[go: up one dir, main page]
More Web Proxy on the site http://driver.im/
Skip to main content
Springer Nature Link
Log in

Cardioprotective effect of resveratrol analogue isorhapontigenin versus omega-3 fatty acids in isoproterenol-induced myocardial infarction in rats

  • Original Paper
  • Published:
Journal of Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

Myocardial infarction (MI) is a common cause of mortality worldwide. Isorhapontigenin is a derivative of stilbene with chemical structure similar to resveratrol. The omega-3 fatty acids (FA) have beneficial effects on neurodegenerative, inflammatory, and cardiovascular diseases. The aim of this study was to investigate the effects of pretreatment with isorhapontigenin and omega-3 FA on rat model of isoproterenol-induced MI. Fifty-six rats were divided into seven groups: normal, normal + isorhapontigenin, normal + omega-3 FA, MI, MI + isorhapontigenin, MI + omega-3 FA, and MI + isorhapontigenin + omega-3 FA. Serum levels of cardiac marker enzymes [lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB)], cardiac troponin I (cTnI), inflammatory markers [tumor necrosis factor-alpha (TNF-α) and interleukin-6], and lipid profile [triglycerides, total cholesterol (T.Ch), high and low density lipoproteins (HDL, LDL), and phospholipids] as well as cardiac levels of malondialdehyde and anti-oxidants [reduced glutathione (GSH), superoxide dismutase (SOD), and catalase)] were measured in all rats. ECG and histopathological examination were performed. Isoproterenol caused a significant elevation of ST segment, decreased R wave amplitude, HDL, and anti-oxidants, and increased LDH, CK-MB, cTnI, TNF-α, interleukin-6, malondialdehyde, triglycerides, T.Ch, LDL, and phospholipids. Omega-3 FA or isorhapontigenin significantly decreased the ST segment elevation, LDH, CK-MB, cTnI, TNF-α, interleukin-6, malondialdehyde, and phospholipids and increased R wave amplitude and anti-oxidants. The effects of combined omega-3 FA and isorhapontigenin were more significant than either of them alone. Therefore, we conclude that omega-3 FA and isorhapontigenin have a cardioprotective effect on rats with isoproterenol-induced MI through their anti-oxidant and anti-inflammatory actions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
£29.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (United Kingdom)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Adkins Y, Kelley DS (2010) Mechanisms underlying the cardioprotective effects of omega-3 polyunsaturated fatty acids. J Nutr Biochem 21:781–792

    Article  CAS  PubMed  Google Scholar 

  2. Baumann KH, Hessel F, Larass I et al (1999) Dietary -3, -6 and -9 unsaturated fatty acids and growth factor and cytokine gene expression in unstimulated and stimulated monocytes. Arterioscler Thromb Vasc Biol 19:59–66

    Article  CAS  PubMed  Google Scholar 

  3. Bosch J, Gerstein HC, Dagenais GR et al (2012) N-3 fatty acids and cardiovascular outcomes in patients with dysglycemia. N Engl J Med 367(4):309–318

    Article  CAS  PubMed  Google Scholar 

  4. Burr ML, Ashfield-Watt PA, Dunstan FD, Fehily AM, Breay P, Ashton T, Zotos PC, Haboubi NA, Elwood PC (2003) Lack of benefit of dietary advice to men with angina: results of a controlled trial. Eur J Clin Nutr 57:193–200

    Article  CAS  PubMed  Google Scholar 

  5. Chen YR, Yi FF, Li XY, Wang CY, Chen L, Yang XC, Su PX, Cai JR (2008) Resveratrol attenuates ventricular arrhythmias and improves the long-term survival in rats with myocardial infarction. Cardiovasc Drugs Ther 22:479–485

    Article  CAS  PubMed  Google Scholar 

  6. Connor WE (2000) Importance of n-3 fatty acids in health and disease. Am J Clin Nutr 71:171S–175S

    CAS  PubMed  Google Scholar 

  7. Cosar M, Songur A, Sahin O, Uz E, Yilmaz R, Yagmurca M et al (2008) The neuroprotective effect of fish n-3 fatty acids in the hippocampus of diabetic rats. Nutr Neurosci 11:161–166

    Article  CAS  PubMed  Google Scholar 

  8. El-Gendy AA, Abbas AM (2014) Effect of omega-3 fatty acids on haemostatic functions in urocortin-treated obese rats. J Physiol Biochem 70:809–820

    Article  CAS  PubMed  Google Scholar 

  9. Eleawa SM, Alkhateeb M, Ghosh S, Al-Hashem F, Shatoor AS, Alhejaily A, Khalil MA (2015) Coenzyme Q10 protects against acute consequences of experimental myocardial infarction in rats. Int J Physiol Pathophysiol Pharmacol 7(1):1–13

    PubMed  PubMed Central  Google Scholar 

  10. Fernández-Marín MI, Guerrero RF, García-Parrilla MC, Puertas B, Richard T, Rodriguez-Werner MA, Winterhalter P, Monti JP, Cantos-Villar E (2012) Isorhapontigenin: a novel bioactive stilbene from wine grapes. Food Chem 135:1353–1359

    Article  PubMed  Google Scholar 

  11. Fox KAA, Steg PG, Eagle KA et al (2007) Decline in rates of death and heart failure in acute coronary syndromes, 1999–2006. JAMA 297(17):1892–1900

    Article  CAS  PubMed  Google Scholar 

  12. Hirafuji M, Machida T, Hamaue N, Minami M (2003) Cardiovascular protective effects of n-3 polyunsaturated fatty acids with special emphasis on docosahexaenoic acid. J Pharmacol Sci 92:308–316

    Article  CAS  PubMed  Google Scholar 

  13. Hooper L, Thompson RL, Harrison RA, Summerbell CD, Ness AR, Moore HJ et al (2006) Risks and benefits of omega 3 fats for mortality, cardiovascular disease, and cancer: systematic review. Br Med J 332:752–760

    Article  CAS  Google Scholar 

  14. Kang JX, Xiao YF, Leaf A (1995) Free, long-chain, polyunsaturated fatty acids reduce membrane electrical excitability in neonatal rat cardiac myocytes. Proc Natl Acad Sci U S A 92:3997–4001

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Kareem MA, Krushna GS, Hussain SA, Devi KL (2009) Effect of aqueous extract of nutmeg on hyperglycaemia, hyperlipidaemia and cardiac histology associated with isoproterenol-induced myocardial infarction in rats. Trop J Pharm Res 8(4):337

    Article  Google Scholar 

  16. Karthikeyan K, Bai BRS, Devaraj SN (2007) Efficacy of grape seed proanthocyanidins on serum and heart tissue lipids in rats subjected to isoproterenol-induced myocardial injury. Vascul Pharmacol 47:295–301

    Article  CAS  PubMed  Google Scholar 

  17. Kawakami S, Kinoshita Y, Maruki-Uchida H, Yanae K, Sai M, Ito T (2014) Piceatannol and its metabolite, isorhapontigenin, induce SIRT1 expression in THP-1 human monocytic cell line. Nutrients 6:4794–4804

    Article  PubMed  PubMed Central  Google Scholar 

  18. Khalil MI, Tanvir EM, Afroz R, Sulaiman SA, Gan SH (2015) Cardioprotective effects of tualang honey: amelioration of cholesterol and cardiac enzymes levels. BioMed Research International. Volume 2015, Article ID 286051

  19. Kromhout D, Giltay EJ, Geleijnse JM, Alpha Omega Trial Group (2010) n-3 fatty acids and cardiovascular events after myocardial infarction. N Engl J Med 363:2015–2026

    Article  CAS  PubMed  Google Scholar 

  20. Li H, Wang A, Huang Y et al (2005) Isorhapontigenin, a new resveratrol analog, attenuates cardiac hypertrophy via blocking signaling transduction pathways. Free Radic Biol Med 38(2):243–257

    Article  PubMed  Google Scholar 

  21. Lin JF, Lin SM, Chih CL, Nien MW, Su HH, Hu BR, Huang SS, Tsai SK (2008) Resveratrol reduces infarct size and improves ventricular function after myocardial ischemia in rats. Life Sci 83:313–317

    Article  CAS  PubMed  Google Scholar 

  22. Lobo Filho HG, Ferreira NL, de Sousa RB, de Carvalho ER, Dantas Lobo PL, Lobo Filho JG (2011) Experimental model of myocardial infarction induced by isoproterenol in rats. Rev Bras Cir Cardiovasc 26(3):469–476

    Article  PubMed  Google Scholar 

  23. London B, Albert C, Anderson ME, Giles WR, Van Wagoner DR, Balk E, Billman GE, Chung M, Lands W, Leaf A, McAnulty J, Martens JR, Costello RB, Lathrop DA (2007) Omega-3 fatty acids and cardiac arrhythmias: prior studies and recommendations for future research. Circulation 116:e320–e335

    Article  CAS  PubMed  Google Scholar 

  24. Murugesan M, Manju V (2012) Effect of luteolin on lipid peroxidation and antioxidants in acute and chronic periods of isoproterenol induced myocardial infarction in rats. J Acute Med 2:70–76

    Article  Google Scholar 

  25. Nounou HA, Deif MM, Shalaby MA (2012) Effect of flaxseed supplementation and exercise training on lipid profile, oxidative stress and inflammation in rats with myocardial ischemia. Lipids Health Dis 11:129

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Osadchii OE, Norton GR, McKechnie R, Deftereos D, Woodiwiss AJ (2007) Cardiac dilatation and pump dysfunction without intrinsic myocardial systolic failure following chronic β-adrenoreceptor activation. Am J Physiol Heart Circ Physiol 292(4):H1898–H1905

    Article  CAS  PubMed  Google Scholar 

  27. Ozen OA, Cosar M, Sahin O, Fidan H, Eser O, Mollaoglu H et al (2008) The protective effect of fish n-3 fatty acids on cerebral ischemia in rat prefrontal cortex. Neurol Sci 29:147–152

    Article  PubMed  Google Scholar 

  28. Patel V, Upaganlawar A, Zalawadia R, Balaraman R (2010) Cardioprotective effect of melatonin against isoproterenol induced myocardial infarction in rats: a biochemical, electrocardiographic and histoarchitectural evaluation. Eur J Pharmacol 644(1–3):160–168

    Article  CAS  PubMed  Google Scholar 

  29. Pervaiz S, Holme AL (2009) Resveratrol: its biologic targets and functional activity. Antioxid Redox Signal 11(11):2851–2897

    Article  CAS  PubMed  Google Scholar 

  30. Priscilla DH, Prince PSM (2009) Cardioprotective effect of gallic acid on cardiac troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in Wistar rats. Chem Biol Interact 179:118–124

    Article  CAS  PubMed  Google Scholar 

  31. Radhiga T, Rajamanickam C, Senthil S, Pugalendi KV (2012) Effect of ursolic acid on cardiac marker enzymes, lipid profile and macroscopic enzyme mapping assay in isoproterenol-induced myocardial ischemic rats. Food Chem Toxicol 50(11):3971–3977

    Article  CAS  PubMed  Google Scholar 

  32. Rallidis LS, Paschos G, Papaioannou ML et al (2004) The effect of diet enriched with α-linolenic acid on soluble cellular adhesion molecules in dyslipidaemic patients. Atherosclerosis 174:127–132

    Article  CAS  PubMed  Google Scholar 

  33. Ramesh CV, Malarvannan P, Jayakumar R, Jayasundar S, Puvanakrishnan R (1998) Effect of a novel tetrapeptide derivative in a model of isoproterenol induced myocardial necrosis. Mol Cell Biochem 187:173–182

    Article  CAS  PubMed  Google Scholar 

  34. Rizos EC, Ntzani EE, Bika E, Kostapanos MS, Elisaf MS (2012) Association between omega-3 fatty acid supplementation and risk of major cardiovascular disease events. JAMA 308:1024–1033

    Article  CAS  PubMed  Google Scholar 

  35. Rona G (1985) Catecholamine cardiotoxicity. J Mol Cell Cardiol 17:291–306

    Article  CAS  PubMed  Google Scholar 

  36. Rustan AC, Nossen JO, Christiansen EN, Drevon CA (1988) Eicosapentaenoic acid reduces hepatic synthesis and secretion of triacylglycerol by decreasing the activity of acyl-coenzyme A:1,2-diacylglycerol acyl-transferase. J Lipid Res 29:1417–1426

    CAS  PubMed  Google Scholar 

  37. Saravanan G, Ponmurugan P, Sathiyavathi M, Vadivukkarasi S, Sengottuvelu S (2013) Cardioprotective activity of Amaranthus viridis Linn: effect on serum marker enzymes, cardiac troponin and antioxidant system in experimental myocardial infarcted rats. Int J Cardiol 165(3):494–498

    Article  CAS  PubMed  Google Scholar 

  38. Sharmila ST, Rajadurai M (2012) Preventive effect of Bio-Aq on cardiac markers, lipids, and membrane bound enzymes in isoproterenol-induced myocardial infarction in rats. Asian J Pharm Clin Res 5(2):107–113

    Google Scholar 

  39. Upaganlawar A, Gandhi C, Balaraman R (2009) Effect of green tea and vitamin E combination in isoproterenol induced myocardial infarction in rats. Plant Foods Hum Nutr 64:75–80

    Article  CAS  PubMed  Google Scholar 

  40. Wang H, Yang Y, Zhang H, Yan H, Wu X, Zhang C (2014) Administration of the resveratrol analogues isorhapontigenin and heyneanol-a protects mice hematopoietic cells against irradiation injuries. BioMed Research International. Volume 2014: Article ID 282657

  41. Wang QL, Lin M, Liu GT (2001) Antioxidative activity of natural isorhapontigenin. Jpn J Pharmacol 87:61–66

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We thank Doctor Abdelhadi M. Shebl, Lecturer of Pathology, Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt, for his assistance in histopathological studies.

Author information

Authors and Affiliations

  1. Department of Medical Physiology, Faculty of Medicine, Mansoura University, P.O. Box 35516, Mansoura, Egypt

    Amr M. Abbas

Authors
  1. Amr M. Abbas
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Amr M. Abbas.

Ethics declarations

Conflict of interest

The author declares that he has no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Abbas, A.M. Cardioprotective effect of resveratrol analogue isorhapontigenin versus omega-3 fatty acids in isoproterenol-induced myocardial infarction in rats. J Physiol Biochem 72, 469–484 (2016). https://doi.org/10.1007/s13105-016-0494-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13105-016-0494-4

Keywords

Search

Navigation