SARS-COV-۲ ORF۹b Dysregulate Fibrinogen and Albumin Genes in a Liver Cell Line
Publish Year: 1403
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_RBMB-13-1_006
تاریخ نمایه سازی: 18 آبان 1403
Abstract:
Background: Individuals experiencing severe cases of Coronavirus Disease ۲۰۱۹ (COVID-۱۹) exhibited elevated fibrinogen levels and decreased albumin levels, potentially linked to the presence of Severe Acute Respiratory Syndrome Coronavirus ۲ (SARS-CoV-۲) proteins. Consequently, our study endeavors to examine the impact of SARS-CoV-۲ ORF۹b on the expression of fibrinogen and albumin genes within the Hep-G۲ cell line.
Methods: In this study, the Hep-G۲ liver cell line was utilized alongside the plasmid pcDNA۳.۱ hyg+ containing ORF۹b from the SARS-CoV-۲ strain originating in Wuhan. Transfection procedures were executed, and the transfected cells were selected utilizing hygromycin B. Validation of ORF۹b expression was conducted through SYBR green-based real-time PCR, and the expression of the Fibrinogen α (FGA), Fibrinogen β (FGB), Fibrinogen γ (FGG), and Albumin (ALB) genes was quantified using the same method.
Results: The real-time PCR analysis revealed a significant upregulation of fibrinogen genes—α (P=۰.۰۳), β (P=۰.۰۲), and γ (P=۰.۰۲۹) in Hep-G۲ cells containing ORF۹b compared to control cells. Furthermore, the findings indicated a markedly lower expression level of albumin in Hep-G۲ cells harboring ORF۹b compared to the control cells (P=۰.۰۲۸).
Conclusion: The findings suggest that SARS-CoV-۲ ORF۹b could potentially influence the course of SARS-CoV-۲ infection by triggering the expression of α, β, and γ fibrinogen gene chains while suppressing the albumin gene. Further investigations are warranted to validate these observations across various SARS-CoV-۲ strains exhibiting differing levels of pathogenicity.
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Authors
Shirin Jalili
Institute of police equipment and technologies, policing sciences and social studies research institute, Tehran, Iran.
Seyed Mohammad Ali Hashemi
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Jamal Sarvari*
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran & Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
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