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A DNA sequence specific for forest form Onchocerca volvulus

Nature volume 327, pages 415–417 (1987)Cite this article

Abstract

Onchocerciasis, or river blindness, is caused by infection with Onchocerca volvulus, a filarial parasite which infects about 40 million people in Africa and Latin America. Epidemiological1, clinical2, entomological3,4 and serological5 studies of African Onchocerciasis led to the hypothesis that Onchocerca volvulus exists in different forms in the forest and savannah. It is uncertain if these differences are due to genetic differences within O. volvulus itself, or to epigenetic factors, such as differences in the host populations. To date no basic biochemical differences between the forest and savannah populations of O. volvulus has been found, although isoenzyme studies have shown that differences in allele frequency between forest and savannah populations exist6,7. Here we describe the isolation of a DNA sequence that seems to be specific for the forest form of O. volvulus, the first indication of a basic genetic difference between the savannah and forest forms.

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References

  1. Brumpt, E. Bull. Soc. Path. exot. 12, 446–473 (1919).

    Google Scholar 

  2. Anderson, J. et al. Trans. R. Soc. trop. Med. Hyg. 68, 209–222 (1974).

    Article  CAS  Google Scholar 

  3. Duke, B. O. L. & Anderson, J. Z. Tropenmed. Parasit. 23, 354–368 (1972).

    CAS  Google Scholar 

  4. Duke, B. O. L. et al. Ann. trop. Med. Parasit. 60, 318–336 (1966).

    Article  CAS  Google Scholar 

  5. Lobos, E. & Weiss, N. Para. Immun. 7, 333–347 (1985).

    Article  CAS  Google Scholar 

  6. Cianchi, R. et al. Acta Trop. 42, 341–351 (1985).

    CAS  PubMed  Google Scholar 

  7. Flockhart, H. A., Cibulskis, R. E., Karam, M. & Albiez, E. J. Trans. R. Soc. trop. Med. Hyg. 80, 285–292 (1986).

    Article  CAS  Google Scholar 

  8. Perler, F. B. & Karam, M. Molec. Biochem. Parasit. 21, 171–178 (1986).

    Article  CAS  Google Scholar 

  9. Harnett, W., Chambers, A. & Parkhouse, R. M. E. J. cell. Biochem. 11A (suppl.) 158 (1987).

    Google Scholar 

  10. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

  11. Unnasch, T. R. & Wirth, D. F. Nucleic Acids Res. 11, 8443–8459 (1983).

    Article  CAS  Google Scholar 

  12. Sanger, F. et al. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Division of Geographic Medicine, Department of Medicine, and Department of Molecular Biology and Microbiology, Case Western Reserve University and University Hospitals, Cleveland, Ohio, 44106, USA

    K. D. Erttmann, T. R. Unnasch & B. M. Greene

  2. Bernhard-Nocht-Institut für Schiffs- und Tropenkrankheiten, D-2000, Hamburg, FRG

    E. J. Albiez

  3. Liberian Agricultural Company, Grand Bassa County, Liberia

    J. Boateng & P. N. Williams

  4. Institut National D'Hygiene, Lomé, Togo

    A. M. Denke

  5. Institute de Medecina Tropical de Manaus, CEP: 69,000, Manaus Amazonas, Brazil

    J. J. Ferraroni

  6. Onchocerciasis Control Program, Ouagadougou, Burkina Faso

    M. Karam

  7. Tropenmedizinisches Institut, Universitat Tübingen, D-7400, Tübingen, FRG

    H. Schulz–Key

Authors
  1. K. D. Erttmann
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  2. T. R. Unnasch
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  3. B. M. Greene
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  4. E. J. Albiez
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  5. J. Boateng
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  6. A. M. Denke
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  7. J. J. Ferraroni
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  8. M. Karam
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  9. H. Schulz–Key
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  10. P. N. Williams
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Erttmann, K., Unnasch, T., Greene, B. et al. A DNA sequence specific for forest form Onchocerca volvulus. Nature 327, 415–417 (1987). https://doi.org/10.1038/327415a0

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