genes into an elite indica, cytoplasm male sterile restorer line of rice, ‘minghui 63′. Plant Breed 123:112–116
Jiang F, Zhang T, Bai S, Wang Z, He K (2016) Evaluation of Bt corn with pyramided genes on efficacy and insect resistance management for the Asian corn borer in China. PLoS One 11:e0168442
Jurat-Fuentes JL, Crickmore N (2017) Specificity determinants for cry insecticidal proteins: insights from their mode of action. J Invertebr Pathol 142:5–10
Keshavareddy G, Kumar A (2018) Characterization of bt transgenic plants: a review. Int J Curr Microbiol App Sci 7:3035–3051
Kouser S, Qaim M (2011) Impact of bt cotton on pesticide poisoning in smallholder agriculture: a panel data analysis. Ecol Econ 70:2105–2113
Kuddus M, Ahmad I (2013) Isolation of novel chitinolytic bacteria and production optimization of extracellular chitinase. J Genet Eng Biotechnol 11:39–46
Kumar S, Chandra A, Pandey K (2006) Genetic transformation of lucerne (medicago sativa l.) for weevil (hypera postica) resistance. In: Extended Summaries, national seminar transfiguration crops Indian agriculture: status, risks and acceptance, Hisar, India, pp 35–37
Kumar S, Chandra A, Pandey K (2008) Bacillus thuringiensis (bt) transgenic crop: an environment friendly insect-pest management strategy. J Environ Biol 29:641–653
Liu Y, Chen L, Liu Y, Dai H, He J, Kang H et al (2016) Marker assisted pyramiding of two brown planthopper resistance genes, bph3 and bph27 (t), into elite rice cultivars. Rice 9:27
Malav AK, Chandrawat I, Chandrawat KS (2016) Gene pyramiding: an overview. Int J Curr Res Biosci Plant Biol 3:22–28
Maqbool SB, Riazuddin S, Loc NT, Gatehouse AM, Gatehouse JA, Christou P (2001) Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests. Mol Breed 7:85–93
Maxmen A (2013) Crop pests: under attack. Nature 501:S15–S17
Meenakshi MA, Singh AK, Sanyal I, Altosaar I, Amla DV (2011) Pyramiding of modified cry1ab and cry1ac genes of bacillus thuringiensis in transgenic chickpea (cicer arietinum l.) for improved resistance to pod borer insect helicoverpa armigera. Euphytica 182:87
Mekonnen T, Haileselassie T, Tesfaye K (2017) Identification, mapping and pyramiding of genes/quantitative trait loci (qtls) for durable resistance of crops to biotic stresses. J Plant Pathol Microbiol 8:142
Meziadi C, Richard MM, Derquennes A, Thareau V, Blanchet S, Gratias A et al (2016) Development of molecular markers linked to disease resistance genes in common bean based on whole genome sequence. Plant Sci 242:351–357
Musser F, Catchot A Jr, Davis J, Herbert D Jr, Lorenz G, Reed T et al (2016) 2015 soybean insect losses in the southern us. Midsouth Entomologist 9:5–17
Neya BJ, Zida PE, Sereme, D, Lund OS, Traore O (2015) Evaluation of yield losses caused by cowpea aphid-borne mosaic virus (CABMV) in 21 cowpea (Vigna unguiculata (L.) Walp.) varieties in Burkina Faso. Pak J Biol Sci 18:304–313
Niu L, Mannakkara A, Qiu L, Wang X, Hua H, Lei C et al (2017) Transgenic bt rice lines producing cry1ac, cry2aa or cry1ca have no detrimental effects on brown planthopper and pond wolf spider. Sci Rep 7:1940
Oerke E-C (2006) Crop losses to pests. J Agric Sci 144:31–43
Okechukwu RU, Ekpo EJA, Okechukwu OC (2010) Seed to plant transmission of Xanthomonas campestris pv. vignicola isolates in cowpea. Afr J Agric Res 5(6):431–435
Osman G, Already R, Assaeedi A, Organji S, El-Ghareeb D, Abulreesh H, Althubiani A (2015) Bioinsecticide bacillus thuringiensis a comprehensive review. Egypt J Biol Pest Control 25:271
Palma L, Muñoz D, Berry C, Murillo J, Caballero P (2014) Bacillus thuringiensis toxins: an overview of their biocidal activity. Toxins 6:3296–3325
Pardo-Lopez L, Soberon M, Bravo A (2013) Bacillus thuringiensis insecticidal three-domain cry toxins: mode of action, insect resistance and consequences for crop protection. FEMS Microbiol Rev 37:3–22
Peralta C, Palma L (2017) Is the insect world overcoming the efficacy of bacillus thuringiensis? Toxins 9:39
Punja ZK (2006) Recent developments toward achieving fungal disease resistance in transgenic plants. Can J Plant Pathol 28:S298–S308
Riaz N, Husnain T, Fatima T, Makhdoom R, Bashir K, Masson L et al (2006) Development of indica basmati rice harboring two insecticidal genes for sustainable resistance against lepidopteran insects. S Afr J Bot 72:217–223
Roh JY, Choi JY, Li MS, Jin BR, Je YH (2007) Bacillus thuringiensis as a specific, safe, and effective tool for insect pest control. J Microbiol Biotechnol 17:547
Roh JY, Liu Q, Lee DW, Tao X, Wang Y, Shim HJ et al (2009) Bacillus thuringiensis serovar mogi (flagellar serotype 3a3b3d), a novel serogroup with a mosquitocidal activity. J Invertebr Pathol 102:266–268
Santos-Amaya OF, Rodrigues JV, Souza TC, Tavares CS, Campos SO, Guedes RN, Pereira EJ (2015) Resistance to dual-gene bt maize in spodoptera frugiperda: selection, inheritance, and cross-resistance to other transgenic events. Sci Rep 5:srep18243
Scheyer A, Graeff C, Morville S, Mirabel P, Millet M (2005) Analysis of some organochlorine pesticides in an urban atmosphere (Strasbourg, east of France). Chemosphere 58:1517–1524
Schnepf E, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J et al (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev 62:775–806
Soberon M, Monnerat R, Alejandra B (2016) Mode of action of cry toxins from bacillus thuringiensis and resistance mechanisms. In: Microbial toxins. Springer, Dordrecht, pp 1–13
Song X, Kain W, Cassidy D, Wang P (2015) Resistance to bacillus thuringiensis toxin cry2ab in trichoplusia ni is conferred by a novel genetic mechanism. Appl Environ Microbiol 81:5184–5195
Srivastava D, Shamim M, Kumar M, Mishra A, Pandey P, Kumar D et al (2017) Current status of conventional and molecular interventions for blast resistance in rice. Rice Sci 24:299–321
Summers R, Brown J (2013) Constraints on breeding for disease resistance in commercially competitive wheat cultivars. Plant Pathol 62:115–121
Tabashnik BE, Carrière Y (2010) Field-evolved resistance to bt cotton: bollworm in the us and pink bollworm in India. Southwest Entomol 35:417–424
Tu J, Zhang G, Datta K, Xu C, He Y, Zhang Q et al (2000) Field performance of transgenic elite commercial hybrid rice expressing bacillus thuringiensis δ-endotoxin. Nat Biotechnol 18:1101
Varshney RK, Graner A, Sorrells ME (2005) Genomics-assisted breeding for crop improvement. Trends Plant Sci 10:621–630
Vincelli P (2016) Genetic engineering and sustainable crop disease management: opportunities for case-by-case decision-making. Sustainability 8:495
Walker D, Boerma HR, All J, Parrott W (2002) Combining cry1Ac with QTL alleles from PI 229358 to improve soybean resistance to lepidopteran pests. Mol Breed 9:43–51
Wang YN, Ke KQ, Li YH, Han LZ, Liu YM, Hua HX, Peng YF (2016) Comparison of three transgenic bt rice lines for insecticidal protein expression and resistance against a target pest, chilo suppressalis (lepidoptera: Crambidae). Insect Sci 23:78–87
Xu C, Wang B-C, Yu Z, Sun M (2014) Structural insights into bacillus thuringiensis cry, cyt and parasporin toxins. Toxins 6:2732–2770
Yang Z, Chen H, Tang W, Hua H, Lin Y (2011) Development and characterisation of transgenic rice expressing two bacillus thuringiensis genes. Pest Manag Sci 67:414–422
Ye R, Huang H, Yang Z, Chen T, Liu L, Li X et al (2009) Development of insect-resistant transgenic rice with cry1c∗-free endosperm. Pest Manag Sci 65:1015–1020
Zheng X, Yang Y, Xu H, Chen H, Wang B, Lin Y, Lu Z (2011) Resistance performances of transgenic bt rice lines t2a-1 and t1c-19 against cnaphalocrocis medinalis (lepidoptera: Pyralidae). J Econ Entomol 104:1730–1735
Zhou Y, Choi Y-L, Sun M, Yu Z (2008) Novel roles of bacillus thuringiensis to control plant diseases. Appl Microbiol Biotechnol 80:563–572
Zhu X, Lei Y, Yang Y, Baxter SW, Li J, Wu Q et al (2015) Construction and characterisation of near-isogenic plutella xylostella (lepidoptera: Plutellidae) strains resistant to cry1ac toxin. Pest Manag Sci 71:225–233
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Segolela, J.C., Wokadala, O.C., Feto, N.A. (2019). Bacillus thuringiensis-Based Gene Pyramiding: a Way Forward for a Combined Horizontal and Vertical Resistance in Plant. In: Islam, M., Rahman, M., Pandey, P., Boehme, M., Haesaert, G. (eds) Bacilli and Agrobiotechnology: Phytostimulation and Biocontrol. Bacilli in Climate Resilient Agriculture and Bioprospecting. Springer, Cham. https://doi.org/10.1007/978-3-030-15175-1_15
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