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## SERVER: tiger
## EXAMPLE: /BiO/BioProjects/TBD180101-SCHU-Fungi-smallRNA-20180322

## ANALYSIS WORKFLOW

1. Copy to Working Dir

├── NSSP.conf
├── NSSP.py
├── NSSP_util
│   ├── CalculationOfFPKM.py
│   ├── SAMTOOLSConsensusSequence.v.1.0.0.py
│   ├── SAMTOOLSConsensusSequence.v.1.0.1.py
│   ├── SAMTOOLSConsensusSequence_NoGap.py
│   ├── SequenceInfo.fasta.py
│   ├── addReadCnt.py
│   ├── bamStatsInfo.py
│   ├── clc_sequence_info
│   ├── do_mergeExpAnno.py
│   ├── extGenName4sRNA.py
│   ├── extGenName4sRNA_multi.py
│   ├── modMergedConsensus.py
│   └── smRNA_filter.py
└── README

2. Ready for NSSP Run

$source activate smrna
$ln -s [/BiO/BioResources/References/Fusarium_graminearumph1v32/F_graminearumph1v32_biomax_1/F_graminearumph1v32_biomax_1.chr.fa] ref.fa
$ln -s [p3_p13839_Fus_grami_v32.gtf] ref.gtf
$bowtie2-build ref.fa ref.fa

3. RawData CleanUp (paired end -> 50bp single end)

4. modified conf file
######################################
#
#  NSSP
#  Non-human Species Smallrna-seq Pipeline
#  small RNA seq PIPELINE (hardcoding)
#  For Non-model species
#
# [SAMPLE_ID] [PATH]
#
######################################
OE348GFP-2 /BiO/BioProjects/TBD180101-SCHU-Fungi-smallRNA-20180322/RawData/Trimming/trimmedFqFiles/OE348GFP-2_1.fq.gz
R7491_3 /BiO/BioProjects/TBD180101-SCHU-Fungi-smallRNA-20180322/RawData/Trimming/trimmedFqFiles/R7491_3_1.fq.gz

5. RUN

$python NSSP.py NSSP.conf

## RESULT
├── do_cutadapt
│   ├── OE348GFP-2
│   │   ├── OE348GFP-2.clean.fastq (FINAL)
│   │   ├── OE348GFP-2.clean.fastq.log
│   │   ├── OE348GFP-2.clean.fastq.SeqInfo
│   │   ├── OE348GFP-2.clean.pre.fastq
│   │   ├── OE348GFP-2.clean.pre.fastq.log
│   │   ├── OE348GFP-2.fragments.fastq
│   │   └── OE348GFP-2.short.fastq
│   └── R7491_3
│       ├── R7491_3.clean.fastq (FINAL)
│       ├── R7491_3.clean.fastq.log
│       ├── R7491_3.clean.fastq.SeqInfo
│       ├── R7491_3.clean.pre.fastq
│       ├── R7491_3.clean.pre.fastq.log
│       ├── R7491_3.fragments.fastq
│       └── R7491_3.short.fastq
├── do_bowtie2
│   ├── OE348GFP-2
│   │   ├── OE348GFP-2.bowtie2.bam
│   │   ├── OE348GFP-2.bowtie2.sam
│   │   ├── OE348GFP-2.bowtie2.sam.log
│   │   ├── OE348GFP-2.bowtie2.sorted.bam
│   │   └── OE348GFP-2.bowtie2.sorted.bam.stats
│   └── R7491_3
│       ├── R7491_3.bowtie2.bam
│       ├── R7491_3.bowtie2.sam
│       ├── R7491_3.bowtie2.sam.log
│       ├── R7491_3.bowtie2.sorted.bam
│       └── R7491_3.bowtie2.sorted.bam.stats
├── do_extConSeq
│   ├── merged.bam
│   ├── merged.consensus.fa
│   ├── merged.consensus_mod.fa
│   ├── merged.consensus_mod.fa.1.bt2
│   ├── merged.consensus_mod.fa.2.bt2
│   ├── merged.consensus_mod.fa.3.bt2
│   ├── merged.consensus_mod.fa.4.bt2
│   ├── merged.consensus_mod.fa.bt2.log
│   ├── merged.consensus_mod.fa.rev.1.bt2
│   ├── merged.consensus_mod.fa.rev.2.bt2
│   ├── merged.consensus_mod.fa.SeqInfo
│   └── merged.sorted.bam
├── do_expConSeq
│   ├── OE348GFP-2
│   │   ├── OE348GFP-2.bowtie2.bam
│   │   ├── OE348GFP-2.bowtie2.sam
│   │   ├── OE348GFP-2.bowtie2.sam.log
│   │   ├── OE348GFP-2.bowtie2.sorted.bam
│   │   ├── OE348GFP-2.bowtie2.sorted.bam.bai
│   │   ├── OE348GFP-2.bowtie2.sorted.bam.FPKM.xls
│   │   ├── OE348GFP-2.bowtie2.sorted.bam.idxstats
│   │   ├── OE348GFP-2.bowtie2.sorted.bam.ReadCnt.xls
│   │   └── OE348GFP-2.bowtie2.sorted.bam.stats
│   └── R7491_3
│       ├── R7491_3.bowtie2.bam
│       ├── R7491_3.bowtie2.sam
│       ├── R7491_3.bowtie2.sam.log
│       ├── R7491_3.bowtie2.sorted.bam
│       ├── R7491_3.bowtie2.sorted.bam.bai
│       ├── R7491_3.bowtie2.sorted.bam.FPKM.xls
│       ├── R7491_3.bowtie2.sorted.bam.idxstats
│       ├── R7491_3.bowtie2.sorted.bam.ReadCnt.xls
│       └── R7491_3.bowtie2.sorted.bam.stats
├── do_multiExp
│   └── multi.Exp
├── do_annoGene
│   ├── extGenName4sRNA_result.supercontig_3.1
│   ├── extGenName4sRNA_result.supercontig_3.10
│   ├── extGenName4sRNA_result.supercontig_3.11
│   ├── extGenName4sRNA_result.supercontig_3.12
│   ├── extGenName4sRNA_result.supercontig_3.13
│   ├── extGenName4sRNA_result.supercontig_3.14
│   ├── extGenName4sRNA_result.supercontig_3.15
│   ├── extGenName4sRNA_result.supercontig_3.16
│   ├── extGenName4sRNA_result.supercontig_3.17
│   ├── extGenName4sRNA_result.supercontig_3.18
│   ├── extGenName4sRNA_result.supercontig_3.19
│   ├── extGenName4sRNA_result.supercontig_3.2
│   ├── extGenName4sRNA_result.supercontig_3.20
│   ├── extGenName4sRNA_result.supercontig_3.21
│   ├── extGenName4sRNA_result.supercontig_3.22
│   ├── extGenName4sRNA_result.supercontig_3.24
│   ├── extGenName4sRNA_result.supercontig_3.25
│   ├── extGenName4sRNA_result.supercontig_3.26
│   ├── extGenName4sRNA_result.supercontig_3.27
│   ├── extGenName4sRNA_result.supercontig_3.28
│   ├── extGenName4sRNA_result.supercontig_3.29
│   ├── extGenName4sRNA_result.supercontig_3.3
│   ├── extGenName4sRNA_result.supercontig_3.31
│   ├── extGenName4sRNA_result.supercontig_3.4
│   ├── extGenName4sRNA_result.supercontig_3.5
│   ├── extGenName4sRNA_result.supercontig_3.6
│   ├── extGenName4sRNA_result.supercontig_3.7
│   ├── extGenName4sRNA_result.supercontig_3.8
│   ├── extGenName4sRNA_result.supercontig_3.9
│   ├── extGenName4sRNA_result.xls (FINAL)
│   └── temp
├── do_mergeExpAnno
│   ├── smRNAs.d100.xls
│   └── smRNAs.xls
├── do_summary
│   ├── Clean
│   │   ├── Clean.SeqInfo.Report
│   │   ├── OE348GFP-2.clean.fastq -> /BiO/BioProjects/TBD180101-SCHU-Fungi-smallRNA-20180322/do_cutadapt/OE348GFP-2/OE348GFP-2.clean.fastq
│   │   └── R7491_3.clean.fastq -> /BiO/BioProjects/TBD180101-SCHU-Fungi-smallRNA-20180322/do_cutadapt/R7491_3/R7491_3.clean.fastq
│   ├── ExtConSeq
│   │   ├── extconseq.SeqInfo.Report
│   │   └── merged.consensus_mod.fa -> /BiO/BioProjects/TBD180101-SCHU-Fungi-smallRNA-20180322/do_extConSeq/merged.consensus_mod.fa
│   ├── Mapping
│   │   ├── Mapping.MapInfo.Report
│   │   ├── OE348GFP-2.bowtie2.bam -> /BiO/BioProjects/TBD180101-SCHU-Fungi-smallRNA-20180322/do_bowtie2/OE348GFP-2/OE348GFP-2.bowtie2.bam
│   │   └── R7491_3.bowtie2.bam -> /BiO/BioProjects/TBD180101-SCHU-Fungi-smallRNA-20180322/do_bowtie2/R7491_3/R7491_3.bowtie2.bam
│   ├── Raw
│   │   ├── OE348GFP-2_1.fq.gz -> /BiO/BioProjects/TBD180101-SCHU-Fungi-smallRNA-20180322/RawData/Trimming/trimmedFqFiles/OE348GFP-2_1.fq.gz
│   │   ├── R7491_3_1.fq.gz -> /BiO/BioProjects/TBD180101-SCHU-Fungi-smallRNA-20180322/RawData/Trimming/trimmedFqFiles/R7491_3_1.fq.gz
│   │   └── Raw.SeqInfo.Report
│   └── smRNAs.xls -> /BiO/BioProjects/TBD180101-SCHU-Fungi-smallRNA-20180322/do_mergeExpAnno/smRNAs.xls

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