Abstract
There has been a huge increase in our knowledge of nematophagous fungi, however, the methodology used to study these organisms are unique and have changed little over the years. Nematophagous fungi are easy to find and cultivate, but their peculiar mode of life makes it necessary to use certain techniques to bring them under observation. These methods are detailed in this chapter and include techniques for obtaining mixed cultures of the fungi from nature, for isolating taxa into pure culture, for observing living material and for making permanent microscope preparations.
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References
Ahrén, D., Ursing, B. M., & Tunlid, A. (1998). Phylogeny of nematode-trapping fungi based on 18 S rDNA sequences. FEMS Microbiology Letters, 158, 179–184.
Barron, G. L. (1969). Isolation and maintenance of endoparasitic nematophagous hyphomycetes. Canadian Journal of Botany, 47, 1899–1902.
Barron, G. L. (1977). The nematode-destroying fungi. Canadian Biological Publications Ltd.
Barron, G. L. (1978). Nematophagous fungi: Endoparasites of Rhabditis terricola. Microbial Ecology, 4, 157–163.
Barron, G. L. (1979). Observations on predatory fungi. Canadian Journal of Botany, 57, 187–193.
Bordallo, J., Lopez-Llorca, L., Jansson, H. B., Salinas, J., Persmark, L., & Asensio, L. (2002). Colonization of plant roots by egg-parasitic and nematode-trapping fungi. New Phytologist, 154, 491–499.
Brenner, S. (1974). The genetics of Caenorhabditis elegans. Genetics, 77, 71–94.
Capstick, C., Twinn, D., & Waid, J. (1957). Predation of natural populations of free-living nematodes by fungi 1). Nematologica, 2, 193–201.
Chen, T. H., Hsu, C. S., Tsai, P. J., Ho, Y. F., & Lin, N. S. (2001). Heterotrimeric G-protein and signal transduction in the nematode-trapping fungus Arthrobotrys dactyloides. Planta, 212, 858–863.
Connell, S. L., & Padgett, D. E. (1988). An improved technique for making permanent slide cultures of fungi. Mycopathologia, 101, 165–166.
Cooke, R. C. (1961). Agar disk method for the direct observation of nematode-trapping fungi in the soil. Nature, 191, 1411–1142.
Cooke, R. C. (1962). The ecology of nematode-trapping fungi in the soil. Annals of Applied Biology, 50, 507–513.
Cooke, R. C., & Godfrey, B. (1964). A key to the nematode-destroying fungi. Transactions of the British Mycological Society, 47, 61–74
Coolen, W. (1979). Methods for the extraction of Meloidogyne spp. and other nematodes from roots and soil. In F. Lamberti & C. E. Taylor (Eds.), Root-knot nematodes (Meloidogyne species). Systematics, biology and control (pp. 317–329). New York: Academic Press.
Correa, B., Purchio, A., Paula, C., & Gambale, W. (1986). Evaluation of a fluorescent method (fluorescein diacetate and ethidium bromide solution) in the study of the viability Cryptococcus neoformans strains. Mycopathologia, 96, 91–96.
Dackman, C., Olsson, S., Jansson, H. B., Lundgren, B., & Nordbring-Hertz, B. (1987). Quantification of predatory and endoparasitic nematophagous fungi in soil. Microbial Ecology, 13, 89–93.
Diehl, W. W. (1929). An improved method for sealing microscopic mounts. Science, 69, 276–277.
Dixon, S. M., & Duddington, C. L. (1951). Permanent preparation of fungi growing on agar. Nature, 168, 38–39.
Dong, L. Q., Mo, M. H., Yang, J. K., & Zhang, K. Q. (2007). A method for obtaining quantities of Caenorhabditis elegans eggs. Nematology, 9, 743–744.
Drechsler, C. (1937). Some hyphomycetes that prey on free-living terricolous nematodes. Mycologia, 29, 447–552.
Drechsler, C. (1941). Predacious fungi. Biological Reviews of the Cambridge Philosophical Society, 16, 265–290.
Duddington, C. (1955). Notes on the technique of handling predacious fungi. Transactions of the British Mycological Society, 38, 97–103
Duddington, C., & Dixon, S. M. (1951). Permanent preparations of fungi growing on agar. Nature, 168, 38–39.
Duddington, C., & Dixon, S. M. (1953). Some methods for making permanent preparations of micro-fungi. The Journal of the Quekett Microscopical Club, 3, 305
Eren, J., & Pramer, D. (1965). The most probable number of nematode-trapping fungi in soil. Soil Science, 99, 285.
Erni, R., Rossell, M. D., Kisielowski, C., & Dahmen, U. (2009). Atomic-resolution imaging with a sub-50-pm electron probe. Physical Review Letters, 102, 96101.
Farrell, F., Jaffee, B., & Strong, D. (2006). The nematode-trapping fungus Arthrobotrys oligospora in soilof the Bodega marine reserve: Distribution and dependenceon nematode-parasitized moth larvae. Soil Biology and Biochemistry, 38, 1422–1429.
Feder, W. A., Everard, C., & Wootton, L. (1963). Sensitivity of several species of the nematophagous fungus Dactylella to a morphogenic substance derived from free-living nematodes. Nematologica, 9, 49–54.
Foster, L. M., Kozak, K. R., Loftus, M. G., Stevens, J. J., & Ross, I. K. (1993). The polymerase chain reaction and its application to filamentous fungi. Mycological Research, 97, 769–781.
Fowler, M. (1970). New Zealand predaceous fungi. New Zealand Journal of Botany, 8, 283–302.
Giuma, A., & Cooke, R. (1972). Some endozoic fungi parasitic on soil nematodes. Transactions of the British Mycological Society, 59, 213–218
Gray, N. (1983). Ecology of nematophagous fungi: Panagrellus redivivus as the target organism. Plant and Soil, 73, 293–297.
Gray, N. (1984a). Ecology of nematophagous fungi: Comparison of the soil sprinkling method with the Baermann funnel technique in the isolation of endoparasites. Soil Biology and Biochemistry, 16, 81–83.
Gray, N. (1984b). Ecology of nematophagous fungi: Methods of collection, isolation and maintenance of predatory and endoparasitic fungi. Mycopathologia, 86, 143–153.
Gray, N. (1984c). The effect of fungal parasitism and predation on the population dynamics of nematodes in the activated sludge process. Annals of Applied Biology, 104, 143–149.
Gray, N. (1985). Ecology of nematophagous fungi: Effect of soil moisture, organic matter, pH and nematode density on distribution. Soil Biology and Biochemistry, 17, 499–507.
Gray, N. (1987). Nematophagous fungi with particular reference to their ecology. Biological Reviews, 62, 245–304.
Gray, N. (1988). Ecology of nematophagous fungi: Effect of the soil nutrients N, P and K, and seven major metals on distribution. Plant and Soil, 108, 286–290.
Gray, N., & Bailey, F. (1985). Ecology of nematophagous fungi: Vertical distribution in a deciduous woodland. Plant and Soil, 86, 217–223.
Hagedorn, G., & Scholler, M. (1999). A reevaluation of predatory orbiliaceous fungi. I. Phylogenetic analysis using rDNA sequence data. Sydowia, 51, 27–48.
Hao, Y., Mo, M., Su, H., & Zhang, K. Q. (2005). Ecology of aquatic nematode-trapping hyphomycetes in southwestern China. Aquatic Microbial Ecology, 40, 175–181.
Hawkswell, A. (2001). Iodine-lactophenol as a mycological mounting medium. Field Mycology, 2, 12.
Hawksworth, D. L. (2012). Managing and coping with names of pleomorphic fungi in a period of transition. IMA Fungus 3, 15–24.
Heintz, C. E. (1978). Assessing the predacity of nematode-trapping fungi in vitro. Mycologia, 70, 1086–1100
Heintz, C., & Pramer, D. (1972). Ultrastructure of nematode-trapping fungi. Journal of Bacteriology, 110, 1163–1170.
Herrera-Medina, M. J., Steinkellner, S., Vierheilig, H., Ocampo Bote, J. A., & Garrido, J. M. G. (2007). Abscisic acid determines arbuscule development and functionality in the tomato arbuscular mycorrhiza. New Phytologist, 175, 554–564.
Higgins, M., & Pramer, D. (1967). Fungal morphogenesis: Ring formation and closure by Arthrobotrys dactyloides. Science, 155, 345–346.
Hu, W., Li, Y., Mo, M., & Zhang, K. Q. (2006). A new nematode-trapping hyphomycete of Arthrobotrys. Mycotaxon, 95, 181–184.
Jaffee, B., & Strong, D. (2005). Strong bottom-up and weak top-down effects in soil: Nematode-parasitized insects and nematode-trapping fungi. Soil Biology and Biochemistry, 37, 1011–1021.
Jaffee, B., Strong, D., & Muldoon, A. (1996). Nematode-trapping fungi of a natural shrubland: Tests for food chain involvement. Mycologia, 88, 554–564.
Jansson, H. B. (1994). Adhesion of conidia of Drechmeria coniospora to Caenorhabditis elegans wild type and mutants. Journal of Nematology, 26, 430–435.
Janssona, H. B. (1982). Attraction of nematodes to endoparasitic nematophagous fungi. Transactions of the British Mycological Society, 79, 25–29.
Jensen, C. (1994). Fluoreszenzmikroskopische Möglichkeiten zur in situ-Beobachtung nematophager Pilze im Boden. Dissertationes Botanicae, 217. Berlin: Cramer.
Jensen, C., & Lysek, G. (1991). Direct observation of trapping activities of nematode-destroying fungi in the soil using fluorescence microscopy. FEMS Microbiology Letters, 85, 207–210.
Jensen, C., & Lysek, G. (1995). Fluorescence microscopy of fungi in native soil-improvement by additional substances. Microscopy and Analysis, 49, 7–9.
Jensen, C. H., Neumeister, H., & Lysek, G. (1997). Nematophagous fungi-study by fluorescence microscopy and edx-technique of the periodicity of trap formation in soil. Biological Rhythm Research, 28, 365–373.
Jensen, C., Neumeister-Kemp, H., & Lysek, G. (1998). Fluorescence microscopy for the observation of nematophagous fungi inside soil. Mycologist, 12, 107–111.
Kohlmeyer, J., & Kohlmeyer, E. (1972). Permanent microscopic mounts. Mycologia, 64, 666–669.
Lambert, M., Kremer, S., & Anke, H. (1995). Antimicrobial, phytotoxic, nematicidal, cytotoxic, and mutagenic activities of 1-hydroxypyrene, the initial metabolite in pyrene metabolism by the basidiomycete Crinipellis stipitaria. Bulletin of Environmental Contamination and Toxicology, 55, 251–257.
Leck, A. (1999). Preparation of lactophenol cotton blue slide mounts. Community Eye Health, 12, 24.
Li, Y., Hyde, K. D., Jeewon, R., Cai, L., Vijaykrishna, D., & Zhang, K. Q. (2005). Phylogenetics and evolution of nematode-trapping fungi (Orbiliales) estimated from nuclear and protein coding genes. Mycologia, 97, 1034–1046.
Li, Y., Jeewon, R., Hyde, K. D., Mo, M. H., & Zhang, K. Q. (2006). Two new species of nematode-trapping fungi: Relationships inferred from morphology, rDNA and protein gene sequence analyses. Mycological Research, 110, 790–800.
Li, J., Yang, J., Liang, L., & Zhang, K. Q. (2008). Taxonomic revision of the nematode-trapping fungus Arthrobotrys multisecundaria. Journal of Microbiology, 46, 513–518.
Liou, G. Y., & Tzean, S. S. (1997). Phylogeny of the genus Arthrobotrys and allied nematode-trapping fungi based on rDNA sequences. Mycologia, 89, 876–884
Lopez-Llorca, L., & Duncan, J. (1986). New media for the estimation of fungal infection in eggs of the cereal cyst nematode. Nematologica (Netherlands), 32, 486–490.
Lysek, G., & Nordbring-Hertz, B. (1983). Die Biologie nematodenfangender Pilze. Forum Mikrobiologie. Darmstadt, 6, 201–208.
Mitsui, Y. (1985). Distribution and ecology of nematode-trapping fungi in Japan. JARQ, 18, 182–193.
Neumeister, H., Moritz, M., Schleibinger, H., & Martiny, H. (1996). Investigation of allergic potential induced by fungi on air filters of HVAC systems. Proceedings of Indoor Air ’96, Nagoya, Japan.
Nordbring-Hertz, B. (1972). Scanning electron microscopy of the nematode-trapping organs in Arthrobotrys oligospora. Physiologia Plantarum, 26, 279–284.
Nordbring-Hertz, B. (1984). Carbohydrate interactions in nematode-trapping fungi. In DH Jennings & ADM Rayner (Eds.), The ecology and physiology of the fungal mycelium (pp. 419–432). Cambridge: Cambridge University Press.
Park, J., Gams, W., Scholler, M., Ghisalberti, E., & Sivasithamparam, K. (2002). Orbiliaceous nematode-trapping fungi and related species in Western Australia and their biological activities. Australasian Mycologist, 21, 45–52.
Persmark, L., & Jansson, H. B. (1997). Nematophagous fungi in the rhizosphere of agricultural crops. FEMS Microbiology Ecology, 22, 303–312.
Persson, C., Olsson, S., & Jansson, H. B. (2000). Growth of Arthrobotrys superba from a birch wood resource base into soil determined by radioactive tracing. FEMS Microbiology Ecology, 31, 47–51.
Pfister, D. H., (1997). Castor, Pollux and life histories of fungi. Mycologia, 89(1), 1–23
Pramer, D., & Stoll, N. R. (1959). Nemin: A morphogenic substance causing trap formation by predaceous fungi. Science, 129, 966–967.
Rubner, A. (1996). Revision of predacious hyphomycetes in the Dactylella-Monacrosporium complex. Studies in Mycology, 39, 1–134.
Ryan, M., Smith, D., & Jeffries, P. (2000). A decision-based key to determine the most appropriate protocol for the preservation of fungi. World Journal of Microbiology and Biotechnology, 16, 183–186.
Saikawa, M., & Morikawa, C. (1985). Electron microscopy on a nematode-trapping fungus, Acaulopage pectospora. Canadian Journal of Botany, 63, 1386–1390.
Saxena, G., & Lysek, G. (1993). Observation of nematophagous fungi in natural soils by fluorescence microscopy and their correlation with isolation. Mycological Research, 97, 1005–1011.
Schindler, A. (1961). A simple substitute for a Baermann funnel. Plant Disease Reporter, 45, 747–748.
Scholler, M., Hagedorn, G., & Rubner, A. (1999). A reevaluation of predatory orbiliaceous fungi. II. A new generic concept. Sydowia, 51, 89–113.
Schwarz, M., Köpcke, B., Weber, R. W. S., Sterner, O., & Anke, H. (2004). 3-Hydroxypropionic acid as a nematicidal principle in endophytic fungi. Phytochemistry, 65, 2239–2245.
Sharma, V., Walia, S., Kumar, J., Nair, M. G., & Parmar, B. S. (2003). An efficient method for the purification and characterization of nematicidal azadirachtins A, B, and H, using MPLC and ESIMS. Journal of Agricultural and Food Chemistry, 51, 3966–3972.
Shepherd, A. M. (1955). Some observations on the distribution and biology of fungi predaceous on nematodes. Ph. D. Thesis, University of London.
Soderstrom, B., & Erland, S. (1986). Isolation of fluorescein diacetate stained hyphae from soil by micromanipulation. Transactions of the British Mycological Society, 86, 465–468.
Southey, J. F. (1986). Laboratory methods for work with plant and soil nematodes. HMSO Books.
Staniland, L. (1954). A modification of the Baermann funnel technique for the collection of nematodes from plant material. Journal of Helminthology, 28, 115–118.
Stirling, G., Wilson, E., Stirling, A., Pankhurst, C., Moody, P., Bell, M., & Halpin, N. (2005). Amendments of sugarcane trash induce suppressiveness to plant-parasitic nematodes in a sugarcane soil. Australasian Plant Pathology, 34, 203–211.
Stock, S. P., & Nadler, S. A. (2006). Morphological and molecular characterisation of Panagrellus spp. (Cephalobina: Panagrolaimidae): Taxonomic status and phylogenetic relationships. Nematology, 8, 921–938.
Tunlid, A., Johansson, T., & Nordbring-Hertz, B. (1991). Surface polymers of the nematode-trapping fungus Arthrobotrys oligospora. Journal of General Microbiology, 137, 1231–1240.
Vaitkevicius, K., Lindmark, B., Ou, G., Song, T., Toma, C., Iwanaga, M., Zhu, J., Andersson, A., Hammarström, M. L., Wai, S. N., Tuck, S. (2006). A Vibrio cholerae protease needed for killing of Caenorhabditis elegans has a role in protection from natural predator grazing. Proceedings of the National Academy of Sciences, 103, 9280–9285.
Volkmann-Kohlmeyer, B., & Kohlmeyer, J. (1996). How to prepare truly permanent microscope slides. Mycologist, 10, 107–108.
Von Sengbusch, P., Hechler, J., & Müller, U. (1983). Molecular architecture of fungal cell walls. An approach by use of fluorescent markers. European Journal of Cell Biology, 30, 305–312.
Wang, J., & Higgins, V. J. (2005). Nitric oxide has a regulatory effect in the germination of conidia of Colletotrichum coccodes. Fungal Genetics and Biology, 42, 284–292.
White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: a guide to methods and applications. (Innis, M. A., Gelfand, D. H., Sninsky, J. J., White, T. J., eds). Academic Press, New York, USA: 315–322.
Whitehead, A., & Hemming, J. (1965). A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology, 55, 25–38.
Wilchek, M., & Bayer, E. (1990). Methods in enzymology. San Diego: Academic Press Inc.
Wood, F. (1973). Nematode-trapping fungi from a tussock grassland soil in New Zealand. New Zealand Journal of Botany, 11, 231–240.
Wyborn, C., Priest, D., & Duddington, C. (1969). Selective technique for the determination of nematophagous fungi in soils. Soil Biology and Biochemistry, 1, 101–102.
Xu, L. L., Lai, Y. L., Wang, L., & Liu, X. Z. (2011). Effects of abscisic acid and nitric oxide on trap formation and trapping of nematodes by the fungus Drechslerella stenobrocha AS6.1 Fungal Biology, 2 (114), 97–101.
Yang, Y., Yang, E., An, Z., & Liu, X. (2007) Evolution of nematode-trapping cells of predatory fungi of the Orbiliaceae based on evidence from rRNA-encoding DNA and multiprotein sequences. Proceedings of the National Academy of Sciences, USA, 104, 83–79.
Zhang, J., Mo, M., Deng, J., Liu, X. F., Bi, T. J., & Zhang, K. Q. (2005). Dactylella zhongdianensis sp. nov, a new predacious antagonist of nematodes. Mycotaxon, 92, 289–294.
Zuckerman, B., Matheny, M., & Acosta, N. (1994). Control of plant-parasitic nematodes by a nematicidal strain of Aspergillus niger. Journal of Chemical Ecology, 20, 33–43.
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Li, J., Hyde, K., Zhang, KQ. (2014). Methodology for Studying Nematophagous Fungi. In: Zhang, KQ., Hyde, K. (eds) Nematode-Trapping Fungi. Fungal Diversity Research Series, vol 23. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-8730-7_2
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