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Zhang, Feng edited this page Jun 13, 2019 · 39 revisions
  • A reference-based toolkit for single-cell analysis

  • A web resource of references. Visit dbRef

Table of content

Citation

Feng Zhang, Ying Peng, Yaguang Dou, Rohit R Rao, Q. Richard Lu, Weidong Tian; SCREF: a reference-based solution for single-cell analysis, Coming Soon

Download

Whole repository:

Download: https://github.com/jumphone/scRef/archive/master.zip

Only "scRef.R":

curl https://raw.githubusercontent.com/jumphone/scRef/master/scRef.R > scRef.R

Use scRef without downloading:

source('https://raw.githubusercontent.com/jumphone/scRef/master/scRef.R')

Requirement

R: 3.5.0

Denpendent R packages: pcaPP, MASS, ggplot2, igraph, pastecs

Input format

Single-cell and reference expression matrix must have header (cell) and row names (gene).

For reference expression matrix, we recommend using TPM, FPKM, RPKM, RPK, UMI (which can be used to compare the expression levels of different genes) matrix.

For single-cell expression matrix, we recommend using UMI (or TPM, FPKM, RPKM, RPK) matrix.

Usage

License

Copyright (c) 2019 Zhang, Feng

Permission is hereby granted, free of charge, to any person obtaining a copy
of this software and associated documentation files (the "Software"), to deal
in the Software without restriction, including without limitation the rights
to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
copies of the Software, and to permit persons to whom the Software is
furnished to do so, subject to the following conditions:

The above copyright notice and this permission notice shall be included in all
copies or substantial portions of the Software.

THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
SOFTWARE.
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