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R package (compatible with SCope) to create generic .loom files and extend them with other data e.g.: SCENIC regulons, Seurat clusters and markers, ...

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SCopeLoomR v0.3.1

An R package (compatible with SCope) to create generic .loom files and extend them with other data e.g.: SCENIC regulons, Seurat clusters and markers, ... The package can also be used to extract data from .loom files.

Requirements

  • HDF5 >= 1.10.1

For Linux and MacOS machines, version 1.10.1 can be installed with this snippet:

curl -O https://support.hdfgroup.org/ftp/HDF5/releases/hdf5-1.10/hdf5-1.10.1/src/hdf5-1.10.1.tar
cd hdf5-1.10.1
./configure
make -j4
make check
make install

To install HDF5 on a Windows machine, please use the prebuilt binaries available at https://www.hdfgroup.org/downloads/hdf5/.

For other HDF5 releases, please visit https://support.hdfgroup.org/ftp/HDF5/releases/.

Installation

Installation should take less than one minute.

install.packages("devtools")
library(devtools)
install_github("aertslab/SCopeLoomR")

Tutorial

You can find a tutorial on how to create .loom files and extract data from them here.

Version History

November 8, 2018

  • Version 0.3.1

    • Fix bug when adding sparse matrices.
  • Version 0.3.0

    • Add feature to extract the gene expression matrix of a given cluster.
    • Update tutorial.

October 31, 2018

  • Version 0.2.2
    • Add sanity tests when adding embeddings and SCENIC regulons with threshold assignments.
    • Small bug fixes.

August 8, 2018

  • Version 0.2.1
    • Fix encoding bug leading to annotation not shown in SCope.
    • Add sanity test to check that all cells from the dgem are present in the Seurat clusters.

July 6, 2018

  • Version 0.2.0
    • Add feature to store trajectory data. Currently, the following softwares can have their trajectory data easily stored in .loom files: monocle.

July 4, 2018

  • Version 0.1.0
    • Add feature to store metrics

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R package (compatible with SCope) to create generic .loom files and extend them with other data e.g.: SCENIC regulons, Seurat clusters and markers, ...

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