Evaluation the effect of honey bee venom on dermal fibroblast cells

Skin aging is considered as one of the problems of skin health, and prevention this process with anti-aging skin strategies is one of the attractive branches in dermatology.In recent years, extensive researches have been conducted on various anti-aging mechanisms, specially endogenous mechanisms and numerous treatment strategies have been provided.Based on the structure of the skin, which includes three layers: epidermis, dermis and subcutaneous tissues, there are different types of cells in these layers. In the dermis layer most of the cells are fibroblasts which form the extracellular matrix by producing elastin and collagen, and their proliferation and population decrease significantly in the aging process of the skin. In recent years, using the natural ingredients in cosmetics such as bee venom (BV) has been significantly developed. According to the studies, BV is an effective substance in healing wounds due to its renovation, anti-inflammatory and antimicrobial properties. Therefore, the aim of this study is to investigate the effect of bee venom on stimulating the proliferation of dermal fibroblast cells(DF).In this study first we analyzed BV through HPLC-UV in order to standardization and afterwards we treated DFs with different concentrations of BV in different time periods, finally evaluated proliferation of cells by MTT assay.The most effective concentration of BV was observed at ۰.۵ μg/ml and during ۲۴-hour treatment. These results suggest the possibility of using BV as an API for antiaging skin products.