Kevin Blighe, Aaron Lun 2021-07-23
Principal Component Analysis (PCA) is a very powerful technique that has wide applicability in data science, bioinformatics, and further afield. It was initially developed to analyse large volumes of data in order to tease out the differences/relationships between the logical entities being analysed. It extracts the fundamental structure of the data without the need to build any model to represent it. This ‘summary’ of the data is arrived at through a process of reduction that can transform the large number of variables into a lesser number that are uncorrelated (i.e. the ‘principal components’), while at the same time being capable of easy interpretation on the original data (Blighe and Lun 2019) (Blighe 2013).
PCAtools provides functions for data exploration via PCA, and allows the user to generate publication-ready figures. PCA is performed via BiocSingular (Lun 2019) - users can also identify optimal number of principal components via different metrics, such as elbow method and Horn’s parallel analysis (Horn 1965) (Buja and Eyuboglu 1992), which has relevance for data reduction in single-cell RNA-seq (scRNA-seq) and high dimensional mass cytometry data.
if (!requireNamespace('BiocManager', quietly = TRUE))
install.packages('BiocManager')
BiocManager::install('PCAtools')Note: to install development version direct from GitHub:
if (!requireNamespace('devtools', quietly = TRUE))
install.packages('devtools')
devtools::install_github('kevinblighe/PCAtools') library(PCAtools)For this example, we will follow the tutorial (from Section 3.1) of RNA-seq workflow: gene-level exploratory analysis and differential expression. Specifically, we will load the ‘airway’ data, where different airway smooth muscle cells were treated with dexamethasone.
library(airway)
library(magrittr)
data('airway')
airway$dex %<>% relevel('untrt')Annotate the Ensembl gene IDs to gene symbols:
ens <- rownames(airway)
library(org.Hs.eg.db)
symbols <- mapIds(org.Hs.eg.db, keys = ens,
column = c('SYMBOL'), keytype = 'ENSEMBL')
symbols <- symbols[!is.na(symbols)]
symbols <- symbols[match(rownames(airway), names(symbols))]
rownames(airway) <- symbols
keep <- !is.na(rownames(airway))
airway <- airway[keep,]Normalise the data and transform the normalised counts to variance-stabilised expression levels:
library('DESeq2')
dds <- DESeqDataSet(airway, design = ~ cell + dex)
dds <- DESeq(dds)
vst <- assay(vst(dds)) p <- pca(vst, metadata = colData(airway), removeVar = 0.1)## -- removing the lower 10% of variables based on variance
screeplot(p, axisLabSize = 18, titleLabSize = 22)Different interpretations of the biplot exist. In the OMICs era, for most general users, a biplot is a simple representation of samples in a 2-dimensional space, usually focusing on just the first two PCs:
biplot(p)However, the original definition of a biplot by Gabriel KR (Gabriel 1971) is a plot that plots both variables and observations (samples) in the same space. The variables are indicated by arrows drawn from the origin, which indicate their ‘weight’ in different directions. We touch on this later via the plotLoadings function.
biplot(p, showLoadings = TRUE,
labSize = 5, pointSize = 5, sizeLoadingsNames = 5)Here, we will instead start with data from Gene Expression Omnibus. We will load breast cancer gene expression data with recurrence free survival (RFS) from Gene Expression Profiling in Breast Cancer: Understanding the Molecular Basis of Histologic Grade To Improve Prognosis.
First, let’s read in and prepare the data:
library(Biobase)
library(GEOquery)
# load series and platform data from GEO
gset <- getGEO('GSE2990', GSEMatrix = TRUE, getGPL = FALSE)
mat <- exprs(gset[[1]])
# remove Affymetrix control probes
mat <- mat[-grep('^AFFX', rownames(mat)),]
# extract information of interest from the phenotype data (pdata)
idx <- which(colnames(pData(gset[[1]])) %in%
c('relation', 'age:ch1', 'distant rfs:ch1', 'er:ch1',
'ggi:ch1', 'grade:ch1', 'size:ch1',
'time rfs:ch1'))
metadata <- data.frame(pData(gset[[1]])[,idx],
row.names = rownames(pData(gset[[1]])))
# tidy column names
colnames(metadata) <- c('Study', 'Age', 'Distant.RFS', 'ER', 'GGI', 'Grade',
'Size', 'Time.RFS')
# prepare certain phenotypes of interest
metadata$Study <- gsub('Reanalyzed by: ', '', as.character(metadata$Study))
metadata$Age <- as.numeric(gsub('^KJ', NA, as.character(metadata$Age)))
metadata$Distant.RFS <- factor(metadata$Distant.RFS,
levels = c(0,1))
metadata$ER <- factor(gsub('\\?', NA, as.character(metadata$ER)),
levels = c(0,1))
metadata$ER <- factor(ifelse(metadata$ER == 1, 'ER+', 'ER-'),
levels = c('ER-', 'ER+'))
metadata$GGI <- as.numeric(as.character(metadata$GGI))
metadata$Grade <- factor(gsub('\\?', NA, as.character(metadata$Grade)),
levels = c(1,2,3))
metadata$Grade <- gsub(1, 'Grade 1', gsub(2, 'Grade 2', gsub(3, 'Grade 3', metadata$Grade)))
metadata$Grade <- factor(metadata$Grade, levels = c('Grade 1', 'Grade 2', 'Grade 3'))
metadata$Size <- as.numeric(as.character(metadata$Size))
metadata$Time.RFS <- as.numeric(gsub('^KJX|^KJ', NA, metadata$Time.RFS))
# remove samples from the pdata that have any NA value
discard <- apply(metadata, 1, function(x) any(is.na(x)))
metadata <- metadata[!discard,]
# filter the expression data to match the samples in our pdata
mat <- mat[,which(colnames(mat) %in% rownames(metadata))]
# check that sample names match exactly between pdata and expression data
all(colnames(mat) == rownames(metadata))## [1] TRUE
Conduct principal component analysis (PCA):
p <- pca(mat, metadata = metadata, removeVar = 0.1)
## -- removing the lower 10% of variables based on variance
biplot(p) biplot(p, showLoadings = TRUE, lab = NULL)One of the probes pointing downward is 205225_at, which targets the ESR1 gene. This is already a useful validation, as the oestrogen receptor, which is in part encoded by ESR1, is strongly represented by PC2 (y-axis), with negative-to-positive receptor status going from top-to-bottom.
More on this later in this vignette.
pairsplot(p)If the biplot was previously generated with showLoadings = TRUE, check how this loadings plot corresponds to the biplot loadings - they should match up for the top hits.
plotloadings(p, labSize = 3)## -- variables retained:
## 215281_x_at, 214464_at, 211122_s_at, 210163_at, 204533_at, 205225_at, 209351_at, 205044_at, 202037_s_at, 204540_at, 215176_x_at, 214768_x_at, 212671_s_at, 219415_at, 37892_at, 208650_s_at, 206754_s_at, 205358_at, 205380_at, 205825_at
## Warning: ggrepel: 17 unlabeled data points (too many overlaps). Consider
## increasing max.overlaps
eigencorplot(p,
metavars = c('Study','Age','Distant.RFS','ER',
'GGI','Grade','Size','Time.RFS'))The rotated data that represents the observations / samples is stored in rotated, while the variable loadings are stored in loadings
p$rotated[1:5,1:5]## PC1 PC2 PC3 PC4 PC5
## GSM65752 -30.24272 43.826310 3.781677 -39.536149 18.612835
## GSM65753 -37.73436 -15.464421 -4.913100 -5.877623 9.060108
## GSM65755 -29.95155 7.788280 -22.980076 -15.222649 23.123766
## GSM65757 -33.73509 1.261410 -22.834375 2.494554 13.629207
## GSM65758 -40.95958 -8.588458 4.995440 14.340150 0.417101
p$loadings[1:5,1:5]## PC1 PC2 PC3 PC4 PC5
## 206378_at -0.0024336244 -0.05312797 -0.004809456 0.04045087 0.0096616577
## 205916_at -0.0051057533 0.00122765 -0.010593760 0.04023264 0.0285972617
## 206799_at 0.0005723191 -0.05048096 -0.009992964 0.02568142 0.0024626261
## 205242_at 0.0129147329 0.02867789 0.007220832 0.04424070 -0.0006138609
## 206509_at 0.0019058729 -0.05447596 -0.004979062 0.01510060 -0.0026213610
All functions in PCAtools are highly configurable and should cover virtually all basic and advanced user requirements. The following sections take a look at some of these advanced features, and form a somewhat practical example of how one can use PCAtools to make a clinical interpretation of data.
First, let’s sort out the gene annotation by mapping the probe IDs to gene symbols. The array used for this study was the Affymetrix U133a, so let’s use the hgu133a.db Bioconductor package:
suppressMessages(require(hgu133a.db))
newnames <- mapIds(hgu133a.db,
keys = rownames(p$loadings),
column = c('SYMBOL'),
keytype = 'PROBEID')## 'select()' returned 1:many mapping between keys and columns
# tidy up for NULL mappings and duplicated gene symbols
newnames <- ifelse(is.na(newnames) | duplicated(newnames),
names(newnames), newnames)
rownames(p$loadings) <- newnamesA scree plot on its own just shows the accumulative proportion of explained variation, but how can we determine the optimum number of PCs to retain?
PCAtools provides four metrics for this purpose:
- Elbow method
- Horn’s parallel analysis (Horn 1965) (Buja and Eyuboglu 1992).
- Marchenko-Pastur limit
- Gavish-Donoho method
Let’s perform Horn’s parallel analysis first:
horn <- parallelPCA(mat)
horn$n## [1] 11
Now the elbow method:
elbow <- findElbowPoint(p$variance)
elbow## PC8
## 8
In most cases, the identified values will disagree. This is because finding the correct number of PCs is a difficult task and is akin to finding the ‘correct’ number of clusters in a dataset - there is no correct answer.
Taking these values, we can produce a new scree plot and mark these:
library(ggplot2)
screeplot(p,
components = getComponents(p, 1:20),
vline = c(horn$n, elbow)) +
geom_label(aes(x = horn$n + 1, y = 50,
label = 'Horn\'s', vjust = -1, size = 8)) +
geom_label(aes(x = elbow + 1, y = 50,
label = 'Elbow method', vjust = -1, size = 8))If all else fails, one can simply take the number of PCs that contributes to a pre-selected total of explained variation, e.g., in this case, 27 PCs account for >80% explained variation.
which(cumsum(p$variance) > 80)[1]## PC27
## 27
The bi-plot comparing PC1 versus PC2 is the most characteristic plot of PCA. However, PCA is much more than the bi-plot and much more than PC1 and PC2. This said, PC1 and PC2, by the very nature of PCA, are indeed usually the most important parts of a PCA analysis.
In a bi-plot, we can shade the points by different groups and add many more features.
biplot(p,
lab = paste0(p$metadata$Age, ' años'),
colby = 'ER',
hline = 0, vline = 0,
legendPosition = 'right')## Warning: ggrepel: 20 unlabeled data points (too many overlaps). Consider
## increasing max.overlaps
The encircle functionality literally draws a polygon around each group specified by colby. It says nothing about any statistic pertaining to each group.
biplot(p,
colby = 'ER', colkey = c('ER+' = 'forestgreen', 'ER-' = 'purple'),
colLegendTitle = 'ER-\nstatus',
# encircle config
encircle = TRUE,
encircleFill = TRUE,
hline = 0, vline = c(-25, 0, 25),
legendPosition = 'top', legendLabSize = 16, legendIconSize = 8.0)## Warning: ggrepel: 26 unlabeled data points (too many overlaps). Consider
## increasing max.overlaps
biplot(p,
colby = 'ER', colkey = c('ER+' = 'forestgreen', 'ER-' = 'purple'),
colLegendTitle = 'ER-\nstatus',
# encircle config
encircle = TRUE, encircleFill = FALSE,
encircleAlpha = 1, encircleLineSize = 5,
hline = 0, vline = c(-25, 0, 25),
legendPosition = 'top', legendLabSize = 16, legendIconSize = 8.0)## Warning: ggrepel: 26 unlabeled data points (too many overlaps). Consider
## increasing max.overlaps
Stat ellipses are also drawn around each group but have a greater statistical meaning and can be used, for example, as a strict determination of outlier samples. Here, we draw ellipses around each group at the 95% confidence level:
biplot(p,
colby = 'ER', colkey = c('ER+' = 'forestgreen', 'ER-' = 'purple'),
# ellipse config
ellipse = TRUE,
ellipseType = 't',
ellipseLevel = 0.95,
ellipseFill = TRUE,
ellipseAlpha = 1/4,
ellipseLineSize = 1.0,
xlim = c(-125,125), ylim = c(-50, 80),
hline = 0, vline = c(-25, 0, 25),
legendPosition = 'top', legendLabSize = 16, legendIconSize = 8.0)## Warning: ggrepel: 40 unlabeled data points (too many overlaps). Consider
## increasing max.overlaps
biplot(p,
colby = 'ER', colkey = c('ER+' = 'forestgreen', 'ER-' = 'purple'),
# ellipse config
ellipse = TRUE,
ellipseType = 't',
ellipseLevel = 0.95,
ellipseFill = TRUE,
ellipseAlpha = 1/4,
ellipseLineSize = 0,
ellipseFillKey = c('ER+' = 'yellow', 'ER-' = 'pink'),
xlim = c(-125,125), ylim = c(-50, 80),
hline = 0, vline = c(-25, 0, 25),
legendPosition = 'top', legendLabSize = 16, legendIconSize = 8.0)## Warning: ggrepel: 40 unlabeled data points (too many overlaps). Consider
## increasing max.overlaps
biplot(p,
colby = 'ER', colkey = c('ER+' = 'forestgreen', 'ER-' = 'purple'),
hline = c(-25, 0, 25), vline = c(-25, 0, 25),
legendPosition = 'top', legendLabSize = 13, legendIconSize = 8.0,
shape = 'Grade', shapekey = c('Grade 1' = 15, 'Grade 2' = 17, 'Grade 3' = 8),
drawConnectors = FALSE,
title = 'PCA bi-plot',
subtitle = 'PC1 versus PC2',
caption = '27 PCs ≈ 80%') biplot(p,
lab = NULL,
colby = 'ER', colkey = c('ER+'='royalblue', 'ER-'='red3'),
hline = c(-25, 0, 25), vline = c(-25, 0, 25),
vlineType = c('dotdash', 'solid', 'dashed'),
gridlines.major = FALSE, gridlines.minor = FALSE,
pointSize = 5,
legendPosition = 'left', legendLabSize = 14, legendIconSize = 8.0,
shape = 'Grade', shapekey = c('Grade 1'=15, 'Grade 2'=17, 'Grade 3'=8),
drawConnectors = FALSE,
title = 'PCA bi-plot',
subtitle = 'PC1 versus PC2',
caption = '27 PCs ≈ 80%')Let’s plot the same as above but with loadings:
biplot(p,
# loadings parameters
showLoadings = TRUE,
lengthLoadingsArrowsFactor = 1.5,
sizeLoadingsNames = 4,
colLoadingsNames = 'red4',
# other parameters
lab = NULL,
colby = 'ER', colkey = c('ER+'='royalblue', 'ER-'='red3'),
hline = 0, vline = c(-25, 0, 25),
vlineType = c('dotdash', 'solid', 'dashed'),
gridlines.major = FALSE, gridlines.minor = FALSE,
pointSize = 5,
legendPosition = 'left', legendLabSize = 14, legendIconSize = 8.0,
shape = 'Grade', shapekey = c('Grade 1'=15, 'Grade 2'=17, 'Grade 3'=8),
drawConnectors = FALSE,
title = 'PCA bi-plot',
subtitle = 'PC1 versus PC2',
caption = '27 PCs ≈ 80%')There are two ways to colour by a continuous variable. In the first way, we simply ‘add on’ a continuous colour scale via scale_colour_gradient:
# add ESR1 gene expression to the metadata
p$metadata$ESR1 <- mat['205225_at',]
biplot(p,
x = 'PC2', y = 'PC3',
lab = NULL,
colby = 'ESR1',
shape = 'ER',
hline = 0, vline = 0,
legendPosition = 'right') +
scale_colour_gradient(low = 'gold', high = 'red2')We can also just permit that the internal ggplot2 engine picks the colour scheme - here, we also plot PC10 versus PC50:
biplot(p, x = 'PC10', y = 'PC50',
lab = NULL,
colby = 'Age',
hline = 0, vline = 0,
hlineWidth = 1.0, vlineWidth = 1.0,
gridlines.major = FALSE, gridlines.minor = TRUE,
pointSize = 5,
legendPosition = 'left', legendLabSize = 16, legendIconSize = 8.0,
shape = 'Grade', shapekey = c('Grade 1'=15, 'Grade 2'=17, 'Grade 3'=8),
drawConnectors = FALSE,
title = 'PCA bi-plot',
subtitle = 'PC10 versus PC50',
caption = '27 PCs ≈ 80%')The pairs plot in PCA unfortunately suffers from a lack of use; however, for those who love exploring data and squeezing every last ounce of information out of data, a pairs plot provides for a relatively quick way to explore useful leads for other downstream analyses.
As the number of pairwise plots increases, however, space becomes limited. We can shut off titles and axis labeling to save space. Reducing point size and colouring by a variable of interest can additionally help us to rapidly skim over the data.
pairsplot(p,
components = getComponents(p, c(1:10)),
triangle = TRUE, trianglelabSize = 12,
hline = 0, vline = 0,
pointSize = 0.4,
gridlines.major = FALSE, gridlines.minor = FALSE,
colby = 'Grade',
title = 'Pairs plot', plotaxes = FALSE,
margingaps = unit(c(-0.01, -0.01, -0.01, -0.01), 'cm'))We can arrange these in a way that makes better use of the screen space by setting ‘triangle = FALSE’. In this case, we can further control the layout with the ‘ncol’ and ‘nrow’ parameters, although, the function will automatically determine these based on your input data.
pairsplot(p,
components = getComponents(p, c(4,33,11,1)),
triangle = FALSE,
hline = 0, vline = 0,
pointSize = 0.8,
gridlines.major = FALSE, gridlines.minor = FALSE,
colby = 'ER',
title = 'Pairs plot', titleLabSize = 22,
axisLabSize = 14, plotaxes = TRUE,
margingaps = unit(c(0.1, 0.1, 0.1, 0.1), 'cm'))If, on the bi-plot or pairs plot, we encounter evidence that 1 or more PCs are segregating a factor of interest, we can explore further the genes that are driving these differences along each PC.
For each PC of interest, ‘plotloadings’ determines the variables falling within the top/bottom 5% of the loadings range, and then creates a final consensus list of these. These variables are then plotted.
The loadings plot, like all others, is highly configurable. To modify the cut-off for inclusion / exclusion of variables, we use rangeRetain, where 0.01 equates to the top/bottom 1% of the loadings range per PC.
plotloadings(p,
rangeRetain = 0.01,
labSize = 4.0,
title = 'Loadings plot',
subtitle = 'PC1, PC2, PC3, PC4, PC5',
caption = 'Top 1% variables',
shape = 24,
col = c('limegreen', 'black', 'red3'),
drawConnectors = TRUE)## -- variables retained:
## POGZ, CDC42BPA, CXCL11, ESR1, SFRP1, EEF1A2, IGKC, GABRP, CD24, PDZK1
## Warning: ggrepel: 1 unlabeled data points (too many overlaps). Consider
## increasing max.overlaps
At least one interesting finding is 205225_at / ESR1, which is by far the gene most responsible for variation along PC2. The previous bi-plots showed that this PC also segregated ER+ from ER- patients. The other results could be explored. Also, from the biplots with loadings that we have already generated, this result is also verified in these.
With the loadings plot, in addition, we can instead plot absolute values and modify the point sizes to be proportional to the loadings. We can also switch off the line connectors and plot the loadings for any PCs
plotloadings(p,
components = getComponents(p, c(4,33,11,1)),
rangeRetain = 0.1,
labSize = 4.0,
absolute = FALSE,
title = 'Loadings plot',
subtitle = 'Misc PCs',
caption = 'Top 10% variables',
shape = 23, shapeSizeRange = c(1, 16),
col = c('white', 'pink'),
drawConnectors = FALSE)## -- variables retained:
## CXCL11, IGKC, CXCL9, 210163_at, 214768_x_at, 211645_x_at, 211644_x_at, IGHA1, 216491_x_at, 214777_at, 216576_x_at, 212671_s_at, IL23A, PLAAT4, 212588_at, 212998_x_at, KRT14, GABRP, SOX10, PTX3, TTYH1, CPB1, KRT15, MYBPC1, DST, CXADR, GALNT3, CDH3, TCIM, DHRS2, MMP1, CRABP1, CST1, MAGEA3, ACOX2, PRKAR2B, PLCB1, HDGFL3, CYP2B6, ORM1, 205040_at, HSPB8, SCGB2A2, JCHAIN, POGZ, 213872_at, DYNC2LI1, CDC42BPA
We can plot just this single PC and flip the plot on its side, if we wish:
plotloadings(p,
components = getComponents(p, c(2)),
rangeRetain = 0.12, absolute = TRUE,
col = c('black', 'pink', 'red4'),
drawConnectors = TRUE, labSize = 4) + coord_flip()## -- variables retained:
## S100A8, PROM1, CXCL11, MMP1, FABP7, 205029_s_at, CXCL9, 210163_at, UBD, IGHG3, RARRES1, 206392_s_at, CXCL10, GBP1, ASPM, CDC20, NAT1, ESR1, SCUBE2
## Warning: ggrepel: 2 unlabeled data points (too many overlaps). Consider
## increasing max.overlaps
Further exploration of the PCs can come through correlations with clinical data. This is also a mostly untapped resource in the era of ‘big data’ and can help to guide an analysis down a particular path.
We may wish, for example, to correlate all PCs that account for 80% variation in our dataset and then explore further the PCs that have statistically significant correlations.
‘eigencorplot’ is built upon another function by the PCAtools developers, namely CorLevelPlot. Further examples can be found there.
eigencorplot(p,
components = getComponents(p, 1:27),
metavars = c('Study','Age','Distant.RFS','ER',
'GGI','Grade','Size','Time.RFS'),
col = c('darkblue', 'blue2', 'black', 'red2', 'darkred'),
cexCorval = 0.7,
colCorval = 'white',
fontCorval = 2,
posLab = 'bottomleft',
rotLabX = 45,
posColKey = 'top',
cexLabColKey = 1.5,
scale = TRUE,
main = 'PC1-27 clinical correlations',
colFrame = 'white',
plotRsquared = FALSE)We can also supply different cut-offs for statistical significance, apply p-value adjustment, plot R-squared values, and specify correlation method:
eigencorplot(p,
components = getComponents(p, 1:horn$n),
metavars = c('Study','Age','Distant.RFS','ER','GGI',
'Grade','Size','Time.RFS'),
col = c('white', 'cornsilk1', 'gold', 'forestgreen', 'darkgreen'),
cexCorval = 1.2,
fontCorval = 2,
posLab = 'all',
rotLabX = 45,
scale = TRUE,
main = bquote(Principal ~ component ~ Pearson ~ r^2 ~ clinical ~ correlates),
plotRsquared = TRUE,
corFUN = 'pearson',
corUSE = 'pairwise.complete.obs',
corMultipleTestCorrection = 'BH',
signifSymbols = c('****', '***', '**', '*', ''),
signifCutpoints = c(0, 0.0001, 0.001, 0.01, 0.05, 1))Clearly, PC2 is coming across as the most interesting PC in this experiment, with highly statistically significant correlation (p<0.0001) to ER status, tumour grade, and GGI (genomic Grade Index), an indicator of response. It comes as no surprise that the gene driving most variationn along PC2 is ESR1, identified from our loadings plot.
This information is, of course, not new, but shows how PCA is much more than just a bi-plot used to identify outliers!
pscree <- screeplot(p, components = getComponents(p, 1:30),
hline = 80, vline = 27, axisLabSize = 14, titleLabSize = 20,
returnPlot = FALSE) +
geom_label(aes(20, 80, label = '80% explained variation', vjust = -1, size = 8))
ppairs <- pairsplot(p, components = getComponents(p, c(1:3)),
triangle = TRUE, trianglelabSize = 12,
hline = 0, vline = 0,
pointSize = 0.8, gridlines.major = FALSE, gridlines.minor = FALSE,
colby = 'Grade',
title = '', plotaxes = FALSE,
margingaps = unit(c(0.01, 0.01, 0.01, 0.01), 'cm'),
returnPlot = FALSE)
pbiplot <- biplot(p,
# loadings parameters
showLoadings = TRUE,
lengthLoadingsArrowsFactor = 1.5,
sizeLoadingsNames = 4,
colLoadingsNames = 'red4',
# other parameters
lab = NULL,
colby = 'ER', colkey = c('ER+'='royalblue', 'ER-'='red3'),
hline = 0, vline = c(-25, 0, 25),
vlineType = c('dotdash', 'solid', 'dashed'),
gridlines.major = FALSE, gridlines.minor = FALSE,
pointSize = 5,
legendPosition = 'none', legendLabSize = 16, legendIconSize = 8.0,
shape = 'Grade', shapekey = c('Grade 1'=15, 'Grade 2'=17, 'Grade 3'=8),
drawConnectors = FALSE,
title = 'PCA bi-plot',
subtitle = 'PC1 versus PC2',
caption = '27 PCs ≈ 80%',
returnPlot = FALSE)
ploadings <- plotloadings(p, rangeRetain = 0.01, labSize = 4,
title = 'Loadings plot', axisLabSize = 12,
subtitle = 'PC1, PC2, PC3, PC4, PC5',
caption = 'Top 1% variables',
shape = 24, shapeSizeRange = c(4, 8),
col = c('limegreen', 'black', 'red3'),
legendPosition = 'none',
drawConnectors = FALSE,
returnPlot = FALSE)
peigencor <- eigencorplot(p,
components = getComponents(p, 1:10),
metavars = c('Study','Age','Distant.RFS','ER',
'GGI','Grade','Size','Time.RFS'),
cexCorval = 1.0,
fontCorval = 2,
posLab = 'all',
rotLabX = 45,
scale = TRUE,
main = "PC clinical correlates",
cexMain = 1.5,
plotRsquared = FALSE,
corFUN = 'pearson',
corUSE = 'pairwise.complete.obs',
signifSymbols = c('****', '***', '**', '*', ''),
signifCutpoints = c(0, 0.0001, 0.001, 0.01, 0.05, 1),
returnPlot = FALSE)
library(cowplot)
library(ggplotify)
top_row <- plot_grid(pscree, ppairs, pbiplot,
ncol = 3,
labels = c('A', 'B Pairs plot', 'C'),
label_fontfamily = 'serif',
label_fontface = 'bold',
label_size = 22,
align = 'h',
rel_widths = c(1.10, 0.80, 1.10))
bottom_row <- plot_grid(ploadings,
as.grob(peigencor),
ncol = 2,
labels = c('D', 'E'),
label_fontfamily = 'serif',
label_fontface = 'bold',
label_size = 22,
align = 'h',
rel_widths = c(0.8, 1.2))
plot_grid(top_row, bottom_row, ncol = 1,
rel_heights = c(1.1, 0.9))It is possible to use the variable loadings as part of a matrix calculation to ‘predict’ principal component eigenvectors in new data. This is elaborated in a posting by Pandula Priyadarshana: How to use Principal Component Analysis (PCA) to make Predictions.
The pca class, which is created by PCAtools, is not configured to work with stats::predict; however, trusty prcomp class is configured. We can manually create a prcomp object and then use that in model prediction, as elaborated in the following code chunk:
p <- pca(mat, metadata = metadata, removeVar = 0.1)## -- removing the lower 10% of variables based on variance
p.prcomp <- list(sdev = p$sdev,
rotation = data.matrix(p$loadings),
x = data.matrix(p$rotated),
center = TRUE, scale = TRUE)
class(p.prcomp) <- 'prcomp'
# for this simple example, just use a chunk of
# the original data for the prediction
newdata <- t(mat[,seq(1,20)])
predict(p.prcomp, newdata = newdata)[,1:5]## PC1 PC2 PC3 PC4 PC5
## GSM65752 11.683293 71.0152986 10.677205 -75.97644152 29.7537169
## GSM65753 -10.542633 -31.9953531 -2.753783 -19.59178967 14.9924713
## GSM65755 6.585509 13.4975310 -40.370389 -29.38990525 47.7142845
## GSM65757 1.498398 -0.1294115 -37.336278 0.08078156 22.3448232
## GSM65758 -18.049833 -14.9445805 14.890320 16.57567005 3.4010033
## GSM65760 8.073473 47.5491189 -18.016340 -9.73629569 -51.7330414
## GSM65761 -3.689814 7.7199606 -35.476666 -35.31465087 -40.1455143
## GSM65762 3.949911 -24.9428080 4.710631 2.71721065 43.2182093
## GSM65763 -20.757238 -33.3085383 22.639443 7.41053224 -9.9339918
## GSM65764 -12.287305 -12.7566718 13.813429 33.75583684 17.7938583
## GSM65767 -4.209505 -13.9349129 -17.814569 -14.87200276 -82.4754172
## GSM65768 3.547044 39.6095431 -28.424912 40.26444836 45.6591355
## GSM65769 3.754370 30.0201461 12.415498 45.74502641 37.9905308
## GSM65770 2.538593 -36.6517740 54.887990 5.94021104 -0.9545218
## GSM65771 -7.382089 -8.5963702 27.749060 -21.50981794 -71.4524526
## GSM65772 3.735223 43.2576570 26.995375 21.01817312 -68.8193200
## GSM65773 15.775812 -19.4523339 4.419158 -6.47899302 -25.2479186
## GSM65774 17.589719 -28.5666333 -52.875007 -16.82207768 37.8455365
## GSM65775 -3.375783 -5.2950960 27.071957 49.10111537 55.0410908
## GSM65776 1.562855 -22.0947718 12.797877 7.08296875 -4.9924828
The development of PCAtools has benefited from contributions and suggestions from:
- Krushna Chandra Murmu
- Jinsheng
- Myles Lewis
- Anna-Leigh Brown
- Vincent Carey
- Vince Vu
- Guido Hooiveld
- pwwang
- Pandula Priyadarshana
- Barley Rose Collier Harris
- Bob Policastro
- Alan O’Callaghan
sessionInfo()## R version 4.0.3 (2020-10-10)
## Platform: x86_64-pc-linux-gnu (64-bit)
## Running under: Ubuntu 16.04.7 LTS
##
## Matrix products: default
## BLAS: /usr/lib/atlas-base/atlas/libblas.so.3.0
## LAPACK: /usr/lib/atlas-base/atlas/liblapack.so.3.0
##
## locale:
## [1] LC_CTYPE=pt_BR.UTF-8 LC_NUMERIC=C
## [3] LC_TIME=en_GB.UTF-8 LC_COLLATE=pt_BR.UTF-8
## [5] LC_MONETARY=en_GB.UTF-8 LC_MESSAGES=pt_BR.UTF-8
## [7] LC_PAPER=en_GB.UTF-8 LC_NAME=C
## [9] LC_ADDRESS=C LC_TELEPHONE=C
## [11] LC_MEASUREMENT=en_GB.UTF-8 LC_IDENTIFICATION=C
##
## attached base packages:
## [1] parallel stats4 stats graphics grDevices utils datasets
## [8] methods base
##
## other attached packages:
## [1] ggplotify_0.0.5 cowplot_1.1.1
## [3] hgu133a.db_3.2.3 GEOquery_2.56.0
## [5] DESeq2_1.28.1 org.Hs.eg.db_3.11.4
## [7] AnnotationDbi_1.53.0 magrittr_2.0.1
## [9] airway_1.8.0 SummarizedExperiment_1.18.2
## [11] DelayedArray_0.14.1 matrixStats_0.57.0
## [13] Biobase_2.48.0 GenomicRanges_1.40.0
## [15] GenomeInfoDb_1.24.2 IRanges_2.22.2
## [17] S4Vectors_0.26.1 BiocGenerics_0.34.0
## [19] PCAtools_2.5.5 ggrepel_0.9.1
## [21] ggplot2_3.3.3
##
## loaded via a namespace (and not attached):
## [1] bitops_1.0-6 bit64_4.0.5
## [3] ash_1.0-15 RColorBrewer_1.1-2
## [5] tools_4.0.3 R6_2.5.0
## [7] irlba_2.3.3 KernSmooth_2.23-18
## [9] DBI_1.1.1 colorspace_2.0-0
## [11] withr_2.4.1 tidyselect_1.1.0
## [13] ggalt_0.4.0 extrafontdb_1.0
## [15] bit_4.0.4 curl_4.3
## [17] compiler_4.0.3 cli_2.2.0
## [19] xml2_1.3.2 labeling_0.4.2
## [21] scales_1.1.1 proj4_1.0-10.1
## [23] readr_1.4.0 genefilter_1.70.0
## [25] stringr_1.4.0 digest_0.6.27
## [27] rmarkdown_2.6 XVector_0.28.0
## [29] pkgconfig_2.0.3 htmltools_0.5.1.1
## [31] extrafont_0.17 maps_3.3.0
## [33] fastmap_1.1.0 limma_3.44.3
## [35] highr_0.8 rlang_0.4.10
## [37] rstudioapi_0.13 RSQLite_2.2.3
## [39] DelayedMatrixStats_1.10.1 gridGraphics_0.5-1
## [41] generics_0.1.0 farver_2.0.3
## [43] BiocParallel_1.22.0 dplyr_1.0.3
## [45] RCurl_1.98-1.2 BiocSingular_1.4.0
## [47] GenomeInfoDbData_1.2.3 Matrix_1.3-2
## [49] Rcpp_1.0.6 munsell_0.5.0
## [51] fansi_0.4.2 lifecycle_0.2.0
## [53] stringi_1.5.3 yaml_2.2.1
## [55] MASS_7.3-53 zlibbioc_1.34.0
## [57] plyr_1.8.6 grid_4.0.3
## [59] blob_1.2.1 dqrng_0.2.1
## [61] crayon_1.3.4 lattice_0.20-41
## [63] splines_4.0.3 annotate_1.66.0
## [65] hms_1.0.0 locfit_1.5-9.4
## [67] knitr_1.31 pillar_1.4.7
## [69] geneplotter_1.66.0 reshape2_1.4.4
## [71] XML_3.99-0.5 glue_1.4.2
## [73] evaluate_0.14 BiocManager_1.30.10
## [75] vctrs_0.3.6 Rttf2pt1_1.3.8
## [77] gtable_0.3.0 purrr_0.3.4
## [79] tidyr_1.1.2 assertthat_0.2.1
## [81] cachem_1.0.1 xfun_0.20
## [83] rsvd_1.0.3 xtable_1.8-4
## [85] survival_3.2-7 tibble_3.0.1
## [87] rvcheck_0.1.8 memoise_2.0.0
## [89] ellipsis_0.3.1
Blighe and Lun (2019)
Blighe (2013)
Horn (1965)
Buja and Eyuboglu (1992)
Lun (2019)
Gabriel (1971)
Blighe, K. 2013. “Haplotype classification using copy number variation and principal components analysis.” The Open Bioinformatics Journal 7:19-24.
Blighe, K, and A Lun. 2019. “PCAtools: everything Principal Components Analysis.” https://github.com/kevinblighe/PCAtools.
Buja, A, and N Eyuboglu. 1992. “Remarks on Parallel Analysis.” Multivariate Behav. Res. 27, 509-40.
Gabriel, KR. 1971. “The Biplot Graphic Display of Matrices with Application to Principal Component Analysis 1.” Biometrika 58 (3): 453–67. http://biomet.oxfordjournals.org/content/58/3/453.short.
Horn, JL. 1965. “A rationale and test for the number of factors in factor analysis.” Psychometrika 30(2), 179-185.
Lun, A. 2019. “BiocSingular: Singular Value Decomposition for Bioconductor Packages.” R package version 1.0.0, https://github.com/LTLA/BiocSingular.