8000 [TERM REQUEST] WST-8 · Issue #298 · enanomapper/ontologies · GitHub
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[TERM REQUEST] WST-8 #298

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egonw opened this issue Jul 2, 2021 · 1 comment · Fixed by #359
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[TERM REQUEST] WST-8 #298

egonw opened this issue Jul 2, 2021 · 1 comment · Fixed by #359
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@vedina @jmillanacosta
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enhancement SbD4Nano
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June 2023 Release

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@egonw
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egonw commented Jul 2, 2021

What would you use as label or labels for the terms?

Like WST-1, unsure what difference is.
@jmillanacosta jmillanacosta self-assigned this Jun 19, 2023
@jmillanacosta jmillanacosta added this to the June 2023 Release milestone Jun 19, 2023
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unsure what difference is.*

WST-8: [O-]S(C1=CC(S([O-])(=O)=O)=C(C2=NN(C3=CC=C(N+=O)C=C3)N+=N2)C=C1)(=O)=O.[Na+]
WST-1: [O-]S(C(C=C1)=CC(S([O-])(=O)=O)=C1C2=NN(C3=CC=C(I)C=C3)N+=N2)(=O)=O.[Na+]

image

The extracellular conversion of tetrazolium salts to formazan products is based on the activity of intracellular mitochondrial oxidoreductases and the reduction of NADH/NADPH in viable cells [1]

Different reagent, same outcome: assess induction/inhibition of cell proliferation [2,3]

  1. Lutter, A.-H., Scholka, J., Richter, H., & Anderer, U. (2017). Applying XTT, WST-1, and WST-8 to human chondrocytes: A comparison of membrane-impermeable tetrazolium salts in 2D and 3D cultures. Clinical Hemorheology and Microcirculation, 67(3–4), 327–342. https://doi.org/10.3233/CH-179213
  2. https://www.caymanchem.com/product/10010199/wst-8-cell-proliferation-assay-kit
  3. https://www.caymanchem.com/product/10008883/wst-1-cell-proliferation-assay-kit

Adding it next to WST-1 now

jmillanacosta added a commit that referenced this issue Jun 19, 2023
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Updated with WST-1 and 8 assays
7D6E
80ef73b 
solves #298
@jmillanacosta jmillanacosta linked a pull request Jun 19, 2023 that will close this issue
Updated with WST-1 and 8 assays #359
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This was referenced Jun 19, 2023
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@vedina vedina

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enhancement SbD4Nano
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Updated with WST-1 and 8 assays enanomapper/ontologies
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