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Evaluation of Fructooligosaccharide Supplementation of Oral Electrolyte Solutions for Treatment of Diarrhea (Recovery of the Intestinal Bacteria)

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Abstract

Although oral electrolyte solutions (OES)replenish salts and water lost during diarrhea, presentformulations do not address disturbances of the normalintestinal microbiota. Therefore, we evaluated the efficacy of an OES with and withoutfructooligosaccharide (FOS) for treatment of pigs withacute secretory diarrhea induced by cholera toxin.Before, during, and after diarrhea, bacteriologicevaluation was made of contents collected from the midsmall intestine, cecum, and distal colon and mucosascraped from the mid small intestine. Diarrhea causedsignificant declines in total bacterial counts ofcontents from all three regions, with less of an impacton bacteria associated with the mucosa. Although totalbacterial counts recovered within 24 hr, regardless oftreatment, densities of Enterobacteriaceae were higher in pigs treated with OES whereas thosereceiving FOS had more lactobacilli. Our results showthat secretory diarrhea disturbs the normal densitiesand relative species abundance of the microbiota, with the influences more pronounced forcontents relative to the mucosa, and that adding FOS toOES accelerates the recovery of bacteria perceived asbeneficial while potentially slowing the recovery of pathogenic forms.

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REFERENCES

  1. Grace y M: Bacterial causes of acute and chronic diarrhea in infants and children. InTextbook of Gastroenterology and Nutrition in Infancy. E. Lebenthal, (ed). New York, Rave n Press, 1989, pp 1121–1133

    Google Scholar 

  2. Cohen MB: Etiology and mechanism of acute infectious diarrhea in infants in the United States. J Pediatr 118:S34–S39, 1991

    Google Scholar 

  3. Goodman LJ: Diagnosis, management, and prevention of diarrhe al disease s. Curr Opin Infect Dis 6:88–93, 1993

    Google Scholar 

  4. Hirschhorn N, Greenough WB, III: Progress in oral rehydration therapy. Sci Am 264:50–56, 1991

    Google Scholar 

  5. Greenough WB, III: Rice based oral rehydration solution. Br Med J 304:982, 1992 ( letter)

    Google Scholar 

  6. Hentges DJ: Human Intestinal Microflora in Health and Disease. New York, Academic Press, 1983

    Google Scholar 

  7. Mitsuoka T: Effects of long-term intake of neosugar on intestinal flora and serum lipids. Proceedings of the 3rd Neosugar Conference, Tokyo, 1986

  8. Tannock GW: The microe cology of lactobacilli inhabiting the gastrointestinal tract. Adv Microbial Ecol 11:147–171, 1990

    Google Scholar 

  9. Link-Amste r H, Rochat F, Saudan KY, Mignot O, Aeschlimann JM: Modulation of a specific humoral immune re sponse and changes in intestinal flora mediated through fermented milk intake. FEMS Immunol Med Microbiol 10:55–63, 1994

    Google Scholar 

  10. Homma N: Bifidobacteria as a resistance factor in human beings. Bif Microflora 7:35–43, 1988

    Google Scholar 

  11. Ueda K: Immunity provided by colonized enteric bacteria. Bif Microflora 5:67–72, 1976

    Google Scholar 

  12. Chadwick RW, George SE, Claxton LD: Role of the gastrointestinal mucosa and microflora in the bioactivation of dietary and environmental mutagens or carcinoge ns. Drug Metab Rev 24:425–492, 1992

    Google Scholar 

  13. Gorbach SL, Goldin BR: Nutrition and the gastrointestinal microflora. Nutr Rev 50:378–381, 1992

    Google Scholar 

  14. Gorbach SL, Banwell JG, Jacobs B, Chatterjee BD, Mitra R, Brigham KL, Neogy KN: Intestinal microflora in Asiatic cholera. I. “Rice-water” stool. J Infect Dis 121:32–37, 1970

    Google Scholar 

  15. Gorbach SL, Banwell JG, Jacobs B, Chatterjee BD, Mitra R, Brigham KL, Neogy KN: Intestinal microflora in Asiatic cholera. II. The small bowel. J Infect Dis 121:38–45, 1970

    Google Scholar 

  16. Mata LJ, Mejicanos ML, Jimenez F: Studies on the indigenous gastrointestinal flora of Guatemalan children. Am J Clin Nutr 25:1380–1390, 1972

    Google Scholar 

  17. Tannock GW: Effects of dietary and environmental stre ss on the gastrointestinal microbiota. InHuman Intestinal Microflora in Health and Disease. New York, Academic Press, 1983

    Google Scholar 

  18. Gibson GR, Roberfroid MB: Die tary modulation of the human colonic microflora: Introducing the concept of prebiotics. J Nutr 125:1401–1412, 1995

    Google Scholar 

  19. Tannock GW: The normal microflora: New concepts in health promotion. Microbiol Sci 5:4–8, 1988

    Google Scholar 

  20. Fuller R: Probiotics, The Scientific Basis. New York, Chapman and & Hall, 1992

    Google Scholar 

  21. Isolauri E, Juntunen M, Rautanen T, Sillanaukee P, Kiovula T: A human Lactobacillusstrain (Lactobacillus caseisp. strain GG) promotes recovery from acute diarrhea in children. Pediatrics 88:90–97, 1991

    Google Scholar 

  22. Isolauri E, Kaila M, Mykkänen H, Ling WH, Salminen S: Oral bacteriotherapy for viral gastroente ritis. Dig Dis Sci 39:2595–2600, 1994

    Google Scholar 

  23. Buddington RK, Williams CH, Chen S, Witherly SW: A dietary supplement of neosugar alters the fecal flora and decreases activities of some reductive enzymes in human subjects. Am J Clin Nutr 63:709-716, 1996

    Google Scholar 

  24. Hidaka H, Eida T, Takizawa T, Tokunga T, Tashiro Y: Effects of fructooligosaccaride s on intestinal flora and human health. Bif Microflora 5:37–50, 1986

    Google Scholar 

  25. Mitsuoka T: Intestinal flora and aging. Nutr Rev 50:438–446, 1992

    Google Scholar 

  26. Williams CH, Witherly SW, Buddington RK: Influence of dietary neosugar on se lected bacte rial groups of the human fecal flora. Microb Ecol Health Dis 7:91–97, 1994

    Google Scholar 

  27. Farnworth ER, Dilawri N, Yamazaki H, Modler HW, Jones JD: Studies on the effect of adding Jerusalem artichoke flour to pig milk replace r. Can J Anim Sci 71:531–536, 1991

    Google Scholar 

  28. Ahmed ZU, Sarker MR, Sack DA: Protection of adult rabbits and monkeys from lethal shigellosis by oral immunization with a thymine-requiring and temperature-sensitive mutant of Shigella flexneriY. Vaccine 8:153–158, 1990

    Google Scholar 

  29. Campbell JM, Fahe y GC Jr, Wolf BW: Selected indigestible oligosaccharides affect large bowel mass and fecal short-chain fatty acids, pH and microflora in rats. J Nutr 127:130–136, 1997

    Google Scholar 

  30. Gibson GR, Wang X: Regulatory effects of bifidobacteria on the growth of other colonic bacteria. J Appl Bacteriol 77:412–420, 1994

    Google Scholar 

  31. Moughan PJ, Cranwell PD, Darragh AJ, Rowan AM: The domestic pig as a mode l animal for studying digestion in humans. VIth International Symposium on Digestive Physiology in Pigs II. 1994, pp 389–396

  32. Hunt JB, Thillainayagam AV, Carnaby S, Fairblough PD, Clark ML, Farthing MJG: A new model of human secretory diarrhoea using cholera toxin. Aliment Pharmacol The r 6:619–627, 1992

    Google Scholar 

  33. Summanen P, Baron EJ, Citron DM, Strong C, Wexler HM, Finegold SM: Wadsworth Anaerobic Bacteriology Manual, 3rd ed. Los Ange les, Star Publ. Co., 1993

    Google Scholar 

  34. Sutter VL, Citron DM, Edelstein MAC, Finegold SM: Wadsworth Anaerobic Bacteriology Manual. Belmont, California, Star Publ. Co., 1986

    Google Scholar 

  35. Munoa FJ, Pare s R: Selective medium for isolation and enumeration of Bifidobacteriumspp. Appl Environ Microbiol 54:1715–1718, 1988

    Google Scholar 

  36. Scardovi V: Genus Bifidobacterium. InBergey's Manual of Determinative Bacteriology. Baltimore, Williams & Wilkins, 1986, pp 1418-1434

    Google Scholar 

  37. Howard MD, Gordon DT, Pace LW, Garleb KA, Kerley MS: Effects of dietary supplementation with fructooligosaccharides on colonic microbiota populations and epithelial cell proliferation in neonatal pigs. J Pediatr Gastroenterol Nutr 21:297-303, 1995

    Google Scholar 

  38. Mitsuoka T, Hidaka H, Eida T: Effect of fructo-oligosaccharides on intestinal microflora. Die Nahrung 31:427–436, 1987

    Google Scholar 

  39. Perman JA: Gastrointestinal flora: Developmental aspects and effects of nutrients. InHuman Gastrointestinal Development. E. Lebenthal (ed). New York, Raven Press, 1989 pp 777-786

    Google Scholar 

  40. Swords WE, Wu CC, Champlin FR, Buddington RK: Postnatal changes in selected bacterial groups of the pig colonic microflora. Biol Neonate 63:191–200, 1993

    Google Scholar 

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Oli, M.W., Petschow, B.W. & Buddington, R.K. Evaluation of Fructooligosaccharide Supplementation of Oral Electrolyte Solutions for Treatment of Diarrhea (Recovery of the Intestinal Bacteria). Dig Dis Sci 43, 138–147 (1998). https://doi.org/10.1023/A:1018892524790

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