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🦠 Amplicon 16S

qiime2.sh

  • Preprocess
  • Quality control & filtering
  • Assign taxonomy
  • Diversity analysis
  • Phylogenetic tree

Create visualizations

R - qiimer2, phyloseq & metagenomeSeq

  • Sample composition plots
  • Alpha & Beta diversity
  • Differential abundance analysis (for zero inflated data)

Python - Microbiome gene set enrichment

  • Microbiome set enrichment analysis with MSEA
  • Pathways enrichment analysis with GSEApy
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