Azotobacter vinelandii is Gram-negative diazotroph that can fix nitrogen while grown aerobically.[2][3] These bacteria are easily cultured and grown.
| Azotobacter vinelandii | |
|---|---|
Scientific classification 
| |
| Domain: | Bacteria |
| Phylum: | Pseudomonadota |
| Class: | Gammaproteobacteria |
| Order: | Pseudomonadales |
| Family: | Pseudomonadaceae |
| Genus: | Azotobacter |
| Species: | A. vinelandii
|
| Binomial name | |
| Azotobacter vinelandii | |
A. vinelandii is a free-living N2 fixer known to produce many phytohormones and vitamins in soils. It produces fluorescent pyoverdine pigments.[4]
Nitrogenase
editThe nitrogenase holoenzyme of A. vinelandii has been characterised by X-ray crystallography in both ADP tetrafluoroaluminate-bound[5] and MgATP-bound[6] states. The enzyme possesses molybdenum iron-sulfido cluster cofactors (FeMoco) as active sites, each bearing two pseudocubic iron-sulfido structures.
Applications
editIt is a genetically tractable system that is used to study nitrogen fixation.
Genetically engineered strains can produce significantly higher amounts of ammonia. Appropriate ammonia emissions can provide crops with the ammonia they need without excess amounts that can pollute lakes and oceans.[7]
A. vinelandii also produces significant amounts of alginate.[8]
Variable ploidy
editA. vinelandii can contain up to 80 chromosome copies per cell.[9] However this is only seen in fast growing culture, whereas cultures grown in synthetic minimal media are not polyploid.[10]
References
edit- ^ William A. Noyes, ed. (1904). Review of American Chemical Research. Vol. 10. p. 75.
- ^ Young, Mark. "Why it is possible to reduce Nitrogen fertilizers by using Azotobacter sp". Retrieved 14 June 2018.
- ^ Requena N, Baca TM, Azcon R (1997). "Evolution of humic substances from unripe compost during incubation with lignolytic or cellulolytic microorganisms and effects on the lettuce growth promotion mediated by Azotobacter chroococcum". Biol Fertil Soils. 24: 59–65. doi:10.1007/BF01420221. S2CID 29624954.
- ^ Menhart N, Thariath A, Viswanatha T (1991). "Characterization of the pyoverdines of Azotobacter vinelandii ATCC 12837 with regard to heterogeneity". Biology of Metals. 4 (4): 223–32. doi:10.1007/bf01141185. PMID 1838001. S2CID 8712926.
- ^ Schindelin H, Kisker C, Schlessman JL, Howard JB, Rees DC (1997). "Structure of ADP x AIF4(-)-stabilized nitrogenase complex and its implications for signal transduction". Nature. 387 (6631): 370–376. doi:10.1038/387370a0. PMID 9163420. S2CID 1582534.
- ^ Chiu H, Peters JW, Lanzilotta WN, Ryle MJ, Seefeldt LC, Howard JB, Rees DC (2001). "MgATP-Bound and nucleotide-free structures of a nitrogenase protein complex between the Leu 127 Delta-Fe-protein and the MoFe-protein". Biochemistry. 40 (3): 641–650. doi:10.1021/bi001645e. PMID 11170380.
- ^ Coxworth, Ben (2022-02-18). "Engineered ammonia-producing bacteria could replace crop fertilizers". New Atlas. Retrieved 2022-02-21.
- ^ Clementi, Franceses (1997). "Alginate Production by Azotobacter Vinelandii". Critical Reviews in Biotechnology. 17 (4).
- ^ Nagpal P, Jafri S, Reddy MA, Das HK (1989). "Multiple chromosomes of Azotobacter vinelandii". J. Bacteriol. 171 (6): 3133–8. doi:10.1128/jb.171.6.3133-3138.1989. PMC 210026. PMID 2785985.
- ^ Maldonado R, Jiménez J, Casadesús J (1994). "Changes of ploidy during the Azotobacter vinelandii growth cycle" (PDF). J. Bacteriol. 176 (13): 3911–9. doi:10.1128/jb.176.13.3911-3919.1994. PMC 205588. PMID 8021173.
External links
edit- Azotobacter vinelandii Genome Project
- Current research on Azotobacter vinelandii at the Norwich Research Park
- Type strain of Azotobacter vinelandii at BacDive - the Bacterial Diversity Metadatabase