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Methodology for Studying Nematophagous Fungi

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Nematode-Trapping Fungi

Part of the book series: Fungal Diversity Research Series ((FDRS,volume 23))

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Abstract

There has been a huge increase in our knowledge of nematophagous fungi, however, the methodology used to study these organisms are unique and have changed little over the years. Nematophagous fungi are easy to find and cultivate, but their peculiar mode of life makes it necessary to use certain techniques to bring them under observation. These methods are detailed in this chapter and include techniques for obtaining mixed cultures of the fungi from nature, for isolating taxa into pure culture, for observing living material and for making permanent microscope preparations.

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References

  • Ahrén, D., Ursing, B. M., & Tunlid, A. (1998). Phylogeny of nematode-trapping fungi based on 18 S rDNA sequences. FEMS Microbiology Letters, 158, 179–184.

    Article  PubMed  Google Scholar 

  • Barron, G. L. (1969). Isolation and maintenance of endoparasitic nematophagous hyphomycetes. Canadian Journal of Botany, 47, 1899–1902.

    Article  Google Scholar 

  • Barron, G. L. (1977). The nematode-destroying fungi. Canadian Biological Publications Ltd.

    Google Scholar 

  • Barron, G. L. (1978). Nematophagous fungi: Endoparasites of Rhabditis terricola. Microbial Ecology, 4, 157–163.

    Article  Google Scholar 

  • Barron, G. L. (1979). Observations on predatory fungi. Canadian Journal of Botany, 57, 187–193.

    Article  Google Scholar 

  • Bordallo, J., Lopez-Llorca, L., Jansson, H. B., Salinas, J., Persmark, L., & Asensio, L. (2002). Colonization of plant roots by egg-parasitic and nematode-trapping fungi. New Phytologist, 154, 491–499.

    Article  Google Scholar 

  • Brenner, S. (1974). The genetics of Caenorhabditis elegans. Genetics, 77, 71–94.

    PubMed Central  PubMed  CAS  Google Scholar 

  • Capstick, C., Twinn, D., & Waid, J. (1957). Predation of natural populations of free-living nematodes by fungi 1). Nematologica, 2, 193–201.

    Article  Google Scholar 

  • Chen, T. H., Hsu, C. S., Tsai, P. J., Ho, Y. F., & Lin, N. S. (2001). Heterotrimeric G-protein and signal transduction in the nematode-trapping fungus Arthrobotrys dactyloides. Planta, 212, 858–863.

    Article  PubMed  CAS  Google Scholar 

  • Connell, S. L., & Padgett, D. E. (1988). An improved technique for making permanent slide cultures of fungi. Mycopathologia, 101, 165–166.

    Article  PubMed  CAS  Google Scholar 

  • Cooke, R. C. (1961). Agar disk method for the direct observation of nematode-trapping fungi in the soil. Nature, 191, 1411–1142.

    Article  PubMed  CAS  Google Scholar 

  • Cooke, R. C. (1962). The ecology of nematode-trapping fungi in the soil. Annals of Applied Biology, 50, 507–513.

    Article  Google Scholar 

  • Cooke, R. C., & Godfrey, B. (1964). A key to the nematode-destroying fungi. Transactions of the British Mycological Society, 47, 61–74

    Google Scholar 

  • Coolen, W. (1979). Methods for the extraction of Meloidogyne spp. and other nematodes from roots and soil. In F. Lamberti & C. E. Taylor (Eds.), Root-knot nematodes (Meloidogyne species). Systematics, biology and control (pp. 317–329). New York: Academic Press.

    Google Scholar 

  • Correa, B., Purchio, A., Paula, C., & Gambale, W. (1986). Evaluation of a fluorescent method (fluorescein diacetate and ethidium bromide solution) in the study of the viability Cryptococcus neoformans strains. Mycopathologia, 96, 91–96.

    Article  PubMed  CAS  Google Scholar 

  • Dackman, C., Olsson, S., Jansson, H. B., Lundgren, B., & Nordbring-Hertz, B. (1987). Quantification of predatory and endoparasitic nematophagous fungi in soil. Microbial Ecology, 13, 89–93.

    Article  PubMed  CAS  Google Scholar 

  • Diehl, W. W. (1929). An improved method for sealing microscopic mounts. Science, 69, 276–277.

    Article  PubMed  CAS  Google Scholar 

  • Dixon, S. M., & Duddington, C. L. (1951). Permanent preparation of fungi growing on agar. Nature, 168, 38–39.

    PubMed  Google Scholar 

  • Dong, L. Q., Mo, M. H., Yang, J. K., & Zhang, K. Q. (2007). A method for obtaining quantities of Caenorhabditis elegans eggs. Nematology, 9, 743–744.

    Article  Google Scholar 

  • Drechsler, C. (1937). Some hyphomycetes that prey on free-living terricolous nematodes. Mycologia, 29, 447–552.

    Article  Google Scholar 

  • Drechsler, C. (1941). Predacious fungi. Biological Reviews of the Cambridge Philosophical Society, 16, 265–290.

    Article  Google Scholar 

  • Duddington, C. (1955). Notes on the technique of handling predacious fungi. Transactions of the British Mycological Society, 38, 97–103

    Google Scholar 

  • Duddington, C., & Dixon, S. M. (1951). Permanent preparations of fungi growing on agar. Nature, 168, 38–39.

    Article  PubMed  CAS  Google Scholar 

  • Duddington, C., & Dixon, S. M. (1953). Some methods for making permanent preparations of micro-fungi. The Journal of the Quekett Microscopical Club, 3, 305

    Google Scholar 

  • Eren, J., & Pramer, D. (1965). The most probable number of nematode-trapping fungi in soil. Soil Science, 99, 285.

    Article  Google Scholar 

  • Erni, R., Rossell, M. D., Kisielowski, C., & Dahmen, U. (2009). Atomic-resolution imaging with a sub-50-pm electron probe. Physical Review Letters, 102, 96101.

    Article  CAS  Google Scholar 

  • Farrell, F., Jaffee, B., & Strong, D. (2006). The nematode-trapping fungus Arthrobotrys oligospora in soilof the Bodega marine reserve: Distribution and dependenceon nematode-parasitized moth larvae. Soil Biology and Biochemistry, 38, 1422–1429.

    Article  CAS  Google Scholar 

  • Feder, W. A., Everard, C., & Wootton, L. (1963). Sensitivity of several species of the nematophagous fungus Dactylella to a morphogenic substance derived from free-living nematodes. Nematologica, 9, 49–54.

    Article  Google Scholar 

  • Foster, L. M., Kozak, K. R., Loftus, M. G., Stevens, J. J., & Ross, I. K. (1993). The polymerase chain reaction and its application to filamentous fungi. Mycological Research, 97, 769–781.

    Article  CAS  Google Scholar 

  • Fowler, M. (1970). New Zealand predaceous fungi. New Zealand Journal of Botany, 8, 283–302.

    Article  Google Scholar 

  • Giuma, A., & Cooke, R. (1972). Some endozoic fungi parasitic on soil nematodes. Transactions of the British Mycological Society, 59, 213–218

    Google Scholar 

  • Gray, N. (1983). Ecology of nematophagous fungi: Panagrellus redivivus as the target organism. Plant and Soil, 73, 293–297.

    Article  Google Scholar 

  • Gray, N. (1984a). Ecology of nematophagous fungi: Comparison of the soil sprinkling method with the Baermann funnel technique in the isolation of endoparasites. Soil Biology and Biochemistry, 16, 81–83.

    Article  Google Scholar 

  • Gray, N. (1984b). Ecology of nematophagous fungi: Methods of collection, isolation and maintenance of predatory and endoparasitic fungi. Mycopathologia, 86, 143–153.

    Article  Google Scholar 

  • Gray, N. (1984c). The effect of fungal parasitism and predation on the population dynamics of nematodes in the activated sludge process. Annals of Applied Biology, 104, 143–149.

    Article  Google Scholar 

  • Gray, N. (1985). Ecology of nematophagous fungi: Effect of soil moisture, organic matter, pH and nematode density on distribution. Soil Biology and Biochemistry, 17, 499–507.

    Article  Google Scholar 

  • Gray, N. (1987). Nematophagous fungi with particular reference to their ecology. Biological Reviews, 62, 245–304.

    Article  Google Scholar 

  • Gray, N. (1988). Ecology of nematophagous fungi: Effect of the soil nutrients N, P and K, and seven major metals on distribution. Plant and Soil, 108, 286–290.

    Article  CAS  Google Scholar 

  • Gray, N., & Bailey, F. (1985). Ecology of nematophagous fungi: Vertical distribution in a deciduous woodland. Plant and Soil, 86, 217–223.

    Article  Google Scholar 

  • Hagedorn, G., & Scholler, M. (1999). A reevaluation of predatory orbiliaceous fungi. I. Phylogenetic analysis using rDNA sequence data. Sydowia, 51, 27–48.

    Google Scholar 

  • Hao, Y., Mo, M., Su, H., & Zhang, K. Q. (2005). Ecology of aquatic nematode-trapping hyphomycetes in southwestern China. Aquatic Microbial Ecology, 40, 175–181.

    Article  Google Scholar 

  • Hawkswell, A. (2001). Iodine-lactophenol as a mycological mounting medium. Field Mycology, 2, 12.

    Article  Google Scholar 

  • Hawksworth, D. L. (2012). Managing and coping with names of pleomorphic fungi in a period of transition. IMA Fungus 3, 15–24.

    Google Scholar 

  • Heintz, C. E. (1978). Assessing the predacity of nematode-trapping fungi in vitro. Mycologia, 70, 1086–1100

    Google Scholar 

  • Heintz, C., & Pramer, D. (1972). Ultrastructure of nematode-trapping fungi. Journal of Bacteriology, 110, 1163–1170.

    PubMed Central  PubMed  CAS  Google Scholar 

  • Herrera-Medina, M. J., Steinkellner, S., Vierheilig, H., Ocampo Bote, J. A., & Garrido, J. M. G. (2007). Abscisic acid determines arbuscule development and functionality in the tomato arbuscular mycorrhiza. New Phytologist, 175, 554–564.

    Article  PubMed  CAS  Google Scholar 

  • Higgins, M., & Pramer, D. (1967). Fungal morphogenesis: Ring formation and closure by Arthrobotrys dactyloides. Science, 155, 345–346.

    Article  PubMed  CAS  Google Scholar 

  • Hu, W., Li, Y., Mo, M., & Zhang, K. Q. (2006). A new nematode-trapping hyphomycete of Arthrobotrys. Mycotaxon, 95, 181–184.

    Google Scholar 

  • Jaffee, B., & Strong, D. (2005). Strong bottom-up and weak top-down effects in soil: Nematode-parasitized insects and nematode-trapping fungi. Soil Biology and Biochemistry, 37, 1011–1021.

    Article  CAS  Google Scholar 

  • Jaffee, B., Strong, D., & Muldoon, A. (1996). Nematode-trapping fungi of a natural shrubland: Tests for food chain involvement. Mycologia, 88, 554–564.

    Article  Google Scholar 

  • Jansson, H. B. (1994). Adhesion of conidia of Drechmeria coniospora to Caenorhabditis elegans wild type and mutants. Journal of Nematology, 26, 430–435.

    PubMed Central  PubMed  CAS  Google Scholar 

  • Janssona, H. B. (1982). Attraction of nematodes to endoparasitic nematophagous fungi. Transactions of the British Mycological Society, 79, 25–29.

    Google Scholar 

  • Jensen, C. (1994). Fluoreszenzmikroskopische Möglichkeiten zur in situ-Beobachtung nematophager Pilze im Boden. Dissertationes Botanicae, 217. Berlin: Cramer.

    Google Scholar 

  • Jensen, C., & Lysek, G. (1991). Direct observation of trapping activities of nematode-destroying fungi in the soil using fluorescence microscopy. FEMS Microbiology Letters, 85, 207–210.

    Article  Google Scholar 

  • Jensen, C., & Lysek, G. (1995). Fluorescence microscopy of fungi in native soil-improvement by additional substances. Microscopy and Analysis, 49, 7–9.

    Google Scholar 

  • Jensen, C. H., Neumeister, H., & Lysek, G. (1997). Nematophagous fungi-study by fluorescence microscopy and edx-technique of the periodicity of trap formation in soil. Biological Rhythm Research, 28, 365–373.

    Article  Google Scholar 

  • Jensen, C., Neumeister-Kemp, H., & Lysek, G. (1998). Fluorescence microscopy for the observation of nematophagous fungi inside soil. Mycologist, 12, 107–111.

    Article  Google Scholar 

  • Kohlmeyer, J., & Kohlmeyer, E. (1972). Permanent microscopic mounts. Mycologia, 64, 666–669.

    Article  Google Scholar 

  • Lambert, M., Kremer, S., & Anke, H. (1995). Antimicrobial, phytotoxic, nematicidal, cytotoxic, and mutagenic activities of 1-hydroxypyrene, the initial metabolite in pyrene metabolism by the basidiomycete Crinipellis stipitaria. Bulletin of Environmental Contamination and Toxicology, 55, 251–257.

    Article  PubMed  CAS  Google Scholar 

  • Leck, A. (1999). Preparation of lactophenol cotton blue slide mounts. Community Eye Health, 12, 24.

    PubMed Central  PubMed  CAS  Google Scholar 

  • Li, Y., Hyde, K. D., Jeewon, R., Cai, L., Vijaykrishna, D., & Zhang, K. Q. (2005). Phylogenetics and evolution of nematode-trapping fungi (Orbiliales) estimated from nuclear and protein coding genes. Mycologia, 97, 1034–1046.

    Article  PubMed  CAS  Google Scholar 

  • Li, Y., Jeewon, R., Hyde, K. D., Mo, M. H., & Zhang, K. Q. (2006). Two new species of nematode-trapping fungi: Relationships inferred from morphology, rDNA and protein gene sequence analyses. Mycological Research, 110, 790–800.

    Article  PubMed  CAS  Google Scholar 

  • Li, J., Yang, J., Liang, L., & Zhang, K. Q. (2008). Taxonomic revision of the nematode-trapping fungus Arthrobotrys multisecundaria. Journal of Microbiology, 46, 513–518.

    Article  CAS  Google Scholar 

  • Liou, G. Y., & Tzean, S. S. (1997). Phylogeny of the genus Arthrobotrys and allied nematode-trapping fungi based on rDNA sequences. Mycologia, 89, 876–884

    Google Scholar 

  • Lopez-Llorca, L., & Duncan, J. (1986). New media for the estimation of fungal infection in eggs of the cereal cyst nematode. Nematologica (Netherlands), 32, 486–490.

    Article  Google Scholar 

  • Lysek, G., & Nordbring-Hertz, B. (1983). Die Biologie nematodenfangender Pilze. Forum Mikrobiologie. Darmstadt, 6, 201–208.

    Google Scholar 

  • Mitsui, Y. (1985). Distribution and ecology of nematode-trapping fungi in Japan. JARQ, 18, 182–193.

    Google Scholar 

  • Neumeister, H., Moritz, M., Schleibinger, H., & Martiny, H. (1996). Investigation of allergic potential induced by fungi on air filters of HVAC systems. Proceedings of Indoor Air ’96, Nagoya, Japan.

    Google Scholar 

  • Nordbring-Hertz, B. (1972). Scanning electron microscopy of the nematode-trapping organs in Arthrobotrys oligospora. Physiologia Plantarum, 26, 279–284.

    Article  Google Scholar 

  • Nordbring-Hertz, B. (1984). Carbohydrate interactions in nematode-trapping fungi. In DH Jennings & ADM Rayner (Eds.), The ecology and physiology of the fungal mycelium (pp. 419–432). Cambridge: Cambridge University Press.

    Google Scholar 

  • Park, J., Gams, W., Scholler, M., Ghisalberti, E., & Sivasithamparam, K. (2002). Orbiliaceous nematode-trapping fungi and related species in Western Australia and their biological activities. Australasian Mycologist, 21, 45–52.

    Google Scholar 

  • Persmark, L., & Jansson, H. B. (1997). Nematophagous fungi in the rhizosphere of agricultural crops. FEMS Microbiology Ecology, 22, 303–312.

    Article  CAS  Google Scholar 

  • Persson, C., Olsson, S., & Jansson, H. B. (2000). Growth of Arthrobotrys superba from a birch wood resource base into soil determined by radioactive tracing. FEMS Microbiology Ecology, 31, 47–51.

    Article  PubMed  CAS  Google Scholar 

  • Pfister, D. H., (1997). Castor, Pollux and life histories of fungi. Mycologia, 89(1), 1–23

    Google Scholar 

  • Pramer, D., & Stoll, N. R. (1959). Nemin: A morphogenic substance causing trap formation by predaceous fungi. Science, 129, 966–967.

    Article  PubMed  CAS  Google Scholar 

  • Rubner, A. (1996). Revision of predacious hyphomycetes in the Dactylella-Monacrosporium complex. Studies in Mycology, 39, 1–134.

    Google Scholar 

  • Ryan, M., Smith, D., & Jeffries, P. (2000). A decision-based key to determine the most appropriate protocol for the preservation of fungi. World Journal of Microbiology and Biotechnology, 16, 183–186.

    Article  Google Scholar 

  • Saikawa, M., & Morikawa, C. (1985). Electron microscopy on a nematode-trapping fungus, Acaulopage pectospora. Canadian Journal of Botany, 63, 1386–1390.

    Article  Google Scholar 

  • Saxena, G., & Lysek, G. (1993). Observation of nematophagous fungi in natural soils by fluorescence microscopy and their correlation with isolation. Mycological Research, 97, 1005–1011.

    Article  Google Scholar 

  • Schindler, A. (1961). A simple substitute for a Baermann funnel. Plant Disease Reporter, 45, 747–748.

    Google Scholar 

  • Scholler, M., Hagedorn, G., & Rubner, A. (1999). A reevaluation of predatory orbiliaceous fungi. II. A new generic concept. Sydowia, 51, 89–113.

    Google Scholar 

  • Schwarz, M., Köpcke, B., Weber, R. W. S., Sterner, O., & Anke, H. (2004). 3-Hydroxypropionic acid as a nematicidal principle in endophytic fungi. Phytochemistry, 65, 2239–2245.

    Article  PubMed  CAS  Google Scholar 

  • Sharma, V., Walia, S., Kumar, J., Nair, M. G., & Parmar, B. S. (2003). An efficient method for the purification and characterization of nematicidal azadirachtins A, B, and H, using MPLC and ESIMS. Journal of Agricultural and Food Chemistry, 51, 3966–3972.

    Article  PubMed  CAS  Google Scholar 

  • Shepherd, A. M. (1955). Some observations on the distribution and biology of fungi predaceous on nematodes. Ph. D. Thesis, University of London.

    Google Scholar 

  • Soderstrom, B., & Erland, S. (1986). Isolation of fluorescein diacetate stained hyphae from soil by micromanipulation. Transactions of the British Mycological Society, 86, 465–468.

    Google Scholar 

  • Southey, J. F. (1986). Laboratory methods for work with plant and soil nematodes. HMSO Books.

    Google Scholar 

  • Staniland, L. (1954). A modification of the Baermann funnel technique for the collection of nematodes from plant material. Journal of Helminthology, 28, 115–118.

    Article  PubMed  CAS  Google Scholar 

  • Stirling, G., Wilson, E., Stirling, A., Pankhurst, C., Moody, P., Bell, M., & Halpin, N. (2005). Amendments of sugarcane trash induce suppressiveness to plant-parasitic nematodes in a sugarcane soil. Australasian Plant Pathology, 34, 203–211.

    Article  Google Scholar 

  • Stock, S. P., & Nadler, S. A. (2006). Morphological and molecular characterisation of Panagrellus spp. (Cephalobina: Panagrolaimidae): Taxonomic status and phylogenetic relationships. Nematology, 8, 921–938.

    Article  CAS  Google Scholar 

  • Tunlid, A., Johansson, T., & Nordbring-Hertz, B. (1991). Surface polymers of the nematode-trapping fungus Arthrobotrys oligospora. Journal of General Microbiology, 137, 1231–1240.

    Article  PubMed  CAS  Google Scholar 

  • Vaitkevicius, K., Lindmark, B., Ou, G., Song, T., Toma, C., Iwanaga, M., Zhu, J., Andersson, A., Hammarström, M. L., Wai, S. N., Tuck, S. (2006). A Vibrio cholerae protease needed for killing of Caenorhabditis elegans has a role in protection from natural predator grazing. Proceedings of the National Academy of Sciences, 103, 9280–9285.

    Google Scholar 

  • Volkmann-Kohlmeyer, B., & Kohlmeyer, J. (1996). How to prepare truly permanent microscope slides. Mycologist, 10, 107–108.

    Article  Google Scholar 

  • Von Sengbusch, P., Hechler, J., & Müller, U. (1983). Molecular architecture of fungal cell walls. An approach by use of fluorescent markers. European Journal of Cell Biology, 30, 305–312.

    PubMed  CAS  Google Scholar 

  • Wang, J., & Higgins, V. J. (2005). Nitric oxide has a regulatory effect in the germination of conidia of Colletotrichum coccodes. Fungal Genetics and Biology, 42, 284–292.

    Article  PubMed  CAS  Google Scholar 

  • White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: a guide to methods and applications. (Innis, M. A., Gelfand, D. H., Sninsky, J. J., White, T. J., eds). Academic Press, New York, USA: 315–322.

    Google Scholar 

  • Whitehead, A., & Hemming, J. (1965). A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology, 55, 25–38.

    Article  Google Scholar 

  • Wilchek, M., & Bayer, E. (1990). Methods in enzymology. San Diego: Academic Press Inc.

    Google Scholar 

  • Wood, F. (1973). Nematode-trapping fungi from a tussock grassland soil in New Zealand. New Zealand Journal of Botany, 11, 231–240.

    Article  Google Scholar 

  • Wyborn, C., Priest, D., & Duddington, C. (1969). Selective technique for the determination of nematophagous fungi in soils. Soil Biology and Biochemistry, 1, 101–102.

    Article  Google Scholar 

  • Xu, L. L., Lai, Y. L., Wang, L., & Liu, X. Z. (2011). Effects of abscisic acid and nitric oxide on trap formation and trapping of nematodes by the fungus Drechslerella stenobrocha AS6.1 Fungal Biology, 2 (114), 97–101.

    Google Scholar 

  • Yang, Y., Yang, E., An, Z., & Liu, X. (2007) Evolution of nematode-trapping cells of predatory fungi of the Orbiliaceae based on evidence from rRNA-encoding DNA and multiprotein sequences. Proceedings of the National Academy of Sciences, USA, 104, 83–79.

    Google Scholar 

  • Zhang, J., Mo, M., Deng, J., Liu, X. F., Bi, T. J., & Zhang, K. Q. (2005). Dactylella zhongdianensis sp. nov, a new predacious antagonist of nematodes. Mycotaxon, 92, 289–294.

    Google Scholar 

  • Zuckerman, B., Matheny, M., & Acosta, N. (1994). Control of plant-parasitic nematodes by a nematicidal strain of Aspergillus niger. Journal of Chemical Ecology, 20, 33–43.

    Article  PubMed  CAS  Google Scholar 

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Li, J., Hyde, K., Zhang, KQ. (2014). Methodology for Studying Nematophagous Fungi. In: Zhang, KQ., Hyde, K. (eds) Nematode-Trapping Fungi. Fungal Diversity Research Series, vol 23. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-8730-7_2

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