Tags: chenaifang/relion
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Use "Slower, more exhaustive search" in CTFFIND 4.1 unless --fast_sea… …rch is ON. "Slower search" was OFF by default in CTFFIND 4.1.5, but turned out to be less accurate (ref: http 673D ://grigoriefflab.janelia.org/ctffind4).
v.2.1-beta-1 (2.1.b1) This fixes a critical bug affecting half-set (gold-standard) refinement. Starting with version 2.0.4, each iteration of auto-refinement incorrectly uses the same half-set reconstruction as for alignment of all data. The FSC and resolution reported is however still based on comparing two half-set reconstructions which contain completely separate image data. A degree of reference-bias is however present. The effect is to report a resolution higher than what should be under gold-standard conditions, since, reconstructions are subject to a degree of over-fitting. In order to claim a so-called "gold-standard" resolution, use any version before and including 2.0.3, or version 2.1b1 (this release).